Trial Outcomes & Findings for Histopathological Evaluation of the Periodontal Ligament Subjected to Laser Ablation. (NCT NCT06173648)

Single study group that requires extraction of mandibular incisors for other reasons. Teeth will be accessed, subject to mechanical file, pulp extirpation, and treated with endodontic solutions. Teeth will then be exposed to the ICG solution followed by laser exposure of the ICG solution in situ. After laser exposure, the pulp chamber and roots will be rinsed and then the tooth mechanically extracted. After extraction, tissue specimens will be prepared of the root portion of the extracted tooth, fixed, stained, and mounted for visual examination under a microscope. Images of the microscopic images will be captured for documentation. The samples will be examined for the histological and morphological condition of the periodontal ligament, cementum, and collagen attached to the external surface of the extracted tooth root. These tissue types were graded based on the extent of damage or destruction observed. Laser and ICG solution.: Intervention consists of addition of laser absorption solution and infrared diode laser. The solution is introduced into pulp chamber and root canals, dwells for 1 minute, followed by exposure to the laser delivered via optical fiber introduced into the root canal, activating the laser at 2.5W optical output, pulse duration of 100msec and pulse gap of 300msec, with laser emissions totaling 30s, moving and relocating the fiber during the exposure. The laser is halted, a 30s wait, then solution and laser is repeated.

n=12 Teeth

Single study group that requires extraction of mandibular incisors for other reasons. Teeth will be accessed, subject to mechanical file, pulp extirpation, and treated with endodontic solutions. Teeth will then be exposed to the ICG solution followed by laser exposure of the ICG solution in situ. After laser exposure, the pulp chamber and roots will be rinsed and then the tooth mechanically extracted. After extraction, tissue specimens will be prepared of the root portion of the extracted tooth, fixed, stained, and mounted for visual examination under a microscope. Images of the microscopic images will be captured for documentation. The samples will be examined for the histological and morphological condition of the periodontal ligament, cementum, and collagen attached to the external surface of the extracted tooth root. These tissue types were graded based on the extent of damage or destruction observed. Laser and ICG solution.: Intervention consists of addition of laser absorption solution and infrared diode laser. The solution is introduced into pulp chamber and root canals, dwells for 1 minute, followed by exposure to the laser delivered via optical fiber introduced into the root canal, activating the laser at 2.5W optical output, pulse duration of 100msec and pulse gap of 300msec, with laser emissions totaling 30s, moving and relocating the fiber during the exposure. The laser is halted, a 30s wait, then solution and laser is repeated.

n=12 Teeth

Single study group that requires extraction of mandibular incisors for other reasons. Teeth will be accessed, subject to mechanical file, pulp extirpation, and treated with endodontic solutions. Teeth will then be exposed to the ICG solution followed by laser exposure of the ICG solution in situ. After laser exposure, the pulp chamber and roots will be rinsed and then the tooth mechanically extracted. After extraction, tissue specimens will be prepared of the root portion of the extracted tooth, fixed, stained, and mounted for visual examination under a microscope. Images of the microscopic images will be captured for documentation. The samples will be examined for the histological and morphological condition of the periodontal ligament, cementum, and collagen attached to the external surface of the extracted tooth root. These tissue types were graded based on the extent of damage or destruction observed. Laser and ICG solution.: Intervention consists of addition of laser absorption solution and infrared diode laser. The solution is introduced into pulp chamber and root canals, dwells for 1 minute, followed by exposure to the laser delivered via optical fiber introduced into the root canal, activating the laser at 2.5W optical output, pulse duration of 100msec and pulse gap of 300msec, with laser emissions totaling 30s, moving and relocating the fiber during the exposure. The laser is halted, a 30s wait, then solution and laser is repeated.

n=12 Teeth

Single study group that requires extraction of mandibular incisors for other reasons. Teeth will be accessed, subject to mechanical file, pulp extirpation, and treated with endodontic solutions. Teeth will then be exposed to the ICG solution followed by laser exposure of the ICG solution in situ. After laser exposure, the pulp chamber and roots will be rinsed and then the tooth mechanically extracted. After extraction, tissue specimens will be prepared of the root portion of the extracted tooth, fixed, stained, and mounted for visual examination under a microscope. Images of the microscopic images will be captured for documentation. The samples will be examined for the histological and morphological condition of the periodontal ligament, cementum, and collagen attached to the external surface of the extracted tooth root. These tissue types were graded based on the extent of damage or destruction observed. Laser and ICG solution.: Intervention consists of addition of laser absorption solution and infrared diode laser. The solution is introduced into pulp chamber and root canals, dwells for 1 minute, followed by exposure to the laser delivered via optical fiber introduced into the root canal, activating the laser at 2.5W optical output, pulse duration of 100msec and pulse gap of 300msec, with laser emissions totaling 30s, moving and relocating the fiber during the exposure. The laser is halted, a 30s wait, then solution and laser is repeated.

n=12 Teeth

Single study group that requires extraction of mandibular incisors for other reasons. Teeth will be accessed, subject to mechanical file, pulp extirpation, and treated with endodontic solutions. Teeth will then be exposed to the ICG solution followed by laser exposure of the ICG solution in situ. After laser exposure, the pulp chamber and roots will be rinsed and then the tooth mechanically extracted. After extraction, tissue specimens will be prepared of the root portion of the extracted tooth, fixed, stained, and mounted for visual examination under a microscope. Images of the microscopic images will be captured for documentation. The samples will be examined for the histological and morphological condition of the periodontal ligament, cementum, and collagen attached to the external surface of the extracted tooth root. These tissue types were graded based on the extent of damage or destruction observed. Laser and ICG solution.: Intervention consists of addition of laser absorption solution and infrared diode laser. The solution is introduced into pulp chamber and root canals, dwells for 1 minute, followed by exposure to the laser delivered via optical fiber introduced into the root canal, activating the laser at 2.5W optical output, pulse duration of 100msec and pulse gap of 300msec, with laser emissions totaling 30s, moving and relocating the fiber during the exposure. The laser is halted, a 30s wait, then solution and laser is repeated.

n=12 Teeth

Single study group that requires extraction of mandibular incisors for other reasons. Teeth will be accessed, subject to mechanical file, pulp extirpation, and treated with endodontic solutions. Teeth will then be exposed to the ICG solution followed by laser exposure of the ICG solution in situ. After laser exposure, the pulp chamber and roots will be rinsed and then the tooth mechanically extracted. After extraction, tissue specimens will be prepared of the root portion of the extracted tooth, fixed, stained, and mounted for visual examination under a microscope. Images of the microscopic images will be captured for documentation. The samples will be examined for the histological and morphological condition of the periodontal ligament, cementum, and collagen attached to the external surface of the extracted tooth root. These tissue types were graded based on the extent of damage or destruction observed. Laser and ICG solution.: Intervention consists of addition of laser absorption solution and infrared diode laser. The solution is introduced into pulp chamber and root canals, dwells for 1 minute, followed by exposure to the laser delivered via optical fiber introduced into the root canal, activating the laser at 2.5W optical output, pulse duration of 100msec and pulse gap of 300msec, with laser emissions totaling 30s, moving and relocating the fiber during the exposure. The laser is halted, a 30s wait, then solution and laser is repeated.