Effect of Adipose Derived Stem Cells on Survival of Fat as Filler

Summary

this research is to study the effect of Adipose Derived Stem Cells on Survival of Fat as Filler

Conditions

• Skin Rejuvenation
• Lipofilling

Interventions

PROCEDURE

lipofilling

Under local anesthesia using strict aseptic technique, a small incision will be done in the lateral aspect of the thigh or lower abdomen, through which the infiltration cannula will be introduced to inject the local anesthetic solution using the wet technique. This will be followed 15 minutes later by lipo-aspiration of 75 ml fat ) using a blunt tipped cannula under the negative suction pressure of a 60 ml syringe. 50 ml is used for preparation of microfat. then with blunt cannula,subcutaneous injection of the required volume of microfat is placed into temporal region.

PROCEDURE

lipofilling enriched with adipose tissue derived stem cells

Under local anesthesia using strict aseptic technique, a small incision will be done in the lateral aspect of the thigh or lower abdomen, through which the infiltration cannula will be introduced to inject the local anesthetic solution using the wet technique. This will be followed 15 minutes later by lipo-aspiration of 75 ml fat using a blunt tipped cannula under the negative suction pressure of a 60 ml syringe. 50 ml is used for preparation of microfat. 25 ml is used for preparation of autologous adipose tissue derived stem cells (At-ADSCs) using enzymatic digestion and differential centrifugation in the Center of Excellence for Research in Regenerative Medicine and its Application (CERRMA), Alexandria Faculty of Medicine. Subcutaneous injection of the required volume of microfat combined with stromal vascular fraction containing adipose tissue derived stem cells, is placed into temporal region.

Sponsors & Collaborators

• Alexandria University

  lead OTHER

Principal Investigators

• Carmen Ibrahim Farid Mohammed Amin, MD · Assistant Professor of Dermatology, Faculty of Medicine, Alexandria University.

• Ossama Hussein Roshdy, MD · Professor of Dermatology, Faculty of Medicine, University of Alexandria.

• Wafaa Ibrahim Abdullah, MD · Professor of Dermatology, Faculty of Medicine, University of Alexandria.

• Radwa Ali Mehanna, PhD · Assistant Professor of Physiology, Faculty of Medicine, Alexandria University

• Nader Hussein Lotfy Bayoumi., MD, FRCS · Professor of ophthalmology , Faculty of Medicine, University of Alexandria.

Study Design

Allocation

RANDOMIZED

Purpose

TREATMENT

Masking

NONE

Model

PARALLEL

Eligibility

Min Age

35 Years

Sex

FEMALE

Healthy Volunteers

Yes

Timeline & Regulatory

Start

2019-01-01

Primary Completion

2019-07-16

Completion

2019-07-16

Countries

• Egypt

Study Locations