Muscle Oxygenation, Type 1 Diabetes, and Glycated Hemoglobin

Summary

Most of the studies concerning aerobic fitness in Type 1 diabetic patients noted a relationship between impaired aerobic fitness and high glycated haemoglobin (HbA1c) levels, reflecting poor long term glycaemic control. To explain this relationship, the indirect effect of chronically high blood glucose levels on cardiovascular complications - and hence on exercise cardiovascular adaptations - are often mentioned. However, one could wonder if HbA1c could also have a direct impact on aerobic fitness patients with Type 1 diabetes. Haemoglobin glycation may increase its O2 affinity, thus limiting the O2 availability at the muscular level and impairing maximal aerobic power. Moreover, chronic hyperglycaemia might have deleterious effect on muscle mitochondrial capacity to use O2. The aim of this study is to assess the effect of Type 1 diabetes and of HbA1c level on muscular oxygen delivery and use and hence on aerobic fitness.

Conditions

• Type 1 Diabetes

Interventions

BEHAVIORAL

Incremental maximal exercise

The exercise test starts 2-4h after a standardised breakfast. After a 2-min resting period sitting on the cycle ergometer (Excalibur Sport, Lode B.V, Medical Technology, Groningen, Netherlands), the test starts at 30 watts with a 20 watts increment every 2min until exhaustion.

DIETARY_SUPPLEMENT

Oral Glucose Tolerance Test

The subjects arrive after an overnight fast and have a 75g Glucose Oral Charge.

PROCEDURE

Muscle biopsy

A sample of vastus lateralis (less than 150mg) is taken with a specific needle under local anesthesia.

PROCEDURE

Combined DLCO-DLNO

Lung carbon monoxide and nitric oxide diffusion capacities are assessed at rest in a sitting position.

PROCEDURE

Dual energy X-ray absorptiometry

Body composition is measured using dual energy X-ray absorptiometry at rest.

PROCEDURE

Accelerometry over one week

The subjects wear an uniaxial accelerometer over one week to assess their usual physical activity level

OTHER

Questionnaires

Diet questionnaire, quality-of-life questionnaires, physical activity questionnaires

Sponsors & Collaborators

• University Hospital, Lille

  lead OTHER

Principal Investigators

• Elsa HEYMAN, PHD · EA4488 'Physical activity, Muscle, Health

• Pierre FONTAINE, MD-PHD · CHRU LILLE

Eligibility

Min Age

18 Years

Max Age

40 Years

Sex

ALL

Healthy Volunteers

Yes

Timeline & Regulatory

Start

2010-03-31

Primary Completion

2013-11-30

Completion

2013-12-31

Countries

• France

Study Locations