MedTrace Logo

Salivary Immune/Stress Biomarkers Among Children

NCT05021380 · Status: UNKNOWN · Type: OBSERVATIONAL · Enrollment: 50

Last updated 2021-08-25

No results posted yet for this study

Summary

Background: The use of easily accessible biomarkers for assessing young patients' health is important. This study's aim is a measuring stress/immune biomarkers in saliva of healthy school-age children and compare subgroups according to age, sex, stress perception in dental pain related to symptomatic irreversible pulpitis (SIP) and symptomatic apical periodontitis (SAP).

Material and methods: 50 children diagnosed with SIP and SAP aged from 6 to 12 years old will be treated with root canal treatment. Dental examination using DMF score and oral hygiene level will be performedby experienced dentists. Salivary samples will be collected three times: before treatment in day of first dental visit (1), after two weeks (2), and after next two weeks = 30 days (3). Additionally, pain and stress perception will be examined by VAS scale and questionnaires dedicated for children anxiety: Frankl behavior rating scale, Venham's anxiety and behavior rating scale. Salivary immunoglobullins A, G, M, opiorphin, free cortisol and amylase will be measured using commercially available ELISA kits. Results will assess which of the measured salivary biomarkers is related to stress and dental pain, suggesting its use for evaluating in non-invasive way in childhood.

Conditions

  • Stress, Emotional
  • Dental Anxiety
  • Dental Pain and Sensation Disorder
  • Cortisol; Hypersecretion

Interventions

OTHER

GROUP

Non-invasive diagnosis of dental pain, dental root canal treatment, salivary specimen collection from the first visit to next and the last during 4 weeks of dental care

Sponsors & Collaborators

  • University Paris 7 - Denis Diderot

    collaborator OTHER

  • Poznan University of Medical Sciences

    lead OTHER

Principal Investigators

  • Elzbieta Paszynska, Prof · Poznan University of Medical Sciences, Poland

  • Yves Boucher, Prof · Université de Paris & Groupe Hospitalier Pitié Salpêtrière, 75006 Paris, France

Eligibility

Min Age
6 Years
Max Age
12 Years
Sex
ALL
Healthy Volunteers
Yes

Timeline & Regulatory

Start
2021-08-01
Primary Completion
2022-03-30
Completion
2022-06-30

Countries

  • Poland

Study Locations

More Related Trials

Read the full study record

This page highlights key information. For complete eligibility criteria, study locations, investigator contacts, and the full protocol, visit the original record on ClinicalTrials.gov.

View NCT05021380 on ClinicalTrials.gov