Trial Outcomes & Findings for LetSync: Pilot Test of Mobile Health (mHealth) Intervention (NCT NCT04951544)

NCT ID: NCT04951544

Last Updated: 2026-03-23

Results Overview

The investigators will use self-collected urine samples as another objective measure of drug adherence for participants living with HIV. The investigators will mail participants a urine sample collection kit with necessary supplies and detailed instructions via online demonstration video. Participants will self-collect and administer the urine sample and send a photo of the results to study staff. Urine test results were collected between baseline (T0) and 8 months (T4) as proportion of samples returned.

Recruitment status

COMPLETED

Study phase

NA

Target enrollment

144 participants

Primary outcome timeframe

8 months

Results posted on

2026-03-23

Participant Flow

Participants were recruited via social media (e.g., dating apps, Facebook, Twitter/X, Instagram), UCSF patient mailing, engagement of community-based organizations, study material distribution via email listservs, and word-of-mouth.

The sampling and analysis approaches differ: To be eligible, Index participants needed to enroll with their partner (i.e., Partner participants). For analyses, we do not separate by Index vs. Partner individuals because we do not analyze individuals as "Index" vs. "Partner", i.e., we include all individuals who provide the outcomes data of interest. Index participants could continue in the study if their partner discontinued.

Participant milestones

Participant milestones
Measure
Intervention Arm
This is the Intervention Arm of the LetSync Pilot Study to evaluate the feasibility and acceptability of the LetSync app intervention and associated protocols and procedures to inform a full-scale RCT. The Intervention Arm received the LetSync app to use through the 8-month trial.
Control Arm
This is the Control Arm of the LetSync Pilot Study to evaluate the feasibility and acceptability of the LetSync app intervention and associated protocols and procedures to inform a full-scale RCT. The Control Arm did not receive the LetSync app.
Overall Study
STARTED
70
74
Overall Study
COMPLETED
62
72
Overall Study
NOT COMPLETED
8
2

Reasons for withdrawal

Reasons for withdrawal
Measure
Intervention Arm
This is the Intervention Arm of the LetSync Pilot Study to evaluate the feasibility and acceptability of the LetSync app intervention and associated protocols and procedures to inform a full-scale RCT. The Intervention Arm received the LetSync app to use through the 8-month trial.
Control Arm
This is the Control Arm of the LetSync Pilot Study to evaluate the feasibility and acceptability of the LetSync app intervention and associated protocols and procedures to inform a full-scale RCT. The Control Arm did not receive the LetSync app.
Overall Study
Participant requested that their data be deleted from the study.
1
0
Overall Study
Lost to Follow-up
7
2

Baseline Characteristics

LetSync: Pilot Test of Mobile Health (mHealth) Intervention

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
Total
n=143 Participants
Total of all reporting groups
Intervention Arm
n=69 Participants
This is the Intervention Arm of the LetSync Pilot Study to evaluate the feasibility and acceptability of the LetSync app intervention and associated protocols and procedures to inform a full-scale RCT. The Intervention Arm received the LetSync app to use through the 8-month trial. The unit of randomization was the dyad, i.e., each dyad - not an individual - was randomized to either the intervention or control. We did not randomize by individual index or partner participant; we randomized by the dyad, i.e., the couple together. Each arm contained both members of each dyad.
Control Arm
n=74 Participants
This is the Control Arm of the LetSync Pilot Study to evaluate the feasibility and acceptability of the LetSync app intervention and associated protocols and procedures to inform a full-scale RCT. The Control Arm did not receive the LetSync app. The unit of randomization was the dyad, i.e., each dyad - not an individual - was randomized to either the intervention or control. We did not randomize by individual index or partner participant; we randomized by the dyad, i.e., the couple together. Each arm contained both members of each dyad.
Age, Categorical
<=18 years
0 Participants
n=18 Participants
0 Participants
n=10 Participants
0 Participants
n=8 Participants
Age, Categorical
Between 18 and 65 years
133 Participants
n=18 Participants
64 Participants
n=10 Participants
69 Participants
n=8 Participants
Age, Categorical
>=65 years
10 Participants
n=18 Participants
5 Participants
n=10 Participants
5 Participants
n=8 Participants
Sex/Gender, Customized
Male or man
128 Participants
n=18 Participants
64 Participants
n=10 Participants
64 Participants
n=8 Participants
Sex/Gender, Customized
Gender-minority
15 Participants
n=18 Participants
5 Participants
n=10 Participants
10 Participants
n=8 Participants
Race/Ethnicity, Customized
Non-white
120 Participants
n=18 Participants
60 Participants
n=10 Participants
60 Participants
n=8 Participants
Race/Ethnicity, Customized
White
23 Participants
n=18 Participants
9 Participants
n=10 Participants
14 Participants
n=8 Participants

PRIMARY outcome

Timeframe: 8 months

Population: The sampling and analysis approaches differ: To be eligible, Index participants needed to enroll with their partner (i.e., Partner participants). However, for data analyses of the outcomes, we do not separate by Index vs. Partner individuals because we do not analyze individuals as "Index" vs. "Partner", i.e., we include all individuals who provide the outcomes data of interest. In this case, it is the Proportion of urine samples returned by anyone enrolled in the study.

The investigators will use self-collected urine samples as another objective measure of drug adherence for participants living with HIV. The investigators will mail participants a urine sample collection kit with necessary supplies and detailed instructions via online demonstration video. Participants will self-collect and administer the urine sample and send a photo of the results to study staff. Urine test results were collected between baseline (T0) and 8 months (T4) as proportion of samples returned.

Outcome measures

Outcome measures
Measure
Intervention Arm
n=69 Participants
This is the Intervention Arm of the LetSync Pilot Study to evaluate the feasibility and acceptability of the LetSync app intervention and associated protocols and procedures to inform a full-scale RCT. The Intervention Arm received the LetSync app to use through the 8-month trial.
Control Arm
n=74 Participants
This is the Control Arm of the LetSync Pilot Study to evaluate the feasibility and acceptability of the LetSync app intervention and associated protocols and procedures to inform a full-scale RCT. The Control Arm did not receive the LetSync app.
Proportion of Urine Samples Returned by Participants
54 Participants
65 Participants

PRIMARY outcome

Timeframe: 8 months

Population: The sampling and analysis approaches differ: To be eligible, Index participants needed to enroll with their partner (i.e., Partner participants). However, for data analyses of the outcomes, we do not separate by Index vs. Partner individuals because we do not analyze individuals as "Index" vs. "Partner", i.e., we include all individuals who provide the outcomes data of interest. In this case, it is the Levels of Antiretroviral (ARV, TFVDP) concentration in dried blood spot (DBS) samples.

The investigators will use the UNC Pharmacology Lab to analyze ARV levels in dried blood spot (DBS) samples as an objective measure of drug adherence and exposure for HIV-infected participants. The investigators will mail participants a DBS sample collection kit with necessary supplies, detailed instructions via online demonstration video, and a prepaid return envelope. DBS samples will be collected and analyzed between baseline (T0) and 8 months (T4). ARV levels of TFVDP will be measured as nanograms per milligram (ng/mg) of DBS will be log-transformed for data analysis. Changes since Baseline of ARV levels will be measured.

Outcome measures

Outcome measures
Measure
Intervention Arm
n=54 Participants
This is the Intervention Arm of the LetSync Pilot Study to evaluate the feasibility and acceptability of the LetSync app intervention and associated protocols and procedures to inform a full-scale RCT. The Intervention Arm received the LetSync app to use through the 8-month trial.
Control Arm
n=59 Participants
This is the Control Arm of the LetSync Pilot Study to evaluate the feasibility and acceptability of the LetSync app intervention and associated protocols and procedures to inform a full-scale RCT. The Control Arm did not receive the LetSync app.
Levels of Antiretroviral (ARV, TFVDP) Concentration in Dried Blood Spot (DBS) Samples
485.03 ng/mg
Standard Deviation 603.42
595.55 ng/mg
Standard Deviation 1172.40

PRIMARY outcome

Timeframe: 8 months

Population: The sampling and analysis approaches differ: To be eligible, Index participants needed to enroll with their partner (i.e., Partner participants). However, for data analyses of the outcomes, we do not separate by Index vs. Partner individuals because we do not analyze individuals as "Index" vs. "Partner", i.e., we include all individuals who provide the outcomes data of interest. In this case, it is the Self-reported measure of care engagement.

Self-reported care engagement will be measured with the 10-item Index of Engagement in HIV Care rated on a Likert-type scale ranging from 1 to 5, 'Strongly disagree' to 'Strongly agree'. The minimum summed score is 10, the maximum summed score is 50. A score will be calculated is the sum of all items without missing responses, with a higher score representing better care engagement and lower score representing worse care engagement.

Outcome measures

Outcome measures
Measure
Intervention Arm
n=62 Participants
This is the Intervention Arm of the LetSync Pilot Study to evaluate the feasibility and acceptability of the LetSync app intervention and associated protocols and procedures to inform a full-scale RCT. The Intervention Arm received the LetSync app to use through the 8-month trial.
Control Arm
n=72 Participants
This is the Control Arm of the LetSync Pilot Study to evaluate the feasibility and acceptability of the LetSync app intervention and associated protocols and procedures to inform a full-scale RCT. The Control Arm did not receive the LetSync app.
Self-reported Measure of Care Engagement
31.2 score on a scale
Standard Deviation 4.38
36.5 score on a scale
Standard Deviation 6.04

SECONDARY outcome

Timeframe: 8 months

Population: The sampling and analysis approaches differ: To be eligible, Index participants needed to enroll with their partner (i.e., Partner participants). However, for data analyses of the outcomes, we do not separate by Index vs. Partner individuals because we do not analyze individuals as "Index" vs. "Partner", i.e., we include all individuals who provide the outcomes data of interest. In this case, it is the Number of individual participants retained.

Between baseline (T0) and 8 months (T4), the investigators will collect feasibility-related data in terms of number of participants retained in the study from baseline to 8-month (T4) by condition. This is a feasibility and acceptability pilot trial, and one of the main outcomes is acceptability of the trial protocol and procedures, as indicated by participant retention rate, calculated as: The number of participants that remained in the trial divided by the total number of participants at the start of the trial.

Outcome measures

Outcome measures
Measure
Intervention Arm
n=69 Participants
This is the Intervention Arm of the LetSync Pilot Study to evaluate the feasibility and acceptability of the LetSync app intervention and associated protocols and procedures to inform a full-scale RCT. The Intervention Arm received the LetSync app to use through the 8-month trial.
Control Arm
n=74 Participants
This is the Control Arm of the LetSync Pilot Study to evaluate the feasibility and acceptability of the LetSync app intervention and associated protocols and procedures to inform a full-scale RCT. The Control Arm did not receive the LetSync app.
Number of Participants Retained
61 Participants
72 Participants

Adverse Events

Intervention Arm

Serious events: 0 serious events
Other events: 0 other events
Deaths: 0 deaths

Control Arm

Serious events: 0 serious events
Other events: 0 other events
Deaths: 0 deaths

Serious adverse events

Adverse event data not reported

Other adverse events

Adverse event data not reported

Additional Information

Judy Tan

Cedars-Sinai, UCSF

Phone: 310-423-1499

Results disclosure agreements

  • Principal investigator is a sponsor employee
  • Publication restrictions are in place