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Modeling Bronchial Epithelium Modifications Associated With COPD Using iPS

NCT03181204 · Status: COMPLETED · Type: OBSERVATIONAL · Enrollment: 11

Last updated 2023-09-08

No results posted yet for this study

Summary

The primary objective of this study is to to verify the feasibility of obtaining and comparing two epithelia in two populations based on the following experiments:

* Differentiation of an Induced Pluripotent Stem cell (iPS) clone derived from cutaneous biopsy in a population of heavy smokers (plus patients with chronic obstructive pulmonary disease) in order to obtain differentiated bronchial epithelia in vitro.
* For each of these same patients, generation of bronchial epithelium in vitro from bronchial biopsy using human bronchial epithelial cells (HBECs) in air-liquid interface (ALI) cultures.

Conditions

  • Pulmonary Disease, Chronic Obstructive
  • Smoking

Interventions

PROCEDURE

Bronchial biopsy

The bronchial biopsy is part of this study's inclusion criteria and is required during routine care for the patient. Bronchial fibroscopy is performed under general or local anesthesia. Biopsies are performed using forceps and according to current recommendations. One biopsy will be fixed immediately in RCL2 medium while the others (between 3 and 6) will be kept in isotonic saline and rapidly prepared for air-liquid-interface (ALI) cell culture.

PROCEDURE

Skin biopsy

The skin biopsy will be performed after local anesthesia by hypodermic injection of a 1% Xylocaine solution. A single sample of a skin fragment about 3 mm in diameter will be necessary.

PROCEDURE

Blood sample

The blood sample will be taken at the end of the cutaneous biospy, approximately 20 ml of blood will be collected.

Sponsors & Collaborators

  • University Hospital, Montpellier

    lead OTHER

Principal Investigators

  • Arnaud Bourdin, MD, PhD · University Hospital, Montpellier

Eligibility

Min Age
18 Years
Max Age
55 Years
Sex
ALL
Healthy Volunteers
No

Timeline & Regulatory

Start
2017-08-07
Primary Completion
2022-03-09
Completion
2022-03-09

Countries

  • France

Study Locations

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Read the full study record

This page highlights key information. For complete eligibility criteria, study locations, investigator contacts, and the full protocol, visit the original record on ClinicalTrials.gov.

View NCT03181204 on ClinicalTrials.gov