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Evaluation of Different Methods for Diagnosis of ME

NCT03759470 · Status: UNKNOWN · Type: OBSERVATIONAL · Enrollment: 2

Last updated 2018-11-30

No results posted yet for this study

Summary

Bacterial meningitis occurs in about 3 people per 100,000 annually in Western countries. .

Conditions

  • Diagnoses Disease

Interventions

DIAGNOSTIC_TEST

CSF examination

The CSF samples will be stored at -80°C until testing . CSF specimens will be prepared for GS and culture examinations by centrifugation at 3,000 rpm for 10 minutes at room temperature. Culture will be performed by inoculating 1-2 drops of CSF sediment directly onto each of the following agar plates: horse-blood agar, Thayer-Martin agar, choc- olate agar and Sabouraud agar. Moreover, a broth tube (brain-heart infusion, BHI) will be inoculated with one drop of the sediment. Agar plates and broth will be incubated for 1 to 5 days at 35-37°C (with \~5% CO2, or in a candle-jar, for Thayer-Martin and chocolate agar) . In addition to GS and culture method , India ink test and culture and latex agglutination test (LAT). LAT assay will be performed on CSF samples using Latex-antigen detection system kit.

Sponsors & Collaborators

  • Assiut University

    collaborator OTHER

  • Doha Fahmy

    lead OTHER

Eligibility

Min Age
1 Day
Max Age
16 Years
Sex
ALL
Healthy Volunteers
No

Timeline & Regulatory

Start
2019-01-01
Primary Completion
2019-12-30
Completion
2019-12-31

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Read the full study record

This page highlights key information. For complete eligibility criteria, study locations, investigator contacts, and the full protocol, visit the original record on ClinicalTrials.gov.

View NCT03759470 on ClinicalTrials.gov