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Variations in the Composition of Respiratory Microbiota

NCT04747184 · Status: COMPLETED · Type: OBSERVATIONAL · Enrollment: 54

Last updated 2021-02-10

No results posted yet for this study

Summary

Induced sputum samples were obtained from 27 asthmatic patients and 27 non-asthmatic subjects. Sequencing of the V4 region of 16S rRNA gene using Illumina MiSeq was performed, followed by analysis of alpha and beta diversity.

Conditions

Interventions

OTHER

DNA extraction and 16S rRNA sequencing

DNA extraction using QIAamp® DNA mini kit (QIAGEN) was done under aseptic techniques in the sterile room for all samples according to the manufacturer instructions. The DNA extracts for the 54 samples collected (27 from asthmatic patients and 27 from healthy subjects) were stored at -80° C in a sterile Eppendorf tubes until they were sent to the Molecular Research (MR DNA) Lab (Molecular Research LP, Shallowater, TX, USA) for sequencing.

Sponsors & Collaborators

  • Applied Science Private University

    lead OTHER

Principal Investigators

  • Mohammad Al-Najjar, PhD · Faculty of Pharmacy, Applied Science Private University

Eligibility

Min Age
14 Years
Sex
ALL
Healthy Volunteers
Yes

Timeline & Regulatory

Start
2019-12-01
Primary Completion
2020-05-01
Completion
2020-09-30

Countries

  • Jordan

Study Locations

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Entities

Diseases

Read the full study record

This page highlights key information. For complete eligibility criteria, study locations, investigator contacts, and the full protocol, visit the original record on ClinicalTrials.gov.

View NCT04747184 on ClinicalTrials.gov