Trial Outcomes & Findings for PH94B in the Treatment of Adjustment Disorder With Anxiety (NCT NCT04404192)

NCT ID: NCT04404192

Last Updated: 2026-02-05

Results Overview

Least squares mean change from baseline to Visit 6 in anxiety level as measured by the HAM-A at the end of the 4-week treatment period. The HAM-A questionnaire consists of 14 items, each scored on a 5-point scale (0="Not present", 1="Mild", 2="Moderate", 3="Severe", 4="Very severe. The HAM-A total score is calculated as the sum of the 14 individual scores, ranging from 0 to 56 where \<17 indicates mild severity, 18-24 mild to moderate severity, and 25-30 moderate to severe. Higher total scores indicate more severe disease.

Recruitment status

COMPLETED

Study phase

PHASE2

Target enrollment

41 participants

Primary outcome timeframe

28 days

Results posted on

2026-02-05

Participant Flow

Participant milestones

Participant milestones
Measure
PH94B
PH94B Nasal Spray - 3.2 micrograms four times a day for 28 days
Placebo
Placebo Nasal Spray - four times a day for 28 days
Overall Study
STARTED
19
22
Overall Study
COMPLETED
15
19
Overall Study
NOT COMPLETED
4
3

Reasons for withdrawal

Reasons for withdrawal
Measure
PH94B
PH94B Nasal Spray - 3.2 micrograms four times a day for 28 days
Placebo
Placebo Nasal Spray - four times a day for 28 days
Overall Study
Adverse Event
1
0
Overall Study
Lost to Follow-up
1
1
Overall Study
Withdrawal by Subject
1
2
Overall Study
Missed 2 or more consecutive visits during 4-week treatment period
1
0

Baseline Characteristics

PH94B in the Treatment of Adjustment Disorder With Anxiety

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
PH94B
n=19 Participants
PH94B Nasal Spray - 3.2 micrograms four times a day for 28 days
Placebo
n=22 Participants
Placebo Nasal Spray - four times a day for 28 days
Total
n=41 Participants
Total of all reporting groups
Race (NIH/OMB)
Native Hawaiian or Other Pacific Islander
0 Participants
n=41 Participants
0 Participants
n=1581 Participants
0 Participants
n=4626 Participants
Race (NIH/OMB)
Black or African American
1 Participants
n=41 Participants
1 Participants
n=1581 Participants
2 Participants
n=4626 Participants
Race (NIH/OMB)
White
17 Participants
n=41 Participants
19 Participants
n=1581 Participants
36 Participants
n=4626 Participants
Race (NIH/OMB)
More than one race
0 Participants
n=41 Participants
0 Participants
n=1581 Participants
0 Participants
n=4626 Participants
Race (NIH/OMB)
Unknown or Not Reported
0 Participants
n=41 Participants
1 Participants
n=1581 Participants
1 Participants
n=4626 Participants
Age, Categorical
<=18 years
0 Participants
n=41 Participants
0 Participants
n=1581 Participants
0 Participants
n=4626 Participants
Age, Categorical
Between 18 and 65 years
19 Participants
n=41 Participants
20 Participants
n=1581 Participants
39 Participants
n=4626 Participants
Age, Categorical
>=65 years
0 Participants
n=41 Participants
2 Participants
n=1581 Participants
2 Participants
n=4626 Participants
Age, Continuous
35.7 years
STANDARD_DEVIATION 12.74 • n=41 Participants
41.5 years
STANDARD_DEVIATION 13.00 • n=1581 Participants
38.8 years
STANDARD_DEVIATION 13.06 • n=4626 Participants
Sex: Female, Male
Female
6 Participants
n=41 Participants
11 Participants
n=1581 Participants
17 Participants
n=4626 Participants
Sex: Female, Male
Male
13 Participants
n=41 Participants
11 Participants
n=1581 Participants
24 Participants
n=4626 Participants
Ethnicity (NIH/OMB)
Hispanic or Latino
4 Participants
n=41 Participants
4 Participants
n=1581 Participants
8 Participants
n=4626 Participants
Ethnicity (NIH/OMB)
Not Hispanic or Latino
15 Participants
n=41 Participants
18 Participants
n=1581 Participants
33 Participants
n=4626 Participants
Ethnicity (NIH/OMB)
Unknown or Not Reported
0 Participants
n=41 Participants
0 Participants
n=1581 Participants
0 Participants
n=4626 Participants
Race (NIH/OMB)
American Indian or Alaska Native
1 Participants
n=41 Participants
0 Participants
n=1581 Participants
1 Participants
n=4626 Participants
Race (NIH/OMB)
Asian
0 Participants
n=41 Participants
1 Participants
n=1581 Participants
1 Participants
n=4626 Participants
Region of Enrollment
United States
19 participants
n=41 Participants
22 participants
n=1581 Participants
41 participants
n=4626 Participants
Hamilton Anxiety Scale (HAM-A) Total Score
23.7 score on a scale
STANDARD_DEVIATION 3.32 • n=41 Participants
23.1 score on a scale
STANDARD_DEVIATION 2.64 • n=1581 Participants
23.4 score on a scale
STANDARD_DEVIATION 2.95 • n=4626 Participants

PRIMARY outcome

Timeframe: 28 days

Least squares mean change from baseline to Visit 6 in anxiety level as measured by the HAM-A at the end of the 4-week treatment period. The HAM-A questionnaire consists of 14 items, each scored on a 5-point scale (0="Not present", 1="Mild", 2="Moderate", 3="Severe", 4="Very severe. The HAM-A total score is calculated as the sum of the 14 individual scores, ranging from 0 to 56 where \<17 indicates mild severity, 18-24 mild to moderate severity, and 25-30 moderate to severe. Higher total scores indicate more severe disease.

Outcome measures

Outcome measures
Measure
PH94B
n=19 Participants
PH94B Nasal Spray - 3.2 micrograms four times a day for 28 days
Placebo
n=22 Participants
Placebo Nasal Spray - four times a day for 28 days
Hamilton Anxiety Scale (HAM-A) Score
-12.5 score on a scale
Standard Error 1.31
-12.6 score on a scale
Standard Error 1.22

SECONDARY outcome

Timeframe: 28 days

The CGI-I scale is a clinician-rated scale to assess illness improvement. The CGI-I scale includes one item scored from 1 (Very much less anxious) to 7 (Very much more anxious) with 4 being no change. Subjects are considered CGI-I responders with scores of 1 (Very much less anxious) or 2 (Much less anxious).

Outcome measures

Outcome measures
Measure
PH94B
n=19 Participants
PH94B Nasal Spray - 3.2 micrograms four times a day for 28 days
Placebo
n=22 Participants
Placebo Nasal Spray - four times a day for 28 days
Percentage of Participants With Clinical Global Impression - Improvement Rating (CGI-I) Response
12 Participants
16 Participants

SECONDARY outcome

Timeframe: 28 days

The PGI-C is a self-administered instrument that measures change in subjects' overall improvement with treatment on a scale where 1= "very much improved" and 7= "very much worse". Values for comparison were the proportion of "responders" in each group, defined as subjects receiving scores of 1 (very much improved) or 2 (much improved) at the end of treatment.

Outcome measures

Outcome measures
Measure
PH94B
n=19 Participants
PH94B Nasal Spray - 3.2 micrograms four times a day for 28 days
Placebo
n=22 Participants
Placebo Nasal Spray - four times a day for 28 days
Percentage of Participants With Patient Global Impression of Change (PGI-C) Response
8 Participants
14 Participants

SECONDARY outcome

Timeframe: 28 days

Least squares mean change from baseline to Visit 6 in ADNM total score. THE ADNM consists of 18-item stressful life checklist and a list of 20 statements about which reactions these types of events can trigger. Subjects are asked to indicate of how often the statements apply on a 4-point scale (1= "never", 2= "rarely", 3= "sometimes", 4= "often"). ADNM total score is calculated as the sum of the 20 frequency scores. Higher total scores indicate more severe disease.

Outcome measures

Outcome measures
Measure
PH94B
n=19 Participants
PH94B Nasal Spray - 3.2 micrograms four times a day for 28 days
Placebo
n=22 Participants
Placebo Nasal Spray - four times a day for 28 days
Adjustment Disorder New Module Scale (ADNM) Score
-9.9 score on a scale
Standard Error 2.19
-12.1 score on a scale
Standard Error 2.13

SECONDARY outcome

Timeframe: 28 days

Least squares mean change from baseline to Visit 6 in IADQ total score. The preoccupation, failure to adapt, and functional impairment subscale items are scored on a 5-point scale (0="Not at all", 1="A little bit", 2="Moderately", 3="Quite a bit", and 4="Extremely"). The IADQ total score is calculated as the sum of the 6 preoccupation and failure to adapt items. Higher total scores indicate more severe disease.

Outcome measures

Outcome measures
Measure
PH94B
n=19 Participants
PH94B Nasal Spray - 3.2 micrograms four times a day for 28 days
Placebo
n=22 Participants
Placebo Nasal Spray - four times a day for 28 days
International Adjustment Disorder Questionnaire (IADQ) Score
-9.0 score on a scale
Standard Error 1.49
-11.4 score on a scale
Standard Error 1.45

Adverse Events

PH94B

Serious events: 0 serious events
Other events: 11 other events
Deaths: 0 deaths

Placebo

Serious events: 0 serious events
Other events: 11 other events
Deaths: 0 deaths

Serious adverse events

Adverse event data not reported

Other adverse events

Other adverse events
Measure
PH94B
n=19 participants at risk
PH94B Nasal Spray - 3.2 micrograms four times a day for 28 days
Placebo
n=22 participants at risk
Placebo Nasal Spray - four times a day for 28 days
Cardiac disorders
Palpitations
5.3%
1/19 • 28 days
0.00%
0/22 • 28 days
Ear and labyrinth disorders
Ear pain
0.00%
0/19 • 28 days
4.5%
1/22 • 28 days
Immune system disorders
Seasonal allergy
0.00%
0/19 • 28 days
4.5%
1/22 • 28 days
Nervous system disorders
Dizziness
5.3%
1/19 • 28 days
4.5%
1/22 • 28 days
Nervous system disorders
Disturbance in attention
5.3%
1/19 • 28 days
0.00%
0/22 • 28 days
Gastrointestinal disorders
Nausea
5.3%
1/19 • 28 days
4.5%
1/22 • 28 days
Respiratory, thoracic and mediastinal disorders
Nasal Discomfort
5.3%
1/19 • 28 days
0.00%
0/22 • 28 days
Respiratory, thoracic and mediastinal disorders
Nasal Dryness
5.3%
1/19 • 28 days
0.00%
0/22 • 28 days
General disorders
Fatigue
5.3%
1/19 • 28 days
4.5%
1/22 • 28 days
Gastrointestinal disorders
Diarrhoea
0.00%
0/19 • 28 days
4.5%
1/22 • 28 days
Gastrointestinal disorders
Salivary hypersecretion
0.00%
0/19 • 28 days
4.5%
1/22 • 28 days
Psychiatric disorders
Insomnia
5.3%
1/19 • 28 days
4.5%
1/22 • 28 days
Psychiatric disorders
Abnormal dreams
0.00%
0/19 • 28 days
4.5%
1/22 • 28 days
Psychiatric disorders
Change in sustained attention
0.00%
0/19 • 28 days
4.5%
1/22 • 28 days
Respiratory, thoracic and mediastinal disorders
Epistaxis
0.00%
0/19 • 28 days
4.5%
1/22 • 28 days
Respiratory, thoracic and mediastinal disorders
Oropharyngeal pain
0.00%
0/19 • 28 days
4.5%
1/22 • 28 days
Infections and infestations
COVID-19
5.3%
1/19 • 28 days
4.5%
1/22 • 28 days
Infections and infestations
Gastroenteritis
5.3%
1/19 • 28 days
0.00%
0/22 • 28 days
Eye disorders
Photophobia
0.00%
0/19 • 28 days
4.5%
1/22 • 28 days
Eye disorders
Vision blurred
5.3%
1/19 • 28 days
0.00%
0/22 • 28 days
Injury, poisoning and procedural complications
Contusion
0.00%
0/19 • 28 days
4.5%
1/22 • 28 days
Injury, poisoning and procedural complications
Oral contusion
0.00%
0/19 • 28 days
4.5%
1/22 • 28 days
Injury, poisoning and procedural complications
Rib fracture
5.3%
1/19 • 28 days
0.00%
0/22 • 28 days
Injury, poisoning and procedural complications
Skin laceration
0.00%
0/19 • 28 days
4.5%
1/22 • 28 days
Musculoskeletal and connective tissue disorders
Back pain
0.00%
0/19 • 28 days
4.5%
1/22 • 28 days
Musculoskeletal and connective tissue disorders
Myalgia
0.00%
0/19 • 28 days
4.5%
1/22 • 28 days
Skin and subcutaneous tissue disorders
Dermatitis
0.00%
0/19 • 28 days
4.5%
1/22 • 28 days
Skin and subcutaneous tissue disorders
Dermatitis contact
5.3%
1/19 • 28 days
0.00%
0/22 • 28 days
Nervous system disorders
Headache
15.8%
3/19 • 28 days
9.1%
2/22 • 28 days

Additional Information

Clinical Studies

Vistagen Therapeutics, Inc.

Phone: 650-577-3693

Results disclosure agreements

  • Principal investigator is a sponsor employee
  • Publication restrictions are in place