Application of Next Generation Sequencing Technique in Pediatric Bacterial Meningitis

Summary

Pathogen identification is of paramount importance for bacterial meningitis. At present, the pathogen of bacterial meningitis is still mainly based on Gram stain and bacterial culture. However, cerebrospinal fluid (CSF) culture can be negative in children who receive antibiotic treatment prior to CSF examination.Because of the limitations of clinical laboratory testing, more than half of the central nervous system infection cases cannot be clearly diagnosed. The emergence of powerful next-generation sequencing (NGS) technology have enabled unbiased sequencing of biological samples due to its rapid turnaround time. Previous reports highlight the feasibility of applying NGS of CSF as a diagnostic method for central nervous system (CNS) infection. However, the majority of reports are comprised of single case reports and few studies have been reported in the application of NGS for pathogen detection from CSF samples of bacterial meningitis patients, especially in pediatric populations. In this study, we would like to use the NGS technology to detect directly from the CSF samples of children with bacterial meningitis and evaluate the feasibility and significance of the NGS technique on the pathogenic identification of bacterial meningitis.

Conditions

• Next Generation Sequencing
• Pediatric Bacterial Meningitis

Interventions

DIAGNOSTIC_TEST

Next Generation Sequencing

To provide rapid etiological diagnosis of patients by means of next-generation sequencing.

Sponsors & Collaborators

• Xingtai People's Hospital

  collaborator OTHER

• Baoding Children's Hospital

  collaborator OTHER

• Jinan children's hospital

  collaborator UNKNOWN

• Liaocheng People's Hospital

  collaborator OTHER

• Nanjing Children's Hospital

  collaborator OTHER

• Shenzhen Baoan Maternal and Child Care Service Centre

  collaborator UNKNOWN

• Jiangxi Children's Hospital

  collaborator UNKNOWN

• Kunming Children's Hospital

  collaborator OTHER

• First Affiliated Hospital of Xinjiang Medical University

  collaborator OTHER

• Children's Hospital Affiliated to Suzhou University

  collaborator UNKNOWN

• Beijing Children's Hospital

  lead OTHER

Principal Investigators

• Gang Liu · Beijing Children's Hospital

Eligibility

Min Age

28 Days

Max Age

18 Years

Sex

ALL

Healthy Volunteers

No

Timeline & Regulatory

Start

2018-02-01

Primary Completion

2020-01-31

Completion

2020-07-31

Countries

• China

Study Locations