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Molecular Profiling to Improve Outcome of Patients in Cancer. A Pilot Study

NCT03546127 · Status: COMPLETED · Type: OBSERVATIONAL · Enrollment: 24

Last updated 2025-09-11

No results posted yet for this study

Summary

Next Generation Sequencing in cancer: a feasibility study in France to assess sample circuit and to perform analyzes within a limited time.

Conditions

  • Colorectal Carcinoma
  • Soft Tissue Sarcoma

Interventions

GENETIC

Next Generation Sequencing (NGS): exome, RNA seq

Tumor and blood samples will be sequenced at medium-high coverage at the whole genome (exome) and transcriptome levels (RNA Seq). This will allow detecting variants in a larger set of samples even though only from the main clone will be precisely measured. The whole exome will be performed at a mean coverage of at least 60x for the normal DNA samples and 120x for the tumor DNA samples. The transcriptome of the tumor will be performed at enough depth of coverage to detect gene fusions, transcriptome variants and measure the digital expression of already annotated isoforms. For both sequencing configurations: * Data from each cancer and normal genome will be analysed for the presence of somatic variants. * DNA and RNA sequencing results will be integrated. * Technical replication for the mutations / chromosome alterations / transcript fusions that most likely drive the tumour process will be performed via Target Resequencing of the genomic / coding regions of interest.

Sponsors & Collaborators

  • Institut Bergonié

    collaborator OTHER

  • Plateforme labellisée Inca - Institut Bergonié, Bordeaux

    collaborator UNKNOWN

  • Plateforme labellisée Inca - Hôpital Européen Georges Pompidou, Paris

    collaborator UNKNOWN

  • CNRGH, Evry

    collaborator UNKNOWN

  • EUCLID Clinical Trial Platform

    collaborator OTHER

  • Institut National de la Santé Et de la Recherche Médicale, France

    lead OTHER_GOV

Eligibility

Min Age
18 Years
Sex
ALL
Healthy Volunteers
No

Timeline & Regulatory

Start
2017-05-23
Primary Completion
2017-09-21
Completion
2017-09-21

Countries

  • France

Study Locations

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Read the full study record

This page highlights key information. For complete eligibility criteria, study locations, investigator contacts, and the full protocol, visit the original record on ClinicalTrials.gov.

View NCT03546127 on ClinicalTrials.gov