Trial Outcomes & Findings for Omega-3 Fatty Acids for Smoking Cessation in Pregnancy (NCT NCT04417595)

NCT ID: NCT04417595

Last Updated: 2026-06-04

Results Overview

Gestational age at randomization will be determined on the basis of the last menstrual period and earliest ultrasound examination and will not be revised after being assigned a study group. Gestational age will be estimated from the last menstrual period (LMP) and adjusted for the first-trimester ultrasound. If a self-reported LMP is greater than 7 days from the calculated ultrasound LMP, the ultrasound will be used to assign gestational age.

Recruitment status

COMPLETED

Study phase

PHASE2

Target enrollment

237 participants

Primary outcome timeframe

At delivery

Results posted on

2026-06-04

Participant Flow

Participant milestones

Participant milestones
Measure
Fish Oil
Participants allocated to n-3 LCPUFA supplementation will be instructed to take four 1000 mg n-3 LCPUFA capsules (Metagenics™) daily. This will provide a total daily dose of 4000 mg n-3 LCPUFAs (2840 EPA and 1160 DHA). Fish Oil (Containing Omega-3 Acids): Fish Oil Supplement
Olive Oil
Oleic acid (olive oil) capsules have a similar texture, size, color, and consistency to EPA capsules. Participant will be instructed to take four 100mg olive oil capsules Olive Oil: Olive Oil Supplement
Overall Study
STARTED
117
120
Overall Study
COMPLETED
110
105
Overall Study
NOT COMPLETED
7
15

Reasons for withdrawal

Withdrawal data not reported

Baseline Characteristics

Omega-3 Fatty Acids for Smoking Cessation in Pregnancy

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
Olive Oil
n=120 Participants
Oleic acid (olive oil) capsules have a similar texture, size, color, and consistency to EPA capsules. Participant will be instructed to take four 100mg olive oil capsules Olive Oil: Olive Oil Supplement
Fish Oil
n=117 Participants
Participants allocated to n-3 LCPUFA supplementation will be instructed to take four 1000 mg n-3 LCPUFA capsules (Metagenics™) daily. This will provide a total daily dose of 4000 mg n-3 LCPUFAs (2840 EPA and 1160 DHA). Fish Oil (Containing Omega-3 Acids): Fish Oil Supplement
Total
n=237 Participants
Total of all reporting groups
Age, Continuous
30.67 years
STANDARD_DEVIATION 4.90 • n=9 Participants
29.81 years
STANDARD_DEVIATION 5.49 • n=27 Participants
30.25 years
STANDARD_DEVIATION 5.12 • n=267 Participants
Sex: Female, Male
Female
120 Participants
n=9 Participants
117 Participants
n=27 Participants
237 Participants
n=267 Participants
Sex: Female, Male
Male
0 Participants
n=9 Participants
0 Participants
n=27 Participants
0 Participants
n=267 Participants
Race (NIH/OMB)
American Indian or Alaska Native
0 Participants
n=9 Participants
1 Participants
n=27 Participants
1 Participants
n=267 Participants
Race (NIH/OMB)
Asian
0 Participants
n=9 Participants
0 Participants
n=27 Participants
0 Participants
n=267 Participants
Race (NIH/OMB)
Native Hawaiian or Other Pacific Islander
1 Participants
n=9 Participants
0 Participants
n=27 Participants
1 Participants
n=267 Participants
Race (NIH/OMB)
Black or African American
13 Participants
n=9 Participants
11 Participants
n=27 Participants
24 Participants
n=267 Participants
Race (NIH/OMB)
White
102 Participants
n=9 Participants
99 Participants
n=27 Participants
201 Participants
n=267 Participants
Race (NIH/OMB)
More than one race
2 Participants
n=9 Participants
4 Participants
n=27 Participants
6 Participants
n=267 Participants
Race (NIH/OMB)
Unknown or Not Reported
2 Participants
n=9 Participants
2 Participants
n=27 Participants
4 Participants
n=267 Participants
Region of Enrollment
United States
120 participants
n=9 Participants
117 participants
n=27 Participants
237 participants
n=267 Participants

PRIMARY outcome

Timeframe: At delivery

Population: Randomized participants

Gestational age at randomization will be determined on the basis of the last menstrual period and earliest ultrasound examination and will not be revised after being assigned a study group. Gestational age will be estimated from the last menstrual period (LMP) and adjusted for the first-trimester ultrasound. If a self-reported LMP is greater than 7 days from the calculated ultrasound LMP, the ultrasound will be used to assign gestational age.

Outcome measures

Outcome measures
Measure
Olive Oil
n=120 Participants
Oleic acid (olive oil) capsules have a similar texture, size, color, and consistency to EPA capsules. Participant will be instructed to take four 100mg olive oil capsules Olive Oil: Olive Oil Supplement
Fish Oil
n=117 Participants
Participants allocated to n-3 LCPUFA supplementation will be instructed to take four 1000 mg n-3 LCPUFA capsules (Metagenics™) daily. This will provide a total daily dose of 4000 mg n-3 LCPUFAs (2840 EPA and 1160 DHA). Fish Oil (Containing Omega-3 Acids): Fish Oil Supplement
Gestational Age at Delivery
38.33 weeks
Standard Deviation 1.90
38.09 weeks
Standard Deviation 1.60

PRIMARY outcome

Timeframe: At 12 weeks

Population: Analyzed participants who completed 12-week survey prior to delivery

Percentage change from baseline in cigarettes per day (CPD) at 12 weeks. The outcome will be determined based on participant self-report.

Outcome measures

Outcome measures
Measure
Olive Oil
n=81 Participants
Oleic acid (olive oil) capsules have a similar texture, size, color, and consistency to EPA capsules. Participant will be instructed to take four 100mg olive oil capsules Olive Oil: Olive Oil Supplement
Fish Oil
n=82 Participants
Participants allocated to n-3 LCPUFA supplementation will be instructed to take four 1000 mg n-3 LCPUFA capsules (Metagenics™) daily. This will provide a total daily dose of 4000 mg n-3 LCPUFAs (2840 EPA and 1160 DHA). Fish Oil (Containing Omega-3 Acids): Fish Oil Supplement
Change in Cigarettes Per Day
36.77 Percent change in CPD
Standard Deviation 83.97
35.24 Percent change in CPD
Standard Deviation 86.06

SECONDARY outcome

Timeframe: At delivery

Obtained from medical record review of delivery records by study personnel blinded to treatment allocation. This will be a categorical variable (yes or no). Outcomes will be adjudicated by study obstetricians blinded to treatment allocation.

Outcome measures

Outcome measures
Measure
Olive Oil
n=120 Participants
Oleic acid (olive oil) capsules have a similar texture, size, color, and consistency to EPA capsules. Participant will be instructed to take four 100mg olive oil capsules Olive Oil: Olive Oil Supplement
Fish Oil
n=117 Participants
Participants allocated to n-3 LCPUFA supplementation will be instructed to take four 1000 mg n-3 LCPUFA capsules (Metagenics™) daily. This will provide a total daily dose of 4000 mg n-3 LCPUFAs (2840 EPA and 1160 DHA). Fish Oil (Containing Omega-3 Acids): Fish Oil Supplement
Percent of Neonates With Fetal Death and Still Birth
1 Participants
0 Participants

SECONDARY outcome

Timeframe: At delivery

Population: Neonates

Obtained from medical record review of delivery records by study personnel blinded to treatment allocation. Birth weight will be collected in kilograms.

Outcome measures

Outcome measures
Measure
Olive Oil
n=120 Participants
Oleic acid (olive oil) capsules have a similar texture, size, color, and consistency to EPA capsules. Participant will be instructed to take four 100mg olive oil capsules Olive Oil: Olive Oil Supplement
Fish Oil
n=117 Participants
Participants allocated to n-3 LCPUFA supplementation will be instructed to take four 1000 mg n-3 LCPUFA capsules (Metagenics™) daily. This will provide a total daily dose of 4000 mg n-3 LCPUFAs (2840 EPA and 1160 DHA). Fish Oil (Containing Omega-3 Acids): Fish Oil Supplement
Individualized Birth Weight
111.39 ounces
Standard Deviation 20.54
108.69 ounces
Standard Deviation 19.98

SECONDARY outcome

Timeframe: At delivery

The Apgar score is a rapid assessment of a newborn's health at 1 and 5 minutes after birth, evaluating five criteria-Appearance (skin color), Pulse (heart rate), Grimace (reflexes), Activity (muscle tone), and Respiration (breathing). Each of the 5 criteria (APGAR) is scored 0, 1, or 2, totaling a minimum score of 0 and maximum of 10 points. A score of 7-10 is considered normal, indicating good to excellent. A score of 0-3 is critically low, indicating the need for immediate medical intervention. The score will be obtained from medical record review of delivery records by study personnel blinded to treatment allocation.

Outcome measures

Outcome measures
Measure
Olive Oil
n=120 Participants
Oleic acid (olive oil) capsules have a similar texture, size, color, and consistency to EPA capsules. Participant will be instructed to take four 100mg olive oil capsules Olive Oil: Olive Oil Supplement
Fish Oil
n=117 Participants
Participants allocated to n-3 LCPUFA supplementation will be instructed to take four 1000 mg n-3 LCPUFA capsules (Metagenics™) daily. This will provide a total daily dose of 4000 mg n-3 LCPUFAs (2840 EPA and 1160 DHA). Fish Oil (Containing Omega-3 Acids): Fish Oil Supplement
Apgar Score (5 Minute)
8.62 score on a scale (Apgar 5)
Standard Deviation 0.95
8.45 score on a scale (Apgar 5)
Standard Deviation 1.35

SECONDARY outcome

Timeframe: At delivery

Obtained from medical record review of delivery records by study personnel blinded to treatment allocation. This will be a categorical variable (yes or no). Outcomes will be adjudicated by study obstetricians blinded to treatment allocation.

Outcome measures

Outcome measures
Measure
Olive Oil
n=120 Participants
Oleic acid (olive oil) capsules have a similar texture, size, color, and consistency to EPA capsules. Participant will be instructed to take four 100mg olive oil capsules Olive Oil: Olive Oil Supplement
Fish Oil
n=117 Participants
Participants allocated to n-3 LCPUFA supplementation will be instructed to take four 1000 mg n-3 LCPUFA capsules (Metagenics™) daily. This will provide a total daily dose of 4000 mg n-3 LCPUFAs (2840 EPA and 1160 DHA). Fish Oil (Containing Omega-3 Acids): Fish Oil Supplement
Percent of Neonates With Intraventricular Hemorrhage
0 Participants
0 Participants

SECONDARY outcome

Timeframe: At delivery

Obtained from medical record review of delivery records by study personnel blinded to treatment allocation. This will be a categorical variable (yes or no). Outcomes will be adjudicated by study obstetricians blinded to treatment allocation.

Outcome measures

Outcome measures
Measure
Olive Oil
n=120 Participants
Oleic acid (olive oil) capsules have a similar texture, size, color, and consistency to EPA capsules. Participant will be instructed to take four 100mg olive oil capsules Olive Oil: Olive Oil Supplement
Fish Oil
n=117 Participants
Participants allocated to n-3 LCPUFA supplementation will be instructed to take four 1000 mg n-3 LCPUFA capsules (Metagenics™) daily. This will provide a total daily dose of 4000 mg n-3 LCPUFAs (2840 EPA and 1160 DHA). Fish Oil (Containing Omega-3 Acids): Fish Oil Supplement
Percent of Neonates With Neonatal Enterocolitis
0 Participants
0 Participants

SECONDARY outcome

Timeframe: At delivery

Obtained from medical record review of delivery records by study personnel blinded to treatment allocation. This will be a categorical variable (yes or no). Outcomes will be adjudicated by study obstetricians blinded to treatment allocation.

Outcome measures

Outcome measures
Measure
Olive Oil
n=120 Participants
Oleic acid (olive oil) capsules have a similar texture, size, color, and consistency to EPA capsules. Participant will be instructed to take four 100mg olive oil capsules Olive Oil: Olive Oil Supplement
Fish Oil
n=117 Participants
Participants allocated to n-3 LCPUFA supplementation will be instructed to take four 1000 mg n-3 LCPUFA capsules (Metagenics™) daily. This will provide a total daily dose of 4000 mg n-3 LCPUFAs (2840 EPA and 1160 DHA). Fish Oil (Containing Omega-3 Acids): Fish Oil Supplement
Percent of Neonates With a Congenital Abnormality
0 Participants
0 Participants

SECONDARY outcome

Timeframe: At delivery

Obtained from medical record review of delivery records by study personnel blinded to treatment allocation. This will be a categorical variable (yes or no). Outcomes will be adjudicated by study obstetricians blinded to treatment allocation.

Outcome measures

Outcome measures
Measure
Olive Oil
n=120 Participants
Oleic acid (olive oil) capsules have a similar texture, size, color, and consistency to EPA capsules. Participant will be instructed to take four 100mg olive oil capsules Olive Oil: Olive Oil Supplement
Fish Oil
n=117 Participants
Participants allocated to n-3 LCPUFA supplementation will be instructed to take four 1000 mg n-3 LCPUFA capsules (Metagenics™) daily. This will provide a total daily dose of 4000 mg n-3 LCPUFAs (2840 EPA and 1160 DHA). Fish Oil (Containing Omega-3 Acids): Fish Oil Supplement
Percent of Neonates With Neonatal Respiratory Distress
0 Participants
0 Participants

SECONDARY outcome

Timeframe: At delivery

Obtained from medical record review of delivery records by study personnel blinded to treatment allocation. This will be a categorical variable (yes or no). Outcomes will be adjudicated by study obstetricians blinded to treatment allocation.

Outcome measures

Outcome measures
Measure
Olive Oil
n=120 Participants
Oleic acid (olive oil) capsules have a similar texture, size, color, and consistency to EPA capsules. Participant will be instructed to take four 100mg olive oil capsules Olive Oil: Olive Oil Supplement
Fish Oil
n=117 Participants
Participants allocated to n-3 LCPUFA supplementation will be instructed to take four 1000 mg n-3 LCPUFA capsules (Metagenics™) daily. This will provide a total daily dose of 4000 mg n-3 LCPUFAs (2840 EPA and 1160 DHA). Fish Oil (Containing Omega-3 Acids): Fish Oil Supplement
Percent of Participants With Maternal Mortality
0 Participants
0 Participants

SECONDARY outcome

Timeframe: At delivery

Obtained from medical record review of delivery records by study personnel blinded to treatment allocation. This will be a categorical variable (yes or no). Outcomes will be adjudicated by study obstetricians blinded to treatment allocation.

Outcome measures

Outcome measures
Measure
Olive Oil
n=120 Participants
Oleic acid (olive oil) capsules have a similar texture, size, color, and consistency to EPA capsules. Participant will be instructed to take four 100mg olive oil capsules Olive Oil: Olive Oil Supplement
Fish Oil
n=117 Participants
Participants allocated to n-3 LCPUFA supplementation will be instructed to take four 1000 mg n-3 LCPUFA capsules (Metagenics™) daily. This will provide a total daily dose of 4000 mg n-3 LCPUFAs (2840 EPA and 1160 DHA). Fish Oil (Containing Omega-3 Acids): Fish Oil Supplement
Percent of Participants Requiring Cesarean Delivery
36 Participants
45 Participants

SECONDARY outcome

Timeframe: At delivery

Obtained from medical record review of delivery records by study personnel blinded to treatment allocation. This will be a categorical variable (yes or no). Outcomes will be adjudicated by study obstetricians blinded to treatment allocation.

Outcome measures

Outcome measures
Measure
Olive Oil
n=120 Participants
Oleic acid (olive oil) capsules have a similar texture, size, color, and consistency to EPA capsules. Participant will be instructed to take four 100mg olive oil capsules Olive Oil: Olive Oil Supplement
Fish Oil
n=117 Participants
Participants allocated to n-3 LCPUFA supplementation will be instructed to take four 1000 mg n-3 LCPUFA capsules (Metagenics™) daily. This will provide a total daily dose of 4000 mg n-3 LCPUFAs (2840 EPA and 1160 DHA). Fish Oil (Containing Omega-3 Acids): Fish Oil Supplement
Percent of Participants Developing Hypertension in Pregnancy
0 Participants
0 Participants

SECONDARY outcome

Timeframe: At 12 weeks

Population: Analyzed participants who completed 12-week survey prior to delivery

Point prevalence abstinence at 12 weeks will be based on self-reported smoking cessation and biochemically confirmed by end-expired carbon monoxide (end-expired CO less than 4 ppm)

Outcome measures

Outcome measures
Measure
Olive Oil
n=81 Participants
Oleic acid (olive oil) capsules have a similar texture, size, color, and consistency to EPA capsules. Participant will be instructed to take four 100mg olive oil capsules Olive Oil: Olive Oil Supplement
Fish Oil
n=82 Participants
Participants allocated to n-3 LCPUFA supplementation will be instructed to take four 1000 mg n-3 LCPUFA capsules (Metagenics™) daily. This will provide a total daily dose of 4000 mg n-3 LCPUFAs (2840 EPA and 1160 DHA). Fish Oil (Containing Omega-3 Acids): Fish Oil Supplement
Percent of Participants Reaching Point Prevalence Abstinence
2 Participants
11 Participants

SECONDARY outcome

Timeframe: At 12 weeks

Population: Percentage of participants with a decrease in Fagerström Test for Nicotine Dependence (summation score is lower at 12 weeks compared to baseline).

Changes in nicotine dependence based on changes in the Fagerström Test for Nicotine Dependence. The Fagerström Test for Nicotine Dependence is a 6-question scale used to assess the intensity of physical addiction to nicotine. Minimum score is 0 which indicates very low dependence and maximum score is 10 which indicates very high dependence. Each question allows for different categorical responses which are assigned a point value. The points are summed to create a summary score. The score at clinical visit 2 (12 weeks after the baseline/randomization visit) are compared to those at clinical visit 1 (baseline/randomization visit). Differences in score were categorized as an increase in score (increasing dependence), no change in score, or a decrease in score (decreasing dependence),

Outcome measures

Outcome measures
Measure
Olive Oil
n=120 Participants
Oleic acid (olive oil) capsules have a similar texture, size, color, and consistency to EPA capsules. Participant will be instructed to take four 100mg olive oil capsules Olive Oil: Olive Oil Supplement
Fish Oil
n=117 Participants
Participants allocated to n-3 LCPUFA supplementation will be instructed to take four 1000 mg n-3 LCPUFA capsules (Metagenics™) daily. This will provide a total daily dose of 4000 mg n-3 LCPUFAs (2840 EPA and 1160 DHA). Fish Oil (Containing Omega-3 Acids): Fish Oil Supplement
Percent of Patients With a Decrease in Self-reported Nicotine Dependence
50 Participants
52 Participants

Adverse Events

Fish Oil

Serious events: 0 serious events
Other events: 0 other events
Deaths: 0 deaths

Olive Oil

Serious events: 1 serious events
Other events: 0 other events
Deaths: 0 deaths

Serious adverse events

Serious adverse events
Measure
Fish Oil
n=117 participants at risk
Participants allocated to n-3 LCPUFA supplementation will be instructed to take four 1000 mg n-3 LCPUFA capsules (Metagenics™) daily. This will provide a total daily dose of 4000 mg n-3 LCPUFAs (2840 EPA and 1160 DHA). Fish Oil (Containing Omega-3 Acids): Fish Oil Supplement
Olive Oil
n=120 participants at risk
Oleic acid (olive oil) capsules have a similar texture, size, color, and consistency to EPA capsules. Participant will be instructed to take four 100mg olive oil capsules Olive Oil: Olive Oil Supplement
Pregnancy, puerperium and perinatal conditions
Fetal Demise
0.00%
0/117 • Approximately 9 months from start of study to end of study follow-up
Adverse events presented in this section include maternal adverse events. Adverse events for neonates are reported as secondary outcomes
0.83%
1/120 • Number of events 1 • Approximately 9 months from start of study to end of study follow-up
Adverse events presented in this section include maternal adverse events. Adverse events for neonates are reported as secondary outcomes

Other adverse events

Adverse event data not reported

Additional Information

Dr. Harvey Murff

Vanderbilt University Medical Center

Phone: 6152253788

Results disclosure agreements

  • Principal investigator is a sponsor employee
  • Publication restrictions are in place