Clinical, Functional, Immunological and Genetic Factors on the Severity of the Course of Coronavirus Infection

Summary

The purpose of the program. To determine the clinical, functional, immunological, and genetic factors affecting the severity of the course of acute coronavirus infection COVID-19 and PostCovid syndrome, in order to develop management tactics for such patients to reduce the risk of complications and disability.

Conditions

• Covid19
• Corona Virus Infection

Interventions

DIAGNOSTIC_TEST

Clinical and functional analysis

General blood analysis - Complete blood count on an analyzer with differentiation of 5 classes of cells, the ratio of neutrophils to lymphocytes; Blood chemistry - ALT, AST, total bilirubin, direct, LDH, CRP, alpha-amylase, creatinine, urea, glucose, ferritin, glycosylated hemoglobin, vitamin 25 - OH vitamin D, vitamin B12; Coagulogram - D-dimers, fibrinogen, INR, APTT; Other - NT-pro BNP, Homocysteine, IL 6, Troponin, blood group determination; Linked immunosorbent assay - Determination of IgG and IgM antibodies to SARS-CoV-2 coronavirus (COVID-19) in blood serum, RBD; Functional diagnostics • ECG * EchoCG + strain * CT scan of the lungs * Holter * SMAD * Kidney ultrasound * Ultrasound * Doppler ultrasonography of veins and arteries * Chalder Scale, EQ Questionnaire

DIAGNOSTIC_TEST

Neurological analysis

Neurological examination with the isolation of neurological syndromes (motor, cognitive impairments, sleep disorders, asthenic-depressive syndromes, etc.). 2\. Neuropsychological methods - research on the scales of anxiety and depression, MMSE, etc. 3\. Instrumental method - EEG, polysonography, ultrasound of the neck vessels, CT perfusion. 4\. Laboratory research methods: 1. The study of antibodies to some neurospecific antigens - myelin basic protein (MBP), neurospecific enolase (NSE). 2. Study of cellular immunity (CD3 +, CD4 +, CD8 +) and general indicators of humoral immunity (IgG, IgA, IgM, circulating immune complexes).

DIAGNOSTIC_TEST

Immunological analysis:

A comprehensive immunological analysis will be carried out to determine the level of the immune response. Calculation of the level of CD4 +, CD8 + and NK cells. The level of antibodies of the IgG and IgM classes to the proteins of the coronavirus S1, RBD and N was determined

DIAGNOSTIC_TEST

Genetic analysis:

Isolation (extraction) of DNA will be performed from whole blood using commercial kits according to the manufacturer's instructions. To analyze a large number of genetic markers, it is planned to carry out genome-wide sequencing followed by analysis of genetic polymorphisms of candidate genes encoding coronavirus receptors and immunological factors.

Sponsors & Collaborators

• Nazarbayev University Medical Center

  collaborator OTHER

• National Research Center for Cardiac Surgery, Kazakhstan

  lead OTHER

Principal Investigators

• Makhabbat Sansyzbaeva · Chairman of the Board of "National Research Cardiac Surgery Centre" JSC

Eligibility

Min Age

18 Years

Max Age

65 Years

Sex

ALL

Healthy Volunteers

No

Timeline & Regulatory

Start

2021-06-01

Primary Completion

2022-12-01

Completion

2023-06-01

Countries

• Kazakhstan

Study Locations