Trial Outcomes & Findings for Mechanistic Drivers of Acute PAPE Responsiveness: Muscle Architecture, Contractile Kinetics, and Excitability in a Randomized Controlled Trial (NCT NCT06982937)

NCT ID: NCT06982937

Last Updated: 2025-12-16

Results Overview

Recruitment status

COMPLETED

Study phase

NA

Target enrollment

44 participants

Primary outcome timeframe

6 minutes post-intervention

Results posted on

2025-12-16

Participant Flow

Participant milestones

Participant milestones
Measure
High-Intensity Half-Squat
Participants perform one set half-squats using a free-weight barbell in a squat rack to elicit PAPE High-Intensity Half-Squat: Participants in the conditioning arm perform a single set of two to three half-squats with a free-weight barbell set at 90% of their one-rep max (1RM). Each squat is taken down until the thigh is parallel to the floor (knee at \~90°) and then driven upward as explosively as possible.
Treadmill Running
Participants jogged on a level treadmill for five minutes at a comfortable pace to provide general muscle warm-up without significant neuromuscular priming. Treadmill running: Participants engaged in a standardized 5-minute treadmill warm-up. Participants were instructed to walk at a moderate, self-selected pace of approximately 7-9 km/h, maintaining an incline of 0%. Heart rate and perceived exertion were monitored to ensure that the activity remained within a light-intensity range (Borg scale: 6-11).
Overall Study
STARTED
22
22
Overall Study
COMPLETED
22
22
Overall Study
NOT COMPLETED
0
0

Reasons for withdrawal

Withdrawal data not reported

Baseline Characteristics

Mechanistic Drivers of Acute PAPE Responsiveness: Muscle Architecture, Contractile Kinetics, and Excitability in a Randomized Controlled Trial

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
High-Intensity Half-Squat
n=22 Participants
Participants perform one set half-squats using a free-weight barbell in a squat rack to elicit PAPE High-Intensity Half-Squat: Participants in the conditioning arm perform a single set of two to three half-squats with a free-weight barbell set at 90% of their one-rep max (1RM). Each squat is taken down until the thigh is parallel to the floor (knee at \~90°) and then driven upward as explosively as possible.
Treadmill Running
n=22 Participants
Participants jogged on a level treadmill for five minutes at a comfortable pace to provide general muscle warm-up without significant neuromuscular priming. Treadmill running: Participants engaged in a standardized 5-minute treadmill warm-up. Participants were instructed to walk at a moderate, self-selected pace of approximately 7-9 km/h, maintaining an incline of 0%. Heart rate and perceived exertion were monitored to ensure that the activity remained within a light-intensity range (Borg scale: 6-11).
Total
n=44 Participants
Total of all reporting groups
Race (NIH/OMB)
White
22 Participants
n=9 Participants
22 Participants
n=6 Participants
44 Participants
n=9 Participants
Race (NIH/OMB)
More than one race
0 Participants
n=9 Participants
0 Participants
n=6 Participants
0 Participants
n=9 Participants
Age, Categorical
<=18 years
0 Participants
n=9 Participants
0 Participants
n=6 Participants
0 Participants
n=9 Participants
Age, Categorical
Between 18 and 65 years
22 Participants
n=9 Participants
22 Participants
n=6 Participants
44 Participants
n=9 Participants
Age, Categorical
>=65 years
0 Participants
n=9 Participants
0 Participants
n=6 Participants
0 Participants
n=9 Participants
Sex: Female, Male
Female
0 Participants
n=9 Participants
0 Participants
n=6 Participants
0 Participants
n=9 Participants
Sex: Female, Male
Male
22 Participants
n=9 Participants
22 Participants
n=6 Participants
44 Participants
n=9 Participants
Race (NIH/OMB)
American Indian or Alaska Native
0 Participants
n=9 Participants
0 Participants
n=6 Participants
0 Participants
n=9 Participants
Race (NIH/OMB)
Asian
0 Participants
n=9 Participants
0 Participants
n=6 Participants
0 Participants
n=9 Participants
Race (NIH/OMB)
Native Hawaiian or Other Pacific Islander
0 Participants
n=9 Participants
0 Participants
n=6 Participants
0 Participants
n=9 Participants
Race (NIH/OMB)
Black or African American
0 Participants
n=9 Participants
0 Participants
n=6 Participants
0 Participants
n=9 Participants
Race (NIH/OMB)
Unknown or Not Reported
0 Participants
n=9 Participants
0 Participants
n=6 Participants
0 Participants
n=9 Participants
Region of Enrollment
Poland
22 participants
n=9 Participants
22 participants
n=6 Participants
44 participants
n=9 Participants
%MHC-1
26.14 %
STANDARD_DEVIATION 9.19 • n=9 Participants
27.28 %
STANDARD_DEVIATION 10.01 • n=6 Participants
26.71 %
STANDARD_DEVIATION 9.6 • n=9 Participants
Contraction time
26.34 ms
STANDARD_DEVIATION 2.15 • n=9 Participants
23.01 ms
STANDARD_DEVIATION 1.08 • n=6 Participants
24.68 ms
STANDARD_DEVIATION 0.76 • n=9 Participants
Delay time
23.47 ms
STANDARD_DEVIATION 1.16 • n=9 Participants
21.91 ms
STANDARD_DEVIATION 1.14 • n=6 Participants
22.69 ms
STANDARD_DEVIATION 0.02 • n=9 Participants
Muscle displacement
6.78 mm
STANDARD_DEVIATION 1.93 • n=9 Participants
6.61 mm
STANDARD_DEVIATION 1.59 • n=6 Participants
6.70 mm
STANDARD_DEVIATION 1.75 • n=9 Participants
Relaxation time
18.25 ms
STANDARD_DEVIATION 5.16 • n=9 Participants
16.61 ms
STANDARD_DEVIATION 3.82 • n=6 Participants
17.44 ms
STANDARD_DEVIATION 4.56 • n=9 Participants
Sustain time
40.37 ms
STANDARD_DEVIATION 4.27 • n=9 Participants
37.49 ms
STANDARD_DEVIATION 4.13 • n=6 Participants
38.94 ms
STANDARD_DEVIATION 4.40 • n=9 Participants
Muscle thickness
2.47 cm
STANDARD_DEVIATION 0.36 • n=9 Participants
2.57 cm
STANDARD_DEVIATION 0.28 • n=6 Participants
2.53 cm
STANDARD_DEVIATION 0.32 • n=9 Participants
Pennation angle
13.76 degrees
STANDARD_DEVIATION 2.05 • n=9 Participants
14.23 degrees
STANDARD_DEVIATION 1.75 • n=6 Participants
14.00 degrees
STANDARD_DEVIATION 1.90 • n=9 Participants
Fascile lenght
10.61 cm
STANDARD_DEVIATION 2.02 • n=9 Participants
10.67 cm
STANDARD_DEVIATION 2.12 • n=6 Participants
10.64 cm
STANDARD_DEVIATION 2.04 • n=9 Participants
Maximal strength level
98.07 kg
STANDARD_DEVIATION 9.63 • n=9 Participants
102.04 kg
STANDARD_DEVIATION 16.14 • n=6 Participants
100.06 kg
STANDARD_DEVIATION 13.29 • n=9 Participants
Jump height
45.23 cm
STANDARD_DEVIATION 4.95 • n=9 Participants
47.52 cm
STANDARD_DEVIATION 4.70 • n=6 Participants
46.38 cm
STANDARD_DEVIATION 4.91 • n=9 Participants
Muscle activation during countermovement jump
45.87 percentage of MVC
STANDARD_DEVIATION 12.78 • n=9 Participants
44.16 percentage of MVC
STANDARD_DEVIATION 10.92 • n=6 Participants
45.02 percentage of MVC
STANDARD_DEVIATION 11.78 • n=9 Participants

PRIMARY outcome

Timeframe: 4 minutes post-intervention

Outcome measures

Outcome measures
Measure
Treadmill Running
n=22 Participants
Participants jogged on a level treadmill for five minutes at a comfortable pace to provide general muscle warm-up without significant neuromuscular priming. Treadmill running: Participants engaged in a standardized 5-minute treadmill warm-up. Participants were instructed to walk at a moderate, self-selected pace of approximately 7-9 km/h, maintaining an incline of 0%. Heart rate and perceived exertion were monitored to ensure that the activity remained within a light-intensity range (Borg scale: 6-11).
High-Intensity Half-Squat
n=22 Participants
Participants perform one set half-squats using a free-weight barbell in a squat rack to elicit PAPE High-Intensity Half-Squat: Participants in the conditioning arm perform a single set of two to three half-squats with a free-weight barbell set at 90% of their one-rep max (1RM). Each squat is taken down until the thigh is parallel to the floor (knee at \~90°) and then driven upward as explosively as possible.
Jump Height 4 Minute
47.41 cm
Standard Deviation 5.13
45.34 cm
Standard Deviation 5.28

PRIMARY outcome

Timeframe: 6 minutes post-intervention

Outcome measures

Outcome measures
Measure
Treadmill Running
n=22 Participants
Participants jogged on a level treadmill for five minutes at a comfortable pace to provide general muscle warm-up without significant neuromuscular priming. Treadmill running: Participants engaged in a standardized 5-minute treadmill warm-up. Participants were instructed to walk at a moderate, self-selected pace of approximately 7-9 km/h, maintaining an incline of 0%. Heart rate and perceived exertion were monitored to ensure that the activity remained within a light-intensity range (Borg scale: 6-11).
High-Intensity Half-Squat
n=22 Participants
Participants perform one set half-squats using a free-weight barbell in a squat rack to elicit PAPE High-Intensity Half-Squat: Participants in the conditioning arm perform a single set of two to three half-squats with a free-weight barbell set at 90% of their one-rep max (1RM). Each squat is taken down until the thigh is parallel to the floor (knee at \~90°) and then driven upward as explosively as possible.
Jump Height 6 Minute
47.41 cm
Standard Deviation 3.92
48.01 cm
Standard Deviation 5.13

PRIMARY outcome

Timeframe: 8 minutes post-intervention

Outcome measures

Outcome measures
Measure
Treadmill Running
n=22 Participants
Participants jogged on a level treadmill for five minutes at a comfortable pace to provide general muscle warm-up without significant neuromuscular priming. Treadmill running: Participants engaged in a standardized 5-minute treadmill warm-up. Participants were instructed to walk at a moderate, self-selected pace of approximately 7-9 km/h, maintaining an incline of 0%. Heart rate and perceived exertion were monitored to ensure that the activity remained within a light-intensity range (Borg scale: 6-11).
High-Intensity Half-Squat
n=22 Participants
Participants perform one set half-squats using a free-weight barbell in a squat rack to elicit PAPE High-Intensity Half-Squat: Participants in the conditioning arm perform a single set of two to three half-squats with a free-weight barbell set at 90% of their one-rep max (1RM). Each squat is taken down until the thigh is parallel to the floor (knee at \~90°) and then driven upward as explosively as possible.
Jump Height 8 Minute
48.10 cm
Standard Deviation 4.60
47.08 cm
Standard Deviation 5.31

PRIMARY outcome

Timeframe: 10 minutes post-intervention

Outcome measures

Outcome measures
Measure
Treadmill Running
n=22 Participants
Participants jogged on a level treadmill for five minutes at a comfortable pace to provide general muscle warm-up without significant neuromuscular priming. Treadmill running: Participants engaged in a standardized 5-minute treadmill warm-up. Participants were instructed to walk at a moderate, self-selected pace of approximately 7-9 km/h, maintaining an incline of 0%. Heart rate and perceived exertion were monitored to ensure that the activity remained within a light-intensity range (Borg scale: 6-11).
High-Intensity Half-Squat
n=22 Participants
Participants perform one set half-squats using a free-weight barbell in a squat rack to elicit PAPE High-Intensity Half-Squat: Participants in the conditioning arm perform a single set of two to three half-squats with a free-weight barbell set at 90% of their one-rep max (1RM). Each squat is taken down until the thigh is parallel to the floor (knee at \~90°) and then driven upward as explosively as possible.
Jump Height 10 Minute
47.59 cm
Standard Deviation 3.90
46.17 cm
Standard Deviation 5.17

PRIMARY outcome

Timeframe: 12 minute post-intervention

Outcome measures

Outcome measures
Measure
Treadmill Running
n=22 Participants
Participants jogged on a level treadmill for five minutes at a comfortable pace to provide general muscle warm-up without significant neuromuscular priming. Treadmill running: Participants engaged in a standardized 5-minute treadmill warm-up. Participants were instructed to walk at a moderate, self-selected pace of approximately 7-9 km/h, maintaining an incline of 0%. Heart rate and perceived exertion were monitored to ensure that the activity remained within a light-intensity range (Borg scale: 6-11).
High-Intensity Half-Squat
n=22 Participants
Participants perform one set half-squats using a free-weight barbell in a squat rack to elicit PAPE High-Intensity Half-Squat: Participants in the conditioning arm perform a single set of two to three half-squats with a free-weight barbell set at 90% of their one-rep max (1RM). Each squat is taken down until the thigh is parallel to the floor (knee at \~90°) and then driven upward as explosively as possible.
Jump Height 12 Minute
47.91 cm
Standard Deviation 4.27
45.27 cm
Standard Deviation 5.14

PRIMARY outcome

Timeframe: Immediate post-intervention

Outcome measures

Outcome measures
Measure
Treadmill Running
n=22 Participants
Participants jogged on a level treadmill for five minutes at a comfortable pace to provide general muscle warm-up without significant neuromuscular priming. Treadmill running: Participants engaged in a standardized 5-minute treadmill warm-up. Participants were instructed to walk at a moderate, self-selected pace of approximately 7-9 km/h, maintaining an incline of 0%. Heart rate and perceived exertion were monitored to ensure that the activity remained within a light-intensity range (Borg scale: 6-11).
High-Intensity Half-Squat
n=22 Participants
Participants perform one set half-squats using a free-weight barbell in a squat rack to elicit PAPE High-Intensity Half-Squat: Participants in the conditioning arm perform a single set of two to three half-squats with a free-weight barbell set at 90% of their one-rep max (1RM). Each squat is taken down until the thigh is parallel to the floor (knee at \~90°) and then driven upward as explosively as possible.
Muscle Thickness Post
2.63 cm
Standard Deviation 0.33
2.95 cm
Standard Deviation 0.37

PRIMARY outcome

Timeframe: 4 minute post-intervention (single session)

Outcome measures

Outcome measures
Measure
Treadmill Running
n=22 Participants
Participants jogged on a level treadmill for five minutes at a comfortable pace to provide general muscle warm-up without significant neuromuscular priming. Treadmill running: Participants engaged in a standardized 5-minute treadmill warm-up. Participants were instructed to walk at a moderate, self-selected pace of approximately 7-9 km/h, maintaining an incline of 0%. Heart rate and perceived exertion were monitored to ensure that the activity remained within a light-intensity range (Borg scale: 6-11).
High-Intensity Half-Squat
n=22 Participants
Participants perform one set half-squats using a free-weight barbell in a squat rack to elicit PAPE High-Intensity Half-Squat: Participants in the conditioning arm perform a single set of two to three half-squats with a free-weight barbell set at 90% of their one-rep max (1RM). Each squat is taken down until the thigh is parallel to the floor (knee at \~90°) and then driven upward as explosively as possible.
Muscle Thickness 4 Minute
2.58 cm
Standard Deviation 0.33
2.92 cm
Standard Deviation 0.35

PRIMARY outcome

Timeframe: 6 minutes post-intervention

Outcome measures

Outcome measures
Measure
Treadmill Running
n=22 Participants
Participants jogged on a level treadmill for five minutes at a comfortable pace to provide general muscle warm-up without significant neuromuscular priming. Treadmill running: Participants engaged in a standardized 5-minute treadmill warm-up. Participants were instructed to walk at a moderate, self-selected pace of approximately 7-9 km/h, maintaining an incline of 0%. Heart rate and perceived exertion were monitored to ensure that the activity remained within a light-intensity range (Borg scale: 6-11).
High-Intensity Half-Squat
n=22 Participants
Participants perform one set half-squats using a free-weight barbell in a squat rack to elicit PAPE High-Intensity Half-Squat: Participants in the conditioning arm perform a single set of two to three half-squats with a free-weight barbell set at 90% of their one-rep max (1RM). Each squat is taken down until the thigh is parallel to the floor (knee at \~90°) and then driven upward as explosively as possible.
Muscle Thickness 6 Minute
2.58 cm
Standard Deviation 0.30
2.83 cm
Standard Deviation 0.37

PRIMARY outcome

Timeframe: 8 minutes post-intervention

Outcome measures

Outcome measures
Measure
Treadmill Running
n=22 Participants
Participants jogged on a level treadmill for five minutes at a comfortable pace to provide general muscle warm-up without significant neuromuscular priming. Treadmill running: Participants engaged in a standardized 5-minute treadmill warm-up. Participants were instructed to walk at a moderate, self-selected pace of approximately 7-9 km/h, maintaining an incline of 0%. Heart rate and perceived exertion were monitored to ensure that the activity remained within a light-intensity range (Borg scale: 6-11).
High-Intensity Half-Squat
n=22 Participants
Participants perform one set half-squats using a free-weight barbell in a squat rack to elicit PAPE High-Intensity Half-Squat: Participants in the conditioning arm perform a single set of two to three half-squats with a free-weight barbell set at 90% of their one-rep max (1RM). Each squat is taken down until the thigh is parallel to the floor (knee at \~90°) and then driven upward as explosively as possible.
Muscle Thickness 8 Minute
2.53 cm
Standard Deviation 0.32
2.70 cm
Standard Deviation 0.36

PRIMARY outcome

Timeframe: 10 minutes post-intervention

Outcome measures

Outcome measures
Measure
Treadmill Running
n=22 Participants
Participants jogged on a level treadmill for five minutes at a comfortable pace to provide general muscle warm-up without significant neuromuscular priming. Treadmill running: Participants engaged in a standardized 5-minute treadmill warm-up. Participants were instructed to walk at a moderate, self-selected pace of approximately 7-9 km/h, maintaining an incline of 0%. Heart rate and perceived exertion were monitored to ensure that the activity remained within a light-intensity range (Borg scale: 6-11).
High-Intensity Half-Squat
n=22 Participants
Participants perform one set half-squats using a free-weight barbell in a squat rack to elicit PAPE High-Intensity Half-Squat: Participants in the conditioning arm perform a single set of two to three half-squats with a free-weight barbell set at 90% of their one-rep max (1RM). Each squat is taken down until the thigh is parallel to the floor (knee at \~90°) and then driven upward as explosively as possible.
Muscle Thickness 10 Minute
2.60 cm
Standard Deviation 0.39
2.66 cm
Standard Deviation 0.35

PRIMARY outcome

Timeframe: 12 minutes post-intervention

Outcome measures

Outcome measures
Measure
Treadmill Running
n=22 Participants
Participants jogged on a level treadmill for five minutes at a comfortable pace to provide general muscle warm-up without significant neuromuscular priming. Treadmill running: Participants engaged in a standardized 5-minute treadmill warm-up. Participants were instructed to walk at a moderate, self-selected pace of approximately 7-9 km/h, maintaining an incline of 0%. Heart rate and perceived exertion were monitored to ensure that the activity remained within a light-intensity range (Borg scale: 6-11).
High-Intensity Half-Squat
n=22 Participants
Participants perform one set half-squats using a free-weight barbell in a squat rack to elicit PAPE High-Intensity Half-Squat: Participants in the conditioning arm perform a single set of two to three half-squats with a free-weight barbell set at 90% of their one-rep max (1RM). Each squat is taken down until the thigh is parallel to the floor (knee at \~90°) and then driven upward as explosively as possible.
Muscle Thickness 12 Minute
2.55 cm
Standard Deviation 0.38
2.57 cm
Standard Deviation 0.44

PRIMARY outcome

Timeframe: Immediate post-intervention

Outcome measures

Outcome measures
Measure
Treadmill Running
n=22 Participants
Participants jogged on a level treadmill for five minutes at a comfortable pace to provide general muscle warm-up without significant neuromuscular priming. Treadmill running: Participants engaged in a standardized 5-minute treadmill warm-up. Participants were instructed to walk at a moderate, self-selected pace of approximately 7-9 km/h, maintaining an incline of 0%. Heart rate and perceived exertion were monitored to ensure that the activity remained within a light-intensity range (Borg scale: 6-11).
High-Intensity Half-Squat
n=22 Participants
Participants perform one set half-squats using a free-weight barbell in a squat rack to elicit PAPE High-Intensity Half-Squat: Participants in the conditioning arm perform a single set of two to three half-squats with a free-weight barbell set at 90% of their one-rep max (1RM). Each squat is taken down until the thigh is parallel to the floor (knee at \~90°) and then driven upward as explosively as possible.
Pennation Angle Post
13.84 degrees
Standard Deviation 1.98
17.83 degrees
Standard Deviation 2.49

PRIMARY outcome

Timeframe: 4 minutes post-intervention

Outcome measures

Outcome measures
Measure
Treadmill Running
n=22 Participants
Participants jogged on a level treadmill for five minutes at a comfortable pace to provide general muscle warm-up without significant neuromuscular priming. Treadmill running: Participants engaged in a standardized 5-minute treadmill warm-up. Participants were instructed to walk at a moderate, self-selected pace of approximately 7-9 km/h, maintaining an incline of 0%. Heart rate and perceived exertion were monitored to ensure that the activity remained within a light-intensity range (Borg scale: 6-11).
High-Intensity Half-Squat
n=22 Participants
Participants perform one set half-squats using a free-weight barbell in a squat rack to elicit PAPE High-Intensity Half-Squat: Participants in the conditioning arm perform a single set of two to three half-squats with a free-weight barbell set at 90% of their one-rep max (1RM). Each squat is taken down until the thigh is parallel to the floor (knee at \~90°) and then driven upward as explosively as possible.
Pennation Angle 4 Minute
13.37 degress
Standard Deviation 2.07
16.95 degress
Standard Deviation 2.63

PRIMARY outcome

Timeframe: 6 minutes post-intervention

Outcome measures

Outcome measures
Measure
Treadmill Running
n=22 Participants
Participants jogged on a level treadmill for five minutes at a comfortable pace to provide general muscle warm-up without significant neuromuscular priming. Treadmill running: Participants engaged in a standardized 5-minute treadmill warm-up. Participants were instructed to walk at a moderate, self-selected pace of approximately 7-9 km/h, maintaining an incline of 0%. Heart rate and perceived exertion were monitored to ensure that the activity remained within a light-intensity range (Borg scale: 6-11).
High-Intensity Half-Squat
n=22 Participants
Participants perform one set half-squats using a free-weight barbell in a squat rack to elicit PAPE High-Intensity Half-Squat: Participants in the conditioning arm perform a single set of two to three half-squats with a free-weight barbell set at 90% of their one-rep max (1RM). Each squat is taken down until the thigh is parallel to the floor (knee at \~90°) and then driven upward as explosively as possible.
Pennation Angle 6 Minute
14.06 degrees
Standard Deviation 2.39
17.09 degrees
Standard Deviation 2.70

PRIMARY outcome

Timeframe: 8 minutes post-intervention

Outcome measures

Outcome measures
Measure
Treadmill Running
n=22 Participants
Participants jogged on a level treadmill for five minutes at a comfortable pace to provide general muscle warm-up without significant neuromuscular priming. Treadmill running: Participants engaged in a standardized 5-minute treadmill warm-up. Participants were instructed to walk at a moderate, self-selected pace of approximately 7-9 km/h, maintaining an incline of 0%. Heart rate and perceived exertion were monitored to ensure that the activity remained within a light-intensity range (Borg scale: 6-11).
High-Intensity Half-Squat
n=22 Participants
Participants perform one set half-squats using a free-weight barbell in a squat rack to elicit PAPE High-Intensity Half-Squat: Participants in the conditioning arm perform a single set of two to three half-squats with a free-weight barbell set at 90% of their one-rep max (1RM). Each squat is taken down until the thigh is parallel to the floor (knee at \~90°) and then driven upward as explosively as possible.
Pennation Angle 8 Minute
14.09 degrees
Standard Deviation 2.45
16.84 degrees
Standard Deviation 2.88

PRIMARY outcome

Timeframe: 10 minutes post-intervention

Outcome measures

Outcome measures
Measure
Treadmill Running
n=22 Participants
Participants jogged on a level treadmill for five minutes at a comfortable pace to provide general muscle warm-up without significant neuromuscular priming. Treadmill running: Participants engaged in a standardized 5-minute treadmill warm-up. Participants were instructed to walk at a moderate, self-selected pace of approximately 7-9 km/h, maintaining an incline of 0%. Heart rate and perceived exertion were monitored to ensure that the activity remained within a light-intensity range (Borg scale: 6-11).
High-Intensity Half-Squat
n=22 Participants
Participants perform one set half-squats using a free-weight barbell in a squat rack to elicit PAPE High-Intensity Half-Squat: Participants in the conditioning arm perform a single set of two to three half-squats with a free-weight barbell set at 90% of their one-rep max (1RM). Each squat is taken down until the thigh is parallel to the floor (knee at \~90°) and then driven upward as explosively as possible.
Pennation Angle 10 Minute
13.96 degrees
Standard Deviation 2.42
16.52 degrees
Standard Deviation 2.70

PRIMARY outcome

Timeframe: 12 minutes post-intervention

Outcome measures

Outcome measures
Measure
Treadmill Running
n=22 Participants
Participants jogged on a level treadmill for five minutes at a comfortable pace to provide general muscle warm-up without significant neuromuscular priming. Treadmill running: Participants engaged in a standardized 5-minute treadmill warm-up. Participants were instructed to walk at a moderate, self-selected pace of approximately 7-9 km/h, maintaining an incline of 0%. Heart rate and perceived exertion were monitored to ensure that the activity remained within a light-intensity range (Borg scale: 6-11).
High-Intensity Half-Squat
n=22 Participants
Participants perform one set half-squats using a free-weight barbell in a squat rack to elicit PAPE High-Intensity Half-Squat: Participants in the conditioning arm perform a single set of two to three half-squats with a free-weight barbell set at 90% of their one-rep max (1RM). Each squat is taken down until the thigh is parallel to the floor (knee at \~90°) and then driven upward as explosively as possible.
Pennation Angle 12 Minute
13.55 degrees
Standard Deviation 2.62
14.61 degrees
Standard Deviation 3.16

PRIMARY outcome

Timeframe: Immediate post-intervention

Outcome measures

Outcome measures
Measure
Treadmill Running
n=22 Participants
Participants jogged on a level treadmill for five minutes at a comfortable pace to provide general muscle warm-up without significant neuromuscular priming. Treadmill running: Participants engaged in a standardized 5-minute treadmill warm-up. Participants were instructed to walk at a moderate, self-selected pace of approximately 7-9 km/h, maintaining an incline of 0%. Heart rate and perceived exertion were monitored to ensure that the activity remained within a light-intensity range (Borg scale: 6-11).
High-Intensity Half-Squat
n=22 Participants
Participants perform one set half-squats using a free-weight barbell in a squat rack to elicit PAPE High-Intensity Half-Squat: Participants in the conditioning arm perform a single set of two to three half-squats with a free-weight barbell set at 90% of their one-rep max (1RM). Each squat is taken down until the thigh is parallel to the floor (knee at \~90°) and then driven upward as explosively as possible.
Fascicle Length Post
11.26 cm
Standard Deviation 2.49
9.82 cm
Standard Deviation 1.88

PRIMARY outcome

Timeframe: 4 minutes post-intervention

Outcome measures

Outcome measures
Measure
Treadmill Running
n=22 Participants
Participants jogged on a level treadmill for five minutes at a comfortable pace to provide general muscle warm-up without significant neuromuscular priming. Treadmill running: Participants engaged in a standardized 5-minute treadmill warm-up. Participants were instructed to walk at a moderate, self-selected pace of approximately 7-9 km/h, maintaining an incline of 0%. Heart rate and perceived exertion were monitored to ensure that the activity remained within a light-intensity range (Borg scale: 6-11).
High-Intensity Half-Squat
n=22 Participants
Participants perform one set half-squats using a free-weight barbell in a squat rack to elicit PAPE High-Intensity Half-Squat: Participants in the conditioning arm perform a single set of two to three half-squats with a free-weight barbell set at 90% of their one-rep max (1RM). Each squat is taken down until the thigh is parallel to the floor (knee at \~90°) and then driven upward as explosively as possible.
Fascicle Length 4 Minute
11.54 cm
Standard Deviation 3.17
10.27 cm
Standard Deviation 2.18

PRIMARY outcome

Timeframe: 6 minutes post-intervention

Outcome measures

Outcome measures
Measure
Treadmill Running
n=22 Participants
Participants jogged on a level treadmill for five minutes at a comfortable pace to provide general muscle warm-up without significant neuromuscular priming. Treadmill running: Participants engaged in a standardized 5-minute treadmill warm-up. Participants were instructed to walk at a moderate, self-selected pace of approximately 7-9 km/h, maintaining an incline of 0%. Heart rate and perceived exertion were monitored to ensure that the activity remained within a light-intensity range (Borg scale: 6-11).
High-Intensity Half-Squat
n=22 Participants
Participants perform one set half-squats using a free-weight barbell in a squat rack to elicit PAPE High-Intensity Half-Squat: Participants in the conditioning arm perform a single set of two to three half-squats with a free-weight barbell set at 90% of their one-rep max (1RM). Each squat is taken down until the thigh is parallel to the floor (knee at \~90°) and then driven upward as explosively as possible.
Fascicle Length 6 Minute
10.86 cm
Standard Deviation 1.90
9.80 cm
Standard Deviation 1.64

PRIMARY outcome

Timeframe: 8 minutes post-intervention

Outcome measures

Outcome measures
Measure
Treadmill Running
n=22 Participants
Participants jogged on a level treadmill for five minutes at a comfortable pace to provide general muscle warm-up without significant neuromuscular priming. Treadmill running: Participants engaged in a standardized 5-minute treadmill warm-up. Participants were instructed to walk at a moderate, self-selected pace of approximately 7-9 km/h, maintaining an incline of 0%. Heart rate and perceived exertion were monitored to ensure that the activity remained within a light-intensity range (Borg scale: 6-11).
High-Intensity Half-Squat
n=22 Participants
Participants perform one set half-squats using a free-weight barbell in a squat rack to elicit PAPE High-Intensity Half-Squat: Participants in the conditioning arm perform a single set of two to three half-squats with a free-weight barbell set at 90% of their one-rep max (1RM). Each squat is taken down until the thigh is parallel to the floor (knee at \~90°) and then driven upward as explosively as possible.
Fascicle Length 8 Minute
10.57 cm
Standard Deviation 1.60
9.59 cm
Standard Deviation 2.61

PRIMARY outcome

Timeframe: 10' minutes post-intervention

Outcome measures

Outcome measures
Measure
Treadmill Running
n=22 Participants
Participants jogged on a level treadmill for five minutes at a comfortable pace to provide general muscle warm-up without significant neuromuscular priming. Treadmill running: Participants engaged in a standardized 5-minute treadmill warm-up. Participants were instructed to walk at a moderate, self-selected pace of approximately 7-9 km/h, maintaining an incline of 0%. Heart rate and perceived exertion were monitored to ensure that the activity remained within a light-intensity range (Borg scale: 6-11).
High-Intensity Half-Squat
n=22 Participants
Participants perform one set half-squats using a free-weight barbell in a squat rack to elicit PAPE High-Intensity Half-Squat: Participants in the conditioning arm perform a single set of two to three half-squats with a free-weight barbell set at 90% of their one-rep max (1RM). Each squat is taken down until the thigh is parallel to the floor (knee at \~90°) and then driven upward as explosively as possible.
Fascicle Length 10 Minute
10.97 cm
Standard Deviation 1.76
9.69 cm
Standard Deviation 2.46

PRIMARY outcome

Timeframe: 12 minutes post-intervention

Outcome measures

Outcome measures
Measure
Treadmill Running
n=22 Participants
Participants jogged on a level treadmill for five minutes at a comfortable pace to provide general muscle warm-up without significant neuromuscular priming. Treadmill running: Participants engaged in a standardized 5-minute treadmill warm-up. Participants were instructed to walk at a moderate, self-selected pace of approximately 7-9 km/h, maintaining an incline of 0%. Heart rate and perceived exertion were monitored to ensure that the activity remained within a light-intensity range (Borg scale: 6-11).
High-Intensity Half-Squat
n=22 Participants
Participants perform one set half-squats using a free-weight barbell in a squat rack to elicit PAPE High-Intensity Half-Squat: Participants in the conditioning arm perform a single set of two to three half-squats with a free-weight barbell set at 90% of their one-rep max (1RM). Each squat is taken down until the thigh is parallel to the floor (knee at \~90°) and then driven upward as explosively as possible.
Fascicle Length 12 Minute
11.14 cm
Standard Deviation 1.98
10.69 cm
Standard Deviation 3.11

PRIMARY outcome

Timeframe: 4 minutes post-intervention

Surface electromyographic activity (voluntary activation level) of the vastus lateralis muscle recorded during a standardized countermovement jump. Higher values indicate greater muscle activation of the vastus lateralis during the jump, whereas lower values indicate reduced activation. Results presented as % from maximal voluntary contraction (MVC) during the procedure of normalization (locked at 90 degrees knee extension)

Outcome measures

Outcome measures
Measure
Treadmill Running
n=22 Participants
Participants jogged on a level treadmill for five minutes at a comfortable pace to provide general muscle warm-up without significant neuromuscular priming. Treadmill running: Participants engaged in a standardized 5-minute treadmill warm-up. Participants were instructed to walk at a moderate, self-selected pace of approximately 7-9 km/h, maintaining an incline of 0%. Heart rate and perceived exertion were monitored to ensure that the activity remained within a light-intensity range (Borg scale: 6-11).
High-Intensity Half-Squat
n=22 Participants
Participants perform one set half-squats using a free-weight barbell in a squat rack to elicit PAPE High-Intensity Half-Squat: Participants in the conditioning arm perform a single set of two to three half-squats with a free-weight barbell set at 90% of their one-rep max (1RM). Each squat is taken down until the thigh is parallel to the floor (knee at \~90°) and then driven upward as explosively as possible.
Muscle Activation During Countermovement Jump in 4 Minute
43.96 percentage of MVC
Standard Deviation 11.31
50.63 percentage of MVC
Standard Deviation 14.18

PRIMARY outcome

Timeframe: 6 minutes post-intervention

Surface electromyographic activity (voluntary activation level) of the vastus lateralis muscle recorded during a standardized countermovement jump. Higher values indicate greater muscle activation of the vastus lateralis during the jump, whereas lower values indicate reduced activation. Results presented as % from maximal voluntary contraction (MVC) during the procedure of normalization (locked at 90 degrees knee extension)

Outcome measures

Outcome measures
Measure
Treadmill Running
n=22 Participants
Participants jogged on a level treadmill for five minutes at a comfortable pace to provide general muscle warm-up without significant neuromuscular priming. Treadmill running: Participants engaged in a standardized 5-minute treadmill warm-up. Participants were instructed to walk at a moderate, self-selected pace of approximately 7-9 km/h, maintaining an incline of 0%. Heart rate and perceived exertion were monitored to ensure that the activity remained within a light-intensity range (Borg scale: 6-11).
High-Intensity Half-Squat
n=22 Participants
Participants perform one set half-squats using a free-weight barbell in a squat rack to elicit PAPE High-Intensity Half-Squat: Participants in the conditioning arm perform a single set of two to three half-squats with a free-weight barbell set at 90% of their one-rep max (1RM). Each squat is taken down until the thigh is parallel to the floor (knee at \~90°) and then driven upward as explosively as possible.
Muscle Activation During Countermovement Jump in 6 Minute
43.96 percentage of MVC
Standard Deviation 11.31
45.29 percentage of MVC
Standard Deviation 13.85

PRIMARY outcome

Timeframe: 8 minutes post-intervention

Surface electromyographic activity (voluntary activation level) of the vastus lateralis muscle recorded during a standardized countermovement jump. Higher values indicate greater muscle activation of the vastus lateralis during the jump, whereas lower values indicate reduced activation. Results presented as % from maximal voluntary contraction (MVC) during the procedure of normalization (locked at 90 degrees knee extension)

Outcome measures

Outcome measures
Measure
Treadmill Running
n=22 Participants
Participants jogged on a level treadmill for five minutes at a comfortable pace to provide general muscle warm-up without significant neuromuscular priming. Treadmill running: Participants engaged in a standardized 5-minute treadmill warm-up. Participants were instructed to walk at a moderate, self-selected pace of approximately 7-9 km/h, maintaining an incline of 0%. Heart rate and perceived exertion were monitored to ensure that the activity remained within a light-intensity range (Borg scale: 6-11).
High-Intensity Half-Squat
n=22 Participants
Participants perform one set half-squats using a free-weight barbell in a squat rack to elicit PAPE High-Intensity Half-Squat: Participants in the conditioning arm perform a single set of two to three half-squats with a free-weight barbell set at 90% of their one-rep max (1RM). Each squat is taken down until the thigh is parallel to the floor (knee at \~90°) and then driven upward as explosively as possible.
Muscle Activation During Countermovement Jump in 8 Minute
49.43 percentage of MVC
Standard Deviation 12.44
46.38 percentage of MVC
Standard Deviation 13.61

PRIMARY outcome

Timeframe: 10 minutes post-intervention

Surface electromyographic activity (voluntary activation level) of the vastus lateralis muscle recorded during a standardized countermovement jump. Higher values indicate greater muscle activation of the vastus lateralis during the jump, whereas lower values indicate reduced activation. Results presented as % from maximal voluntary contraction (MVC) during the procedure of normalization (locked at 90 degrees knee extension)

Outcome measures

Outcome measures
Measure
Treadmill Running
n=22 Participants
Participants jogged on a level treadmill for five minutes at a comfortable pace to provide general muscle warm-up without significant neuromuscular priming. Treadmill running: Participants engaged in a standardized 5-minute treadmill warm-up. Participants were instructed to walk at a moderate, self-selected pace of approximately 7-9 km/h, maintaining an incline of 0%. Heart rate and perceived exertion were monitored to ensure that the activity remained within a light-intensity range (Borg scale: 6-11).
High-Intensity Half-Squat
n=22 Participants
Participants perform one set half-squats using a free-weight barbell in a squat rack to elicit PAPE High-Intensity Half-Squat: Participants in the conditioning arm perform a single set of two to three half-squats with a free-weight barbell set at 90% of their one-rep max (1RM). Each squat is taken down until the thigh is parallel to the floor (knee at \~90°) and then driven upward as explosively as possible.
Muscle Activation During Countermovement Jump in 10 Minute
51.71 percentage of MVC
Standard Deviation 14.85
48.76 percentage of MVC
Standard Deviation 21.90

PRIMARY outcome

Timeframe: 12 minutes post-intervention

Surface electromyographic activity (voluntary activation level) of the vastus lateralis muscle recorded during a standardized countermovement jump. Higher values indicate greater muscle activation of the vastus lateralis during the jump, whereas lower values indicate reduced activation. Results presented as % from maximal voluntary contraction (MVC) during the procedure of normalization (locked at 90 degrees knee extension)

Outcome measures

Outcome measures
Measure
Treadmill Running
n=22 Participants
Participants jogged on a level treadmill for five minutes at a comfortable pace to provide general muscle warm-up without significant neuromuscular priming. Treadmill running: Participants engaged in a standardized 5-minute treadmill warm-up. Participants were instructed to walk at a moderate, self-selected pace of approximately 7-9 km/h, maintaining an incline of 0%. Heart rate and perceived exertion were monitored to ensure that the activity remained within a light-intensity range (Borg scale: 6-11).
High-Intensity Half-Squat
n=22 Participants
Participants perform one set half-squats using a free-weight barbell in a squat rack to elicit PAPE High-Intensity Half-Squat: Participants in the conditioning arm perform a single set of two to three half-squats with a free-weight barbell set at 90% of their one-rep max (1RM). Each squat is taken down until the thigh is parallel to the floor (knee at \~90°) and then driven upward as explosively as possible.
Muscle Activation During Countermovement Jump in 12 Minute
34.39 percentage of MVC
Standard Deviation 8.23
43.19 percentage of MVC
Standard Deviation 9.49

PRIMARY outcome

Timeframe: Immediate post-intervention

Outcome measures

Outcome measures
Measure
Treadmill Running
n=22 Participants
Participants jogged on a level treadmill for five minutes at a comfortable pace to provide general muscle warm-up without significant neuromuscular priming. Treadmill running: Participants engaged in a standardized 5-minute treadmill warm-up. Participants were instructed to walk at a moderate, self-selected pace of approximately 7-9 km/h, maintaining an incline of 0%. Heart rate and perceived exertion were monitored to ensure that the activity remained within a light-intensity range (Borg scale: 6-11).
High-Intensity Half-Squat
n=22 Participants
Participants perform one set half-squats using a free-weight barbell in a squat rack to elicit PAPE High-Intensity Half-Squat: Participants in the conditioning arm perform a single set of two to three half-squats with a free-weight barbell set at 90% of their one-rep max (1RM). Each squat is taken down until the thigh is parallel to the floor (knee at \~90°) and then driven upward as explosively as possible.
Contraction Time Post
29.04 ms
Standard Deviation 2.45
31.34 ms
Standard Deviation 2.94

PRIMARY outcome

Timeframe: 4 minutes post-intervention

Outcome measures

Outcome measures
Measure
Treadmill Running
n=22 Participants
Participants jogged on a level treadmill for five minutes at a comfortable pace to provide general muscle warm-up without significant neuromuscular priming. Treadmill running: Participants engaged in a standardized 5-minute treadmill warm-up. Participants were instructed to walk at a moderate, self-selected pace of approximately 7-9 km/h, maintaining an incline of 0%. Heart rate and perceived exertion were monitored to ensure that the activity remained within a light-intensity range (Borg scale: 6-11).
High-Intensity Half-Squat
n=22 Participants
Participants perform one set half-squats using a free-weight barbell in a squat rack to elicit PAPE High-Intensity Half-Squat: Participants in the conditioning arm perform a single set of two to three half-squats with a free-weight barbell set at 90% of their one-rep max (1RM). Each squat is taken down until the thigh is parallel to the floor (knee at \~90°) and then driven upward as explosively as possible.
Contraction Time 4 Minute
27.91 ms
Standard Deviation 2.55
29.94 ms
Standard Deviation 2.61

PRIMARY outcome

Timeframe: 6 minutes post-intervention

Outcome measures

Outcome measures
Measure
Treadmill Running
n=22 Participants
Participants jogged on a level treadmill for five minutes at a comfortable pace to provide general muscle warm-up without significant neuromuscular priming. Treadmill running: Participants engaged in a standardized 5-minute treadmill warm-up. Participants were instructed to walk at a moderate, self-selected pace of approximately 7-9 km/h, maintaining an incline of 0%. Heart rate and perceived exertion were monitored to ensure that the activity remained within a light-intensity range (Borg scale: 6-11).
High-Intensity Half-Squat
n=22 Participants
Participants perform one set half-squats using a free-weight barbell in a squat rack to elicit PAPE High-Intensity Half-Squat: Participants in the conditioning arm perform a single set of two to three half-squats with a free-weight barbell set at 90% of their one-rep max (1RM). Each squat is taken down until the thigh is parallel to the floor (knee at \~90°) and then driven upward as explosively as possible.
Contraction Time 6 Minute
27.98 ms
Standard Deviation 2.24
26.87 ms
Standard Deviation 2.32

PRIMARY outcome

Timeframe: 8 minutes post-intervention

Outcome measures

Outcome measures
Measure
Treadmill Running
n=22 Participants
Participants jogged on a level treadmill for five minutes at a comfortable pace to provide general muscle warm-up without significant neuromuscular priming. Treadmill running: Participants engaged in a standardized 5-minute treadmill warm-up. Participants were instructed to walk at a moderate, self-selected pace of approximately 7-9 km/h, maintaining an incline of 0%. Heart rate and perceived exertion were monitored to ensure that the activity remained within a light-intensity range (Borg scale: 6-11).
High-Intensity Half-Squat
n=22 Participants
Participants perform one set half-squats using a free-weight barbell in a squat rack to elicit PAPE High-Intensity Half-Squat: Participants in the conditioning arm perform a single set of two to three half-squats with a free-weight barbell set at 90% of their one-rep max (1RM). Each squat is taken down until the thigh is parallel to the floor (knee at \~90°) and then driven upward as explosively as possible.
Contraction Time 8 Minute
26.43 ms
Standard Deviation 2.05
26.54 ms
Standard Deviation 2.33

PRIMARY outcome

Timeframe: 10 minutes post-intervention

Outcome measures

Outcome measures
Measure
Treadmill Running
n=22 Participants
Participants jogged on a level treadmill for five minutes at a comfortable pace to provide general muscle warm-up without significant neuromuscular priming. Treadmill running: Participants engaged in a standardized 5-minute treadmill warm-up. Participants were instructed to walk at a moderate, self-selected pace of approximately 7-9 km/h, maintaining an incline of 0%. Heart rate and perceived exertion were monitored to ensure that the activity remained within a light-intensity range (Borg scale: 6-11).
High-Intensity Half-Squat
n=22 Participants
Participants perform one set half-squats using a free-weight barbell in a squat rack to elicit PAPE High-Intensity Half-Squat: Participants in the conditioning arm perform a single set of two to three half-squats with a free-weight barbell set at 90% of their one-rep max (1RM). Each squat is taken down until the thigh is parallel to the floor (knee at \~90°) and then driven upward as explosively as possible.
Contraction Time 10 Minute
26.83 ms
Standard Deviation 2.58
26.24 ms
Standard Deviation 2.35

PRIMARY outcome

Timeframe: 12 post-intervention

Outcome measures

Outcome measures
Measure
Treadmill Running
n=22 Participants
Participants jogged on a level treadmill for five minutes at a comfortable pace to provide general muscle warm-up without significant neuromuscular priming. Treadmill running: Participants engaged in a standardized 5-minute treadmill warm-up. Participants were instructed to walk at a moderate, self-selected pace of approximately 7-9 km/h, maintaining an incline of 0%. Heart rate and perceived exertion were monitored to ensure that the activity remained within a light-intensity range (Borg scale: 6-11).
High-Intensity Half-Squat
n=22 Participants
Participants perform one set half-squats using a free-weight barbell in a squat rack to elicit PAPE High-Intensity Half-Squat: Participants in the conditioning arm perform a single set of two to three half-squats with a free-weight barbell set at 90% of their one-rep max (1RM). Each squat is taken down until the thigh is parallel to the floor (knee at \~90°) and then driven upward as explosively as possible.
Contraction Time 12 Minutes
26.29 ms
Standard Deviation 1.77
26.01 ms
Standard Deviation 2.09

PRIMARY outcome

Timeframe: Immediate post-intervention

Outcome measures

Outcome measures
Measure
Treadmill Running
n=22 Participants
Participants jogged on a level treadmill for five minutes at a comfortable pace to provide general muscle warm-up without significant neuromuscular priming. Treadmill running: Participants engaged in a standardized 5-minute treadmill warm-up. Participants were instructed to walk at a moderate, self-selected pace of approximately 7-9 km/h, maintaining an incline of 0%. Heart rate and perceived exertion were monitored to ensure that the activity remained within a light-intensity range (Borg scale: 6-11).
High-Intensity Half-Squat
n=22 Participants
Participants perform one set half-squats using a free-weight barbell in a squat rack to elicit PAPE High-Intensity Half-Squat: Participants in the conditioning arm perform a single set of two to three half-squats with a free-weight barbell set at 90% of their one-rep max (1RM). Each squat is taken down until the thigh is parallel to the floor (knee at \~90°) and then driven upward as explosively as possible.
Delay Time Post
22.54 ms
Standard Deviation 1.33
21.49 ms
Standard Deviation 1.16

PRIMARY outcome

Timeframe: 4 minutes post-intervention

Outcome measures

Outcome measures
Measure
Treadmill Running
n=22 Participants
Participants jogged on a level treadmill for five minutes at a comfortable pace to provide general muscle warm-up without significant neuromuscular priming. Treadmill running: Participants engaged in a standardized 5-minute treadmill warm-up. Participants were instructed to walk at a moderate, self-selected pace of approximately 7-9 km/h, maintaining an incline of 0%. Heart rate and perceived exertion were monitored to ensure that the activity remained within a light-intensity range (Borg scale: 6-11).
High-Intensity Half-Squat
n=22 Participants
Participants perform one set half-squats using a free-weight barbell in a squat rack to elicit PAPE High-Intensity Half-Squat: Participants in the conditioning arm perform a single set of two to three half-squats with a free-weight barbell set at 90% of their one-rep max (1RM). Each squat is taken down until the thigh is parallel to the floor (knee at \~90°) and then driven upward as explosively as possible.
Delay Time 4 Minute
22.41 ms
Standard Deviation 1.35
21.06 ms
Standard Deviation 1.14

PRIMARY outcome

Timeframe: 6 minutes post-intervention

Outcome measures

Outcome measures
Measure
Treadmill Running
n=22 Participants
Participants jogged on a level treadmill for five minutes at a comfortable pace to provide general muscle warm-up without significant neuromuscular priming. Treadmill running: Participants engaged in a standardized 5-minute treadmill warm-up. Participants were instructed to walk at a moderate, self-selected pace of approximately 7-9 km/h, maintaining an incline of 0%. Heart rate and perceived exertion were monitored to ensure that the activity remained within a light-intensity range (Borg scale: 6-11).
High-Intensity Half-Squat
n=22 Participants
Participants perform one set half-squats using a free-weight barbell in a squat rack to elicit PAPE High-Intensity Half-Squat: Participants in the conditioning arm perform a single set of two to three half-squats with a free-weight barbell set at 90% of their one-rep max (1RM). Each squat is taken down until the thigh is parallel to the floor (knee at \~90°) and then driven upward as explosively as possible.
Delay Time 6 Minute
22.52 ms
Standard Deviation 1.38
21.04 ms
Standard Deviation 1.18

PRIMARY outcome

Timeframe: 8 minutes post-intervention

Outcome measures

Outcome measures
Measure
Treadmill Running
n=22 Participants
Participants jogged on a level treadmill for five minutes at a comfortable pace to provide general muscle warm-up without significant neuromuscular priming. Treadmill running: Participants engaged in a standardized 5-minute treadmill warm-up. Participants were instructed to walk at a moderate, self-selected pace of approximately 7-9 km/h, maintaining an incline of 0%. Heart rate and perceived exertion were monitored to ensure that the activity remained within a light-intensity range (Borg scale: 6-11).
High-Intensity Half-Squat
n=22 Participants
Participants perform one set half-squats using a free-weight barbell in a squat rack to elicit PAPE High-Intensity Half-Squat: Participants in the conditioning arm perform a single set of two to three half-squats with a free-weight barbell set at 90% of their one-rep max (1RM). Each squat is taken down until the thigh is parallel to the floor (knee at \~90°) and then driven upward as explosively as possible.
Delay Time 8 Minute
22.07 ms
Standard Deviation 1.36
20.77 ms
Standard Deviation 1.16

PRIMARY outcome

Timeframe: 10 minutes post-intervention

Outcome measures

Outcome measures
Measure
Treadmill Running
n=22 Participants
Participants jogged on a level treadmill for five minutes at a comfortable pace to provide general muscle warm-up without significant neuromuscular priming. Treadmill running: Participants engaged in a standardized 5-minute treadmill warm-up. Participants were instructed to walk at a moderate, self-selected pace of approximately 7-9 km/h, maintaining an incline of 0%. Heart rate and perceived exertion were monitored to ensure that the activity remained within a light-intensity range (Borg scale: 6-11).
High-Intensity Half-Squat
n=22 Participants
Participants perform one set half-squats using a free-weight barbell in a squat rack to elicit PAPE High-Intensity Half-Squat: Participants in the conditioning arm perform a single set of two to three half-squats with a free-weight barbell set at 90% of their one-rep max (1RM). Each squat is taken down until the thigh is parallel to the floor (knee at \~90°) and then driven upward as explosively as possible.
Delay Time 10 Minute
22.84 ms
Standard Deviation 1.86
22.05 ms
Standard Deviation 2.39

PRIMARY outcome

Timeframe: 12 minutes post-intervention

Outcome measures

Outcome measures
Measure
Treadmill Running
n=22 Participants
Participants jogged on a level treadmill for five minutes at a comfortable pace to provide general muscle warm-up without significant neuromuscular priming. Treadmill running: Participants engaged in a standardized 5-minute treadmill warm-up. Participants were instructed to walk at a moderate, self-selected pace of approximately 7-9 km/h, maintaining an incline of 0%. Heart rate and perceived exertion were monitored to ensure that the activity remained within a light-intensity range (Borg scale: 6-11).
High-Intensity Half-Squat
n=22 Participants
Participants perform one set half-squats using a free-weight barbell in a squat rack to elicit PAPE High-Intensity Half-Squat: Participants in the conditioning arm perform a single set of two to three half-squats with a free-weight barbell set at 90% of their one-rep max (1RM). Each squat is taken down until the thigh is parallel to the floor (knee at \~90°) and then driven upward as explosively as possible.
Delay Time 12 Minute
22.88 ms
Standard Deviation 1.91
21.70 ms
Standard Deviation 2.54

PRIMARY outcome

Timeframe: Immediate post-intervention

Outcome measures

Outcome measures
Measure
Treadmill Running
n=22 Participants
Participants jogged on a level treadmill for five minutes at a comfortable pace to provide general muscle warm-up without significant neuromuscular priming. Treadmill running: Participants engaged in a standardized 5-minute treadmill warm-up. Participants were instructed to walk at a moderate, self-selected pace of approximately 7-9 km/h, maintaining an incline of 0%. Heart rate and perceived exertion were monitored to ensure that the activity remained within a light-intensity range (Borg scale: 6-11).
High-Intensity Half-Squat
n=22 Participants
Participants perform one set half-squats using a free-weight barbell in a squat rack to elicit PAPE High-Intensity Half-Squat: Participants in the conditioning arm perform a single set of two to three half-squats with a free-weight barbell set at 90% of their one-rep max (1RM). Each squat is taken down until the thigh is parallel to the floor (knee at \~90°) and then driven upward as explosively as possible.
Relaxation Time Post
16.35 ms
Standard Deviation 5.71
20.97 ms
Standard Deviation 7.46

PRIMARY outcome

Timeframe: 4 minutes post-intervention

Outcome measures

Outcome measures
Measure
Treadmill Running
n=22 Participants
Participants jogged on a level treadmill for five minutes at a comfortable pace to provide general muscle warm-up without significant neuromuscular priming. Treadmill running: Participants engaged in a standardized 5-minute treadmill warm-up. Participants were instructed to walk at a moderate, self-selected pace of approximately 7-9 km/h, maintaining an incline of 0%. Heart rate and perceived exertion were monitored to ensure that the activity remained within a light-intensity range (Borg scale: 6-11).
High-Intensity Half-Squat
n=22 Participants
Participants perform one set half-squats using a free-weight barbell in a squat rack to elicit PAPE High-Intensity Half-Squat: Participants in the conditioning arm perform a single set of two to three half-squats with a free-weight barbell set at 90% of their one-rep max (1RM). Each squat is taken down until the thigh is parallel to the floor (knee at \~90°) and then driven upward as explosively as possible.
Relaxation Time 4 Minute
16.35 ms
Standard Deviation 5.71
19.08 ms
Standard Deviation 7.90

PRIMARY outcome

Timeframe: 6 minutes post-intervention

Outcome measures

Outcome measures
Measure
Treadmill Running
n=22 Participants
Participants jogged on a level treadmill for five minutes at a comfortable pace to provide general muscle warm-up without significant neuromuscular priming. Treadmill running: Participants engaged in a standardized 5-minute treadmill warm-up. Participants were instructed to walk at a moderate, self-selected pace of approximately 7-9 km/h, maintaining an incline of 0%. Heart rate and perceived exertion were monitored to ensure that the activity remained within a light-intensity range (Borg scale: 6-11).
High-Intensity Half-Squat
n=22 Participants
Participants perform one set half-squats using a free-weight barbell in a squat rack to elicit PAPE High-Intensity Half-Squat: Participants in the conditioning arm perform a single set of two to three half-squats with a free-weight barbell set at 90% of their one-rep max (1RM). Each squat is taken down until the thigh is parallel to the floor (knee at \~90°) and then driven upward as explosively as possible.
Relaxation Time 6 Minute
17.57 ms
Standard Deviation 6.20
18.16 ms
Standard Deviation 7.52

PRIMARY outcome

Timeframe: 8 minutes post-intervention

Outcome measures

Outcome measures
Measure
Treadmill Running
n=22 Participants
Participants jogged on a level treadmill for five minutes at a comfortable pace to provide general muscle warm-up without significant neuromuscular priming. Treadmill running: Participants engaged in a standardized 5-minute treadmill warm-up. Participants were instructed to walk at a moderate, self-selected pace of approximately 7-9 km/h, maintaining an incline of 0%. Heart rate and perceived exertion were monitored to ensure that the activity remained within a light-intensity range (Borg scale: 6-11).
High-Intensity Half-Squat
n=22 Participants
Participants perform one set half-squats using a free-weight barbell in a squat rack to elicit PAPE High-Intensity Half-Squat: Participants in the conditioning arm perform a single set of two to three half-squats with a free-weight barbell set at 90% of their one-rep max (1RM). Each squat is taken down until the thigh is parallel to the floor (knee at \~90°) and then driven upward as explosively as possible.
Relaxation Time 8 Minute
19.94 ms
Standard Deviation 8.53
19.28 ms
Standard Deviation 8.02

PRIMARY outcome

Timeframe: 10 minutes post-intervention

Outcome measures

Outcome measures
Measure
Treadmill Running
n=22 Participants
Participants jogged on a level treadmill for five minutes at a comfortable pace to provide general muscle warm-up without significant neuromuscular priming. Treadmill running: Participants engaged in a standardized 5-minute treadmill warm-up. Participants were instructed to walk at a moderate, self-selected pace of approximately 7-9 km/h, maintaining an incline of 0%. Heart rate and perceived exertion were monitored to ensure that the activity remained within a light-intensity range (Borg scale: 6-11).
High-Intensity Half-Squat
n=22 Participants
Participants perform one set half-squats using a free-weight barbell in a squat rack to elicit PAPE High-Intensity Half-Squat: Participants in the conditioning arm perform a single set of two to three half-squats with a free-weight barbell set at 90% of their one-rep max (1RM). Each squat is taken down until the thigh is parallel to the floor (knee at \~90°) and then driven upward as explosively as possible.
Relaxation Time 10 Minute
16.63 ms
Standard Deviation 4.88
17.71 ms
Standard Deviation 7.14

PRIMARY outcome

Timeframe: 12 minutes post-intervention

Outcome measures

Outcome measures
Measure
Treadmill Running
n=22 Participants
Participants jogged on a level treadmill for five minutes at a comfortable pace to provide general muscle warm-up without significant neuromuscular priming. Treadmill running: Participants engaged in a standardized 5-minute treadmill warm-up. Participants were instructed to walk at a moderate, self-selected pace of approximately 7-9 km/h, maintaining an incline of 0%. Heart rate and perceived exertion were monitored to ensure that the activity remained within a light-intensity range (Borg scale: 6-11).
High-Intensity Half-Squat
n=22 Participants
Participants perform one set half-squats using a free-weight barbell in a squat rack to elicit PAPE High-Intensity Half-Squat: Participants in the conditioning arm perform a single set of two to three half-squats with a free-weight barbell set at 90% of their one-rep max (1RM). Each squat is taken down until the thigh is parallel to the floor (knee at \~90°) and then driven upward as explosively as possible.
Relaxation Time 12 Minute
17.30 ms
Standard Deviation 5.48
17.20 ms
Standard Deviation 6.64

PRIMARY outcome

Timeframe: Immediate post-intervention

Outcome measures

Outcome measures
Measure
Treadmill Running
n=22 Participants
Participants jogged on a level treadmill for five minutes at a comfortable pace to provide general muscle warm-up without significant neuromuscular priming. Treadmill running: Participants engaged in a standardized 5-minute treadmill warm-up. Participants were instructed to walk at a moderate, self-selected pace of approximately 7-9 km/h, maintaining an incline of 0%. Heart rate and perceived exertion were monitored to ensure that the activity remained within a light-intensity range (Borg scale: 6-11).
High-Intensity Half-Squat
n=22 Participants
Participants perform one set half-squats using a free-weight barbell in a squat rack to elicit PAPE High-Intensity Half-Squat: Participants in the conditioning arm perform a single set of two to three half-squats with a free-weight barbell set at 90% of their one-rep max (1RM). Each squat is taken down until the thigh is parallel to the floor (knee at \~90°) and then driven upward as explosively as possible.
Muscle Displacement Post
6.37 mm
Standard Deviation 1.35
5.70 mm
Standard Deviation 1.54

PRIMARY outcome

Timeframe: 4 minutes post-intervention

Outcome measures

Outcome measures
Measure
Treadmill Running
n=22 Participants
Participants jogged on a level treadmill for five minutes at a comfortable pace to provide general muscle warm-up without significant neuromuscular priming. Treadmill running: Participants engaged in a standardized 5-minute treadmill warm-up. Participants were instructed to walk at a moderate, self-selected pace of approximately 7-9 km/h, maintaining an incline of 0%. Heart rate and perceived exertion were monitored to ensure that the activity remained within a light-intensity range (Borg scale: 6-11).
High-Intensity Half-Squat
n=22 Participants
Participants perform one set half-squats using a free-weight barbell in a squat rack to elicit PAPE High-Intensity Half-Squat: Participants in the conditioning arm perform a single set of two to three half-squats with a free-weight barbell set at 90% of their one-rep max (1RM). Each squat is taken down until the thigh is parallel to the floor (knee at \~90°) and then driven upward as explosively as possible.
Muscle Displacement 4 Minute
6.36 mm
Standard Deviation 1.46
5.98 mm
Standard Deviation 1.78

PRIMARY outcome

Timeframe: 6 minutes post-intervention

6' post intervention measure

Outcome measures

Outcome measures
Measure
Treadmill Running
n=22 Participants
Participants jogged on a level treadmill for five minutes at a comfortable pace to provide general muscle warm-up without significant neuromuscular priming. Treadmill running: Participants engaged in a standardized 5-minute treadmill warm-up. Participants were instructed to walk at a moderate, self-selected pace of approximately 7-9 km/h, maintaining an incline of 0%. Heart rate and perceived exertion were monitored to ensure that the activity remained within a light-intensity range (Borg scale: 6-11).
High-Intensity Half-Squat
n=22 Participants
Participants perform one set half-squats using a free-weight barbell in a squat rack to elicit PAPE High-Intensity Half-Squat: Participants in the conditioning arm perform a single set of two to three half-squats with a free-weight barbell set at 90% of their one-rep max (1RM). Each squat is taken down until the thigh is parallel to the floor (knee at \~90°) and then driven upward as explosively as possible.
Muscle Displacement 6 Minute
6.83 mm
Standard Deviation 0.90
5.80 mm
Standard Deviation 1.19

PRIMARY outcome

Timeframe: 8 minutes post-intervention

Outcome measures

Outcome measures
Measure
Treadmill Running
n=22 Participants
Participants jogged on a level treadmill for five minutes at a comfortable pace to provide general muscle warm-up without significant neuromuscular priming. Treadmill running: Participants engaged in a standardized 5-minute treadmill warm-up. Participants were instructed to walk at a moderate, self-selected pace of approximately 7-9 km/h, maintaining an incline of 0%. Heart rate and perceived exertion were monitored to ensure that the activity remained within a light-intensity range (Borg scale: 6-11).
High-Intensity Half-Squat
n=22 Participants
Participants perform one set half-squats using a free-weight barbell in a squat rack to elicit PAPE High-Intensity Half-Squat: Participants in the conditioning arm perform a single set of two to three half-squats with a free-weight barbell set at 90% of their one-rep max (1RM). Each squat is taken down until the thigh is parallel to the floor (knee at \~90°) and then driven upward as explosively as possible.
Muscle Displacement 8 Minute
6.59 mm
Standard Deviation 1.04
5.97 mm
Standard Deviation 1.34

PRIMARY outcome

Timeframe: 12 minutes post-intervention

Outcome measures

Outcome measures
Measure
Treadmill Running
n=22 Participants
Participants jogged on a level treadmill for five minutes at a comfortable pace to provide general muscle warm-up without significant neuromuscular priming. Treadmill running: Participants engaged in a standardized 5-minute treadmill warm-up. Participants were instructed to walk at a moderate, self-selected pace of approximately 7-9 km/h, maintaining an incline of 0%. Heart rate and perceived exertion were monitored to ensure that the activity remained within a light-intensity range (Borg scale: 6-11).
High-Intensity Half-Squat
n=22 Participants
Participants perform one set half-squats using a free-weight barbell in a squat rack to elicit PAPE High-Intensity Half-Squat: Participants in the conditioning arm perform a single set of two to three half-squats with a free-weight barbell set at 90% of their one-rep max (1RM). Each squat is taken down until the thigh is parallel to the floor (knee at \~90°) and then driven upward as explosively as possible.
Muscle Displacement 10 Minute
6.22 mm
Standard Deviation 1.11
6.21 mm
Standard Deviation 1.43

PRIMARY outcome

Timeframe: 12 minutes post-intervention

Outcome measures

Outcome measures
Measure
Treadmill Running
n=22 Participants
Participants jogged on a level treadmill for five minutes at a comfortable pace to provide general muscle warm-up without significant neuromuscular priming. Treadmill running: Participants engaged in a standardized 5-minute treadmill warm-up. Participants were instructed to walk at a moderate, self-selected pace of approximately 7-9 km/h, maintaining an incline of 0%. Heart rate and perceived exertion were monitored to ensure that the activity remained within a light-intensity range (Borg scale: 6-11).
High-Intensity Half-Squat
n=22 Participants
Participants perform one set half-squats using a free-weight barbell in a squat rack to elicit PAPE High-Intensity Half-Squat: Participants in the conditioning arm perform a single set of two to three half-squats with a free-weight barbell set at 90% of their one-rep max (1RM). Each squat is taken down until the thigh is parallel to the floor (knee at \~90°) and then driven upward as explosively as possible.
Muscle Displacement 12 Minute
6.23 mm
Standard Deviation 1.21
6.50 mm
Standard Deviation 1.55

PRIMARY outcome

Timeframe: Immediate post-intervention

Outcome measures

Outcome measures
Measure
Treadmill Running
n=22 Participants
Participants jogged on a level treadmill for five minutes at a comfortable pace to provide general muscle warm-up without significant neuromuscular priming. Treadmill running: Participants engaged in a standardized 5-minute treadmill warm-up. Participants were instructed to walk at a moderate, self-selected pace of approximately 7-9 km/h, maintaining an incline of 0%. Heart rate and perceived exertion were monitored to ensure that the activity remained within a light-intensity range (Borg scale: 6-11).
High-Intensity Half-Squat
n=22 Participants
Participants perform one set half-squats using a free-weight barbell in a squat rack to elicit PAPE High-Intensity Half-Squat: Participants in the conditioning arm perform a single set of two to three half-squats with a free-weight barbell set at 90% of their one-rep max (1RM). Each squat is taken down until the thigh is parallel to the floor (knee at \~90°) and then driven upward as explosively as possible.
Sustain Time Post
38.83 ms
Standard Deviation 2.77
48.86 ms
Standard Deviation 3.86

PRIMARY outcome

Timeframe: 4 minutes post-intervention

Outcome measures

Outcome measures
Measure
Treadmill Running
n=22 Participants
Participants jogged on a level treadmill for five minutes at a comfortable pace to provide general muscle warm-up without significant neuromuscular priming. Treadmill running: Participants engaged in a standardized 5-minute treadmill warm-up. Participants were instructed to walk at a moderate, self-selected pace of approximately 7-9 km/h, maintaining an incline of 0%. Heart rate and perceived exertion were monitored to ensure that the activity remained within a light-intensity range (Borg scale: 6-11).
High-Intensity Half-Squat
n=22 Participants
Participants perform one set half-squats using a free-weight barbell in a squat rack to elicit PAPE High-Intensity Half-Squat: Participants in the conditioning arm perform a single set of two to three half-squats with a free-weight barbell set at 90% of their one-rep max (1RM). Each squat is taken down until the thigh is parallel to the floor (knee at \~90°) and then driven upward as explosively as possible.
Sustain Time 4 Minute
39.52 ms
Standard Deviation 2.70
46.23 ms
Standard Deviation 3.74

PRIMARY outcome

Timeframe: 6 minutes post-intervention

Outcome measures

Outcome measures
Measure
Treadmill Running
n=22 Participants
Participants jogged on a level treadmill for five minutes at a comfortable pace to provide general muscle warm-up without significant neuromuscular priming. Treadmill running: Participants engaged in a standardized 5-minute treadmill warm-up. Participants were instructed to walk at a moderate, self-selected pace of approximately 7-9 km/h, maintaining an incline of 0%. Heart rate and perceived exertion were monitored to ensure that the activity remained within a light-intensity range (Borg scale: 6-11).
High-Intensity Half-Squat
n=22 Participants
Participants perform one set half-squats using a free-weight barbell in a squat rack to elicit PAPE High-Intensity Half-Squat: Participants in the conditioning arm perform a single set of two to three half-squats with a free-weight barbell set at 90% of their one-rep max (1RM). Each squat is taken down until the thigh is parallel to the floor (knee at \~90°) and then driven upward as explosively as possible.
Sustain Time 6 Minute
38.82 ms
Standard Deviation 4.52
43.27 ms
Standard Deviation 6.52

PRIMARY outcome

Timeframe: 8 minutes post-intervention

Outcome measures

Outcome measures
Measure
Treadmill Running
n=22 Participants
Participants jogged on a level treadmill for five minutes at a comfortable pace to provide general muscle warm-up without significant neuromuscular priming. Treadmill running: Participants engaged in a standardized 5-minute treadmill warm-up. Participants were instructed to walk at a moderate, self-selected pace of approximately 7-9 km/h, maintaining an incline of 0%. Heart rate and perceived exertion were monitored to ensure that the activity remained within a light-intensity range (Borg scale: 6-11).
High-Intensity Half-Squat
n=22 Participants
Participants perform one set half-squats using a free-weight barbell in a squat rack to elicit PAPE High-Intensity Half-Squat: Participants in the conditioning arm perform a single set of two to three half-squats with a free-weight barbell set at 90% of their one-rep max (1RM). Each squat is taken down until the thigh is parallel to the floor (knee at \~90°) and then driven upward as explosively as possible.
Sustain Time 8 Minute
39.03 ms
Standard Deviation 3.50
42.36 ms
Standard Deviation 4.33

PRIMARY outcome

Timeframe: 10 minutes post-intervention

Outcome measures

Outcome measures
Measure
Treadmill Running
n=22 Participants
Participants jogged on a level treadmill for five minutes at a comfortable pace to provide general muscle warm-up without significant neuromuscular priming. Treadmill running: Participants engaged in a standardized 5-minute treadmill warm-up. Participants were instructed to walk at a moderate, self-selected pace of approximately 7-9 km/h, maintaining an incline of 0%. Heart rate and perceived exertion were monitored to ensure that the activity remained within a light-intensity range (Borg scale: 6-11).
High-Intensity Half-Squat
n=22 Participants
Participants perform one set half-squats using a free-weight barbell in a squat rack to elicit PAPE High-Intensity Half-Squat: Participants in the conditioning arm perform a single set of two to three half-squats with a free-weight barbell set at 90% of their one-rep max (1RM). Each squat is taken down until the thigh is parallel to the floor (knee at \~90°) and then driven upward as explosively as possible.
Sustain Time 10 Minutes
39.11 ms
Standard Deviation 4.31
42.91 ms
Standard Deviation 5.47

PRIMARY outcome

Timeframe: 12 minutes post-intervention

Outcome measures

Outcome measures
Measure
Treadmill Running
n=22 Participants
Participants jogged on a level treadmill for five minutes at a comfortable pace to provide general muscle warm-up without significant neuromuscular priming. Treadmill running: Participants engaged in a standardized 5-minute treadmill warm-up. Participants were instructed to walk at a moderate, self-selected pace of approximately 7-9 km/h, maintaining an incline of 0%. Heart rate and perceived exertion were monitored to ensure that the activity remained within a light-intensity range (Borg scale: 6-11).
High-Intensity Half-Squat
n=22 Participants
Participants perform one set half-squats using a free-weight barbell in a squat rack to elicit PAPE High-Intensity Half-Squat: Participants in the conditioning arm perform a single set of two to three half-squats with a free-weight barbell set at 90% of their one-rep max (1RM). Each squat is taken down until the thigh is parallel to the floor (knee at \~90°) and then driven upward as explosively as possible.
Sustain Time 12 Minute
40.77 ms
Standard Deviation 3.72
43.47 ms
Standard Deviation 4.97

Adverse Events

High-Intensity Half-Squat

Serious events: 0 serious events
Other events: 0 other events
Deaths: 0 deaths

Treadmill Running

Serious events: 0 serious events
Other events: 0 other events
Deaths: 0 deaths

Serious adverse events

Adverse event data not reported

Other adverse events

Adverse event data not reported

Additional Information

Katarzyna Sadowska

Jerzy Kukuczka Academy of Physical Education

Phone: 32 207 5189

Results disclosure agreements

  • Principal investigator is a sponsor employee
  • Publication restrictions are in place