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Cryptic Bacteria of the Thyroid Tissue as a Possible Cause of the Pathology of This Organ

NCT04552496 · Status: UNKNOWN · Type: OBSERVATIONAL · Enrollment: 120

Last updated 2020-09-17

No results posted yet for this study

Summary

The presence of cryptic microbes has been widely documented in animal healthy deep tissues.

The thyroid gland is an organ specifically exposed to the microbial environment due to its close location to the mouth microbiome. A number of bacterial phenotypes has been detected in the inflamed thyroid gland. A question raises as to whether bacteria have not already been present in the thyroid gland before the clinical symptoms of goiter became evident.

A problem in thyroid surgery, relatively uncommon but difficult for control, is prolonged thyroidectomy wound healing with skin flap, gland bed inflammation and fibrosis. The causative bacteria may belong to the strains persistently present in the thyroid gland parenchyma. Our objective is to answer questions: a) do the goiter tissue structures contain bacteria, b) if so, which bacterial phenotypes can be identified, c) what are the genetic similarities of the thyroid and periodontal bacterial strains.

Studies are carried out in patients with non-toxic multinodular goiter, toxic multinodular goiter, Graves' disease, single adenoma, Hashimoto's disease, thyroid cancer and recurrent thyroid disease. Tissue harvested during surgery is dissected immediately after thyroidectomy into fragments of parenchyma, arteries, veins and lymph nodes and cultured on Columbia blood agar base for up to 3 weeks. In this method bacteria present in the tissue grow in their natural environment, slowly proliferate and then form the on-plate colonies. It enables detection of even single bacteria usually difficult to be identified in planktonic media. Identification of the isolated bacteria is performed. Their DNA patterns are also compared.

Conditions

  • Bacteria Infection Mechanism

Sponsors & Collaborators

  • Centre of Postgraduate Medical Education

    lead OTHER

Principal Investigators

  • Sergiusz Durowicz, MD, PhD · Centre of Postgraduate Medical Education, Warsaw, Poland

  • Marzanna Zaleska, PhD · Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland

  • Waldemar L. Olszewski, MD, PhD · Central Clinical Hospital Ministry Interior Administration, Warsaw, Poland

  • Wiesław Tarnowski, Md, PhD · Centre of Postgraduate Medical Education, Warsaw, Poland

  • Ewa Swoboda-Kopeć, MD, PhD · Medical University of Warsaw, Warsaw, Poland

Eligibility

Min Age
18 Years
Max Age
80 Years
Sex
ALL
Healthy Volunteers
No

Timeline & Regulatory

Start
2018-07-05
Primary Completion
2020-09-01
Completion
2022-09-01

Countries

  • Poland

Study Locations

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Read the full study record

This page highlights key information. For complete eligibility criteria, study locations, investigator contacts, and the full protocol, visit the original record on ClinicalTrials.gov.

View NCT04552496 on ClinicalTrials.gov