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Spatial Analysis of Host-parasite Interactions in Cutaneous Leishmaniasis in Ethiopia

NCT05332093 · Status: COMPLETED · Type: OBSERVATIONAL · Enrollment: 92

Last updated 2025-01-09

No results posted yet for this study

Summary

Cutaneous leishmaniasis manifestations range from self-healing localized skin ulcers/nodules to diffusely spread chronic lesions. Knowledge on the host-parasite interactions underpinning the different clinical presentations is scarce, in particular for L. aethiopica infections where disease can be extremely severe. Our aim is to define differences in skin immune responses and parasite virulence in CL patients at single cell/parasite level and how it underpins the different clinical presentations (localised, mucocutaneous and diffuse), by producing the first spatially-resolved 'ecological' map of the lesions.

Conditions

  • Cutaneous Leishmaniases

Interventions

DIAGNOSTIC_TEST

skin biopsy

4mm skin biopsy

DIAGNOSTIC_TEST

venous blood sample (plasma, PBMC, WB)

venous blood sample to acquire plasma, PBMCs and whole blood

GENETIC

venous blood sample (HLA)

venous blood sample used for HLA typing

GENETIC

skin slit

genome sequencing of parasite DNA that is extracted from the skin slit

Sponsors & Collaborators

  • University of Gondar

    collaborator OTHER

  • University of York

    collaborator OTHER

  • Maastricht University

    collaborator OTHER

  • University Hospital, Antwerp

    collaborator OTHER

  • Institute of Tropical Medicine, Belgium

    lead OTHER

Principal Investigators

  • Wim Adriaensen, PhD · Institute of Tropical Medicine Antwerp

  • Mikias Woldetensay, MD · University of Gondar

Eligibility

Min Age
12 Years
Max Age
50 Years
Sex
ALL
Healthy Volunteers
No

Timeline & Regulatory

Start
2022-03-21
Primary Completion
2024-03-30
Completion
2024-12-31

Countries

  • Ethiopia

Study Locations

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Read the full study record

This page highlights key information. For complete eligibility criteria, study locations, investigator contacts, and the full protocol, visit the original record on ClinicalTrials.gov.

View NCT05332093 on ClinicalTrials.gov