Trial Outcomes & Findings for Estradiol Effects on Alcohol Across the Menstrual Cycle (NCT NCT04595682)

NCT ID: NCT04595682

Last Updated: 2025-08-22

Results Overview

Attentional bias is measured by the visual dot-probe task and provides an implicit assessment of the rewarding properties of alcohol as indicated by the degree to which an acute dose of alcohol increases the drinker's attention to alcohol cues is measured. Subjects look at images on a screen and their attention to various images is measured. An alcohol-related image and a neutral control image are presented briefly side-by-side, on a computer screen. An eye tracker embedded into the monitor provides an unobtrusive measure of the duration (dwell time) that volunteers look at each image.

Recruitment status

COMPLETED

Study phase

NA

Target enrollment

100 participants

Primary outcome timeframe

1 day

Results posted on

2025-08-22

Participant Flow

For this study participants are randomly started in Late Follicular Phase (Day 12) or Early Follicular Phase (Day 5) based on when they are enrolled in the study and which day of their menstrual cycle they are on when enrolled. This order does not affect the treatment given to each participant in any way. Every participant is given a placebo, then alcohol.

Participant milestones

Participant milestones
Measure
Late Follicular Phase Visit First, Then Early Follicular Phase Visit
Participants in this group will track their menstrual cycle, provide daily saliva samples, and undergo two rounds of alcohol sensitivity testing (with both placebo and alcohol). beginning the study during their late follicular phase (approximately day 12) which is also when they begin their 35 days of consecutive daily data collection
Early Follicular Phase Visit First, Then Late Follicular Phase Visit
Participants in this group will track their menstrual cycle, provide daily saliva samples, and undergo two rounds of alcohol sensitivity testing (with both placebo and alcohol). beginning the study during their early follicular phase (approximately day 5) which is also when they begin their 35 days of consecutive daily data collection
Overall Study
STARTED
45
55
Overall Study
COMPLETED
45
55
Overall Study
NOT COMPLETED
0
0

Reasons for withdrawal

Withdrawal data not reported

Baseline Characteristics

Estradiol Effects on Alcohol Across the Menstrual Cycle

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
Study Participants
n=100 Participants
Participants in this group will track their menstrual cycle, provide daily saliva samples, and undergo two rounds of alcohol sensitivity testing (with both placebo and alcohol).
Age, Continuous
23.98 years
STANDARD_DEVIATION 3.47 • n=99 Participants
Sex: Female, Male
Female
100 Participants
n=99 Participants
Sex: Female, Male
Male
0 Participants
n=99 Participants
Race (NIH/OMB)
American Indian or Alaska Native
0 Participants
n=99 Participants
Race (NIH/OMB)
Asian
2 Participants
n=99 Participants
Race (NIH/OMB)
Native Hawaiian or Other Pacific Islander
0 Participants
n=99 Participants
Race (NIH/OMB)
Black or African American
8 Participants
n=99 Participants
Race (NIH/OMB)
White
80 Participants
n=99 Participants
Race (NIH/OMB)
More than one race
10 Participants
n=99 Participants
Race (NIH/OMB)
Unknown or Not Reported
0 Participants
n=99 Participants
Ethnicity (NIH/OMB)
Hispanic or Latino
8 Participants
n=99 Participants
Ethnicity (NIH/OMB)
Not Hispanic or Latino
92 Participants
n=99 Participants
Ethnicity (NIH/OMB)
Unknown or Not Reported
0 Participants
n=99 Participants
Region of Enrollment
United States
100 Participants
n=99 Participants

PRIMARY outcome

Timeframe: 1 day

Attentional bias is measured by the visual dot-probe task and provides an implicit assessment of the rewarding properties of alcohol as indicated by the degree to which an acute dose of alcohol increases the drinker's attention to alcohol cues is measured. Subjects look at images on a screen and their attention to various images is measured. An alcohol-related image and a neutral control image are presented briefly side-by-side, on a computer screen. An eye tracker embedded into the monitor provides an unobtrusive measure of the duration (dwell time) that volunteers look at each image.

Outcome measures

Outcome measures
Measure
Study Participants
n=100 Participants
Participants in this group will track their menstrual cycle, provide daily saliva samples, and undergo two rounds of alcohol sensitivity testing (with both placebo and alcohol).
Attentional Bias (Early Follicular Phase)
Alcohol
347.99 milliseconds
Standard Deviation 51.61
Attentional Bias (Early Follicular Phase)
Placebo
355.02 milliseconds
Standard Deviation 56.02

PRIMARY outcome

Timeframe: 1 day

Attentional bias is measured by the visual dot-probe task and provides an implicit assessment of the rewarding properties of alcohol as indicated by the degree to which an acute dose of alcohol increases the drinker's attention to alcohol cues is measured. Subjects look at images on a screen and their attention to various images is measured. An alcohol-related image and a neutral control image are presented briefly side-by-side, on a computer screen. An eye tracker embedded into the monitor provides an unobtrusive measure of the duration (dwell time) that volunteers look at each image.

Outcome measures

Outcome measures
Measure
Study Participants
n=100 Participants
Participants in this group will track their menstrual cycle, provide daily saliva samples, and undergo two rounds of alcohol sensitivity testing (with both placebo and alcohol).
Attentional Bias (Late Follicular Phase)
Alcohol
356.01 milliseconds
Standard Deviation 57.58
Attentional Bias (Late Follicular Phase)
Placebo
352.81 milliseconds
Standard Deviation 57.14

PRIMARY outcome

Timeframe: 1 day

Disinhibition wil be measured by the cued go/no-go task, which requires participants to respond quickly to go targets and inhibit responses to no-go targets. Participants complete 250 trials in which they are presented with a cue, followed by a target. A go target is green, and participants are instructed to press a button when presented with a go target. A no-go target is blue, and participants are instructed to do nothing when this appears. A go cue predicts a go target with 80% accuracy, whereas a no-go cue predicts a no-go target with 80% accuracy. The condition of interest is when a go cue is followed by a no-go target. The proportion reported is the proportion of trials under this condition in which participants press the button (expecting a go target but presented with a no-go target).

Outcome measures

Outcome measures
Measure
Study Participants
n=100 Participants
Participants in this group will track their menstrual cycle, provide daily saliva samples, and undergo two rounds of alcohol sensitivity testing (with both placebo and alcohol).
Disinhibition (Early Follicular Phase)
Alcohol
0.107 proportion of inhibition failures
Standard Deviation 0.12
Disinhibition (Early Follicular Phase)
Placebo
0.04 proportion of inhibition failures
Standard Deviation 0.06

PRIMARY outcome

Timeframe: 1 day

Disinhibition wil be measured by the cued go/no-go task, which requires participants to respond quickly to go targets and inhibit responses to no-go targets. Participants complete 250 trials in which they are presented with a cue, followed by a target. A go target is green, and participants are instructed to press a button when presented with a go target. A no-go target is blue, and participants are instructed to do nothing when this appears. A go cue predicts a go target with 80% accuracy, whereas a no-go cue predicts a no-go target with 80% accuracy. The condition of interest is when a go cue is followed by a no-go target. The proportion reported is the proportion of trials under this condition in which participants press the button (expecting a go target but presented with a no-go target).

Outcome measures

Outcome measures
Measure
Study Participants
n=100 Participants
Participants in this group will track their menstrual cycle, provide daily saliva samples, and undergo two rounds of alcohol sensitivity testing (with both placebo and alcohol).
Disinhibition (Late Follicular Phase)
Placebo
0.045 proportion of inhibition failures
Standard Deviation 0.06
Disinhibition (Late Follicular Phase)
Alcohol
0.134 proportion of inhibition failures
Standard Deviation 0.13

PRIMARY outcome

Timeframe: 1 day

a visual analog scale from 0-100, with 0 being not rewarding at all and 100 being very rewarding

Outcome measures

Outcome measures
Measure
Study Participants
n=100 Participants
Participants in this group will track their menstrual cycle, provide daily saliva samples, and undergo two rounds of alcohol sensitivity testing (with both placebo and alcohol).
Subjective Ratings of the Rewarding Effects of Alcohol (Early Follicular Phase)
Alcohol
56.99 score on a scale
Standard Deviation 25.61
Subjective Ratings of the Rewarding Effects of Alcohol (Early Follicular Phase)
Placebo
39.2 score on a scale
Standard Deviation 23.26

PRIMARY outcome

Timeframe: 1 day

a visual analog scale from 0-100, with 0 being not rewarding at all and 100 being very rewarding

Outcome measures

Outcome measures
Measure
Study Participants
n=100 Participants
Participants in this group will track their menstrual cycle, provide daily saliva samples, and undergo two rounds of alcohol sensitivity testing (with both placebo and alcohol).
Subjective Ratings of the Rewarding Effects of Alcohol (Late Follicular Phase)
Alcohol
60.17 score on a scale
Standard Deviation 22.23
Subjective Ratings of the Rewarding Effects of Alcohol (Late Follicular Phase)
Placebo
39.43 score on a scale
Standard Deviation 22.37

Adverse Events

Study Participants

Serious events: 0 serious events
Other events: 0 other events
Deaths: 0 deaths

Serious adverse events

Adverse event data not reported

Other adverse events

Adverse event data not reported

Additional Information

Dr. Mark Fillmore

University of Kentucky

Phone: 8592574728

Results disclosure agreements

  • Principal investigator is a sponsor employee
  • Publication restrictions are in place