DNA and RNA Viruses of the Blood Virome of Allogeneic Hematopoietic Stem Cell Transplant Recipients

Summary

The Geneva Blood Virome Project is a longitudinal observational study. The main objective is to describe the kinetics of the plasmatic viral load of a selection of at least 21 DNA and RNA viruses of the blood virome in allogeneic hematopoietic stem cell transplant recipients, over a one-year period after transplantation. Secondary objectives are: 1) to assess the prevalence of DNA and RNA viruses plasmatic detections and co-detections, 2) to assess the cumulative incidence of DNA and RNA viruses plasmatic detection. The population of the study consists in adult patients receiving allogeneic stem cell transplantation at the University Hospitals of Geneva, enrolled in an already existing monocentric cohort, and for which clinical specimens are collected and stored at the time and after transplantation. The investigators plan to include 120 patients whose plasma samples are collected from March 2017 and to systematically use plasma samples collected on the day of transplantation and several time points after transplantation to screen DNA and RNA viruses by qualitative and quantitative real-time PCR and RT-PCR.

Conditions

• Hematopietic Stem Cell Transplantation
• Blood Virome
• RNA Virus Infections
• DNA Virus Infections

Interventions

DIAGNOSTIC_TEST

Real time PCR and RT-PCR for DNA and RNA viral species

Qualitative and quantitative real time PCR and RT-PCR assays will be performed on hematopoietic stem cell transplant recipients' plasma samples collected at five specific time points over a one-year period after transplantation (the day of transplantation (D0), 30 days (D30), 3 months (M3), 6 months (M6) and one year (Y1) after transplantation), for the screening of a selection of at least 21 DNA and RNA viruses (belonging to the Herpesviridae, Polyomaviridae, Adenoviridae, Parvoviridae, Anelloviridae, Picornaviridae and Flaviviridae families).

Sponsors & Collaborators

• Prof Laurent Kaiser

  collaborator UNKNOWN

• Dr Samuel Cordey

  collaborator UNKNOWN

• Dr Stavroula Masouridi-Levrat

  collaborator UNKNOWN

• Prof Christian Van Delden

  collaborator UNKNOWN

• Prof Yves Chalandon

  collaborator UNKNOWN

• Dr Dionysios Neofytos

  collaborator UNKNOWN

• Dr Federico Simonetta

  collaborator UNKNOWN

• Dr Diem-Lan Vu Cantero

  collaborator UNKNOWN

• University Hospital, Geneva

  lead OTHER

Principal Investigators

• Marie-Céline Zanella · University Hospitals of Geneva

• Laurent Kaiser · University Hospitals of Geneva

Eligibility

Min Age

18 Years

Sex

ALL

Healthy Volunteers

No

Timeline & Regulatory

Start

2017-03-01

Primary Completion

2020-12-31

Completion

2020-12-31

Countries

• Switzerland

Study Locations