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Drug Transport in Patients With Anorexia Nervosa

NCT00176150 · Status: UNKNOWN · Type: OBSERVATIONAL

Last updated 2005-09-15

No results posted yet for this study

Summary

The aim of this study is to investigate whether patients with anorexia nervosa have elevated plasma cholesterol levels and, if elevated plasma cholesterol levels influence drug transport by the ABC-transporter P-gp (P-glycoprotein, MDR 1/ABCB1). We will isolate peripheral blood mononuclear cells (PBMCs) and total RNA from the blood of patients with anorexia nervosa and healthy subjects as control. PBMCs will be used for quantitative cholesterol determination and for measuring the activity of P-gp using a FACS (fluorescence-activated cell sorting) method (rhodamine123 efflux). Total RNA will be used for quantitative expression analysis of P-gp by reverse transcriptase real time PCR.

Conditions

  • Anorexia Nervosa

Sponsors & Collaborators

  • Heidelberg University

    lead OTHER

Principal Investigators

  • Walter Emil Haefeli, Prof. Dr. · Department of Internal Medicine VI, Clinical Pharmacology and Pharmacoepidemilogy

Eligibility

Min Age
15 Years
Max Age
30 Years
Sex
FEMALE
Healthy Volunteers
No

Timeline & Regulatory

Countries

  • Germany

Study Locations

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Read the full study record

This page highlights key information. For complete eligibility criteria, study locations, investigator contacts, and the full protocol, visit the original record on ClinicalTrials.gov.

View NCT00176150 on ClinicalTrials.gov