Trial Outcomes & Findings for The INVIGORATE 2 Trial: A Clinical Trial to Assess the Efficacy and Safety of Subjects With Seasonal Allergic Conjunctivitis (NCT NCT05234554)

NCT ID: NCT05234554

Last Updated: 2026-05-14

Results Overview

The method of assessment was a 9-point ocular itch scale measured in half-unit increments (0 none - 4 severe) with a higher score representing worse symptomology. Subjects were dosed once just prior to allergen chamber entry and again halfway through the chamber. The allergen chamber was 210 ten minutes in duration with the itching assessment being collected every 10 minutes from 110 to 210 minutes. The timepoints were assessed using mixed model repeated measures.

Recruitment status

COMPLETED

Study phase

PHASE3

Target enrollment

131 participants

Primary outcome timeframe

110 to 210 minutes during allergen chamber exposure

Results posted on

2026-05-14

Participant Flow

Participant milestones

Participant milestones
Measure
Reproxalap, Then Vehicle
Subjects first received reproxalap ophthalmic solution (0.25%) twice in a single day. After a washout period of approximately two weeks, subjects then received vehicle ophthalmic solution twice in a single day.
Vehicle, Then Reproxalap
Subjects first received vehicle ophthalmic solution twice in a single day. After a washout period of approximately two weeks, subjects then received reproxalap ophthalmic solution (0.25%) twice in a single day.
Overall Study
STARTED
66
65
Overall Study
COMPLETED
66
64
Overall Study
NOT COMPLETED
0
1

Reasons for withdrawal

Withdrawal data not reported

Baseline Characteristics

The INVIGORATE 2 Trial: A Clinical Trial to Assess the Efficacy and Safety of Subjects With Seasonal Allergic Conjunctivitis

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
Reproxalap, Then Vehicle
n=66 Participants
Subjects first received reproxalap ophthalmic solution (0.25%) twice in a single day. After a washout period of approximately two weeks, subjects then received vehicle ophthalmic solution twice in a single day.
Vehicle, Then Reproxalap
n=65 Participants
Subjects first received vehicle ophthalmic solution twice in a single day. After a washout period of approximately two weeks, subjects then received reproxalap ophthalmic solution (0.25%) twice in a single day.
Total
n=131 Participants
Total of all reporting groups
Age, Continuous
40.9 years
STANDARD_DEVIATION 11.0 • n=1512 Participants
41.9 years
STANDARD_DEVIATION 12.5 • n=504 Participants
41.4 years
STANDARD_DEVIATION 11.7 • n=2016 Participants
Sex: Female, Male
Female
38 Participants
n=1512 Participants
34 Participants
n=504 Participants
72 Participants
n=2016 Participants
Sex: Female, Male
Male
28 Participants
n=1512 Participants
31 Participants
n=504 Participants
59 Participants
n=2016 Participants
Ethnicity (NIH/OMB)
Hispanic or Latino
7 Participants
n=1512 Participants
10 Participants
n=504 Participants
17 Participants
n=2016 Participants
Ethnicity (NIH/OMB)
Not Hispanic or Latino
59 Participants
n=1512 Participants
55 Participants
n=504 Participants
114 Participants
n=2016 Participants
Ethnicity (NIH/OMB)
Unknown or Not Reported
0 Participants
n=1512 Participants
0 Participants
n=504 Participants
0 Participants
n=2016 Participants
Race (NIH/OMB)
American Indian or Alaska Native
2 Participants
n=1512 Participants
1 Participants
n=504 Participants
3 Participants
n=2016 Participants
Race (NIH/OMB)
Asian
11 Participants
n=1512 Participants
10 Participants
n=504 Participants
21 Participants
n=2016 Participants
Race (NIH/OMB)
Native Hawaiian or Other Pacific Islander
1 Participants
n=1512 Participants
1 Participants
n=504 Participants
2 Participants
n=2016 Participants
Race (NIH/OMB)
Black or African American
11 Participants
n=1512 Participants
17 Participants
n=504 Participants
28 Participants
n=2016 Participants
Race (NIH/OMB)
White
37 Participants
n=1512 Participants
34 Participants
n=504 Participants
71 Participants
n=2016 Participants
Race (NIH/OMB)
More than one race
4 Participants
n=1512 Participants
2 Participants
n=504 Participants
6 Participants
n=2016 Participants
Race (NIH/OMB)
Unknown or Not Reported
0 Participants
n=1512 Participants
0 Participants
n=504 Participants
0 Participants
n=2016 Participants
Region of Enrollment
Canada
66 participants
n=1512 Participants
65 participants
n=504 Participants
131 participants
n=2016 Participants

PRIMARY outcome

Timeframe: 110 to 210 minutes during allergen chamber exposure

Population: Intent-to-treat population

The method of assessment was a 9-point ocular itch scale measured in half-unit increments (0 none - 4 severe) with a higher score representing worse symptomology. Subjects were dosed once just prior to allergen chamber entry and again halfway through the chamber. The allergen chamber was 210 ten minutes in duration with the itching assessment being collected every 10 minutes from 110 to 210 minutes. The timepoints were assessed using mixed model repeated measures.

Outcome measures

Outcome measures
Measure
Reproxalap
n=131 Participants
Reproxalap ophthalmic solution (0.25%) was dosed twice in a single day.
Vehicle
n=131 Participants
Vehicle ophthalmic solution was dosed twice in a single day.
Subject-Reported Ocular Itching Score Assessed in the Allergy Chamber
1.48 units on a scale
Standard Error 0.07
1.84 units on a scale
Standard Error 0.07

SECONDARY outcome

Timeframe: 12 to 212 minutes during allergen chamber exposure

Population: Intent-to-treat population

The method of assessment was a 9-point ocular redness scale measured in half-unit increments (0 none - 4 extremely severe) with a higher score representing worse symptomology. Subjects were dosed once just prior to allergen chamber entry and again halfway through the chamber. The allergen chamber was 212 minutes in duration with the redness assessment being collected every 10 minutes from 12 to 212 minutes. The timepoints were assessed using mixed model repeated measures.

Outcome measures

Outcome measures
Measure
Reproxalap
n=131 Participants
Reproxalap ophthalmic solution (0.25%) was dosed twice in a single day.
Vehicle
n=131 Participants
Vehicle ophthalmic solution was dosed twice in a single day.
Conjunctival Redness Evaluated by the Investigator
0.69 units on a scale
Standard Error 0.02
0.71 units on a scale
Standard Error 0.02

Adverse Events

Reproxalap

Serious events: 0 serious events
Other events: 93 other events
Deaths: 0 deaths

Vehicle

Serious events: 0 serious events
Other events: 5 other events
Deaths: 0 deaths

Serious adverse events

Adverse event data not reported

Other adverse events

Other adverse events
Measure
Reproxalap
n=130 participants at risk
Reproxalap ophthalmic solution (0.25%) was dosed twice in a single day.
Vehicle
n=131 participants at risk
Vehicle ophthalmic solution was dosed twice in a single day.
General disorders
General disorders and administration site conditions
71.5%
93/130 • Number of events 93 • The safety assessment period was approximately 4 hours on the study day for each intervention.
3.8%
5/131 • Number of events 5 • The safety assessment period was approximately 4 hours on the study day for each intervention.

Additional Information

Director of Clinical Trials

Aldeyra Therapeutics, Inc.

Phone: 781-257-3063

Results disclosure agreements

  • Principal investigator is a sponsor employee
  • Publication restrictions are in place