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Effect of Low Level Laser Therapy on Endometrial Cells: An In Vitro Study

NCT02764034 · Status: AVAILABLE · Type: EXPANDED_ACCESS

Last updated 2017-07-25

No results posted yet for this study

Summary

The human endometrium is an extremely sensitive target. LLLT can enhance the proliferation rate of various cell lines, it produces higher rates of ATP, RNA, and DNA synthesis in stem cells and other cell lines. Thus, LLLT improves the proliferation of the cells without causing any cytotoxic effects.

The aim of this work is to assess the ability of low level laser therapy in enhancing endometrial proliferation and increasing endometrial receptivity.

A number of 120 human endometrial samples will be studied, and will be collected from 40 infertile women attending the infertility clinic at NRC. Each human endometrial sample will be divided into 3 plates, in order to establish 3 main groups of 40 culture plates for single laser exposure group versus 40 culture plates for multiple laser exposure group and 40 tissue culture plates for control samples, thus a total of 120 tissue culture plates will be studied, the study groups will be exposed to low level laser therapy and compared to their matched controls.. Assessment of number and size of cells after LLLT as a marker of normal proliferation and b) The expression of Integrin aVB3 "alpha v B3", MUC-1 and LIFand the development of pinopodes on the surface of epithelial cells as markers of endometrial receptivity and the detection of PTEN tumor suppressor gene as a marker of abnormal proliferation or premalignant condition will be performed to assess the effect of LLLT on the endometrial cell culture. This study might offer a new therapeutic modality which might increase endometrial thickness and enhance receptivity .

Conditions

Interventions

DEVICE

Low level Laser diode machine

wavelength 635 Low level diode laser power control time control Continuous mode

Sponsors & Collaborators

  • Cairo University

    lead OTHER

Principal Investigators

  • Amr H. El Noury, MD, FRCOG · Cairo University

  • Marwa A. El Shaer, MD · Professor of Pathology at National Research Centre

  • Mona I. Morsy, MD · Lecturer in Molecular Biology in National Institute of Laser and Enhanced Science

  • Osama M. Azmy, MD, FRCOG · Professor of obstetrics and gynecology at National Research Centre

Eligibility

Min Age
20 Years
Max Age
38 Years
Sex
FEMALE
Healthy Volunteers
No

Timeline & Regulatory

Countries

  • Egypt

Study Locations

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Entities

Diseases

Read the full study record

This page highlights key information. For complete eligibility criteria, study locations, investigator contacts, and the full protocol, visit the original record on ClinicalTrials.gov.

View NCT02764034 on ClinicalTrials.gov