Trial Outcomes & Findings for Effects of Ocrelizumab on B-cell Tolerance Defect in Relapsing Multiple Sclerosis (NCT NCT04261790)

NCT ID: NCT04261790

Last Updated: 2025-02-17

Results Overview

By assessing the antibodies produced by isolated B-cells, changes in the frequencies (percentage) of polyreactive, and anti-nuclear clones of new emigrant/transitional and mature naive B-cells will be determined.

Recruitment status

COMPLETED

Study phase

PHASE4

Target enrollment

10 participants

Primary outcome timeframe

Baseline and 18-24 months

Results posted on

2025-02-17

Participant Flow

Participant milestones

Participant milestones
Measure
Ocrelizumab
Patients will be treated with two courses of ocrelizumab (Ocrevus) for one year and then will stop the medication and will be monitored for the return of the disease activity. Those who experience the return of the disease activity can go back on the medication. Ocrelizumab: Patients will be treated with two courses of ocrelizumab (Ocrevus) 600mg/course, for one year and then will stop the medication and will be monitored for the return of the disease activity. Those who experience the return of the disease activity can go back on the medication.
Overall Study
STARTED
10
Overall Study
COMPLETED
10
Overall Study
NOT COMPLETED
0

Reasons for withdrawal

Withdrawal data not reported

Baseline Characteristics

Effects of Ocrelizumab on B-cell Tolerance Defect in Relapsing Multiple Sclerosis

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
Ocrelizumab
n=10 Participants
Patients will be treated with two courses of ocrelizumab (Ocrevus) for one year and then will stop the medication and will be monitored for the return of the disease activity. Those who experience the return of the disease activity can go back on the medication. Ocrelizumab: Patients will be treated with two courses of ocrelizumab (Ocrevus) 600mg/course, for one year and then will stop the medication and will be monitored for the return of the disease activity. Those who experience the return of the disease activity can go back on the medication.
Age, Continuous
36 years
STANDARD_DEVIATION 8.5 • n=99 Participants
Sex: Female, Male
Female
6 Participants
n=99 Participants
Sex: Female, Male
Male
4 Participants
n=99 Participants
Ethnicity (NIH/OMB)
Hispanic or Latino
0 Participants
n=99 Participants
Ethnicity (NIH/OMB)
Not Hispanic or Latino
10 Participants
n=99 Participants
Ethnicity (NIH/OMB)
Unknown or Not Reported
0 Participants
n=99 Participants
Race (NIH/OMB)
American Indian or Alaska Native
0 Participants
n=99 Participants
Race (NIH/OMB)
Asian
1 Participants
n=99 Participants
Race (NIH/OMB)
Native Hawaiian or Other Pacific Islander
0 Participants
n=99 Participants
Race (NIH/OMB)
Black or African American
2 Participants
n=99 Participants
Race (NIH/OMB)
White
6 Participants
n=99 Participants
Race (NIH/OMB)
More than one race
1 Participants
n=99 Participants
Race (NIH/OMB)
Unknown or Not Reported
0 Participants
n=99 Participants
Disease Duration
2.8 years
STANDARD_DEVIATION 7.0 • n=99 Participants
Baseline Expanded Disability Status Scale (EDSS)
1.0 units on a scale
n=99 Participants
Disease-modifying Therapy (DMT) at Screening
No DMT
8 Participants
n=99 Participants
Disease-modifying Therapy (DMT) at Screening
Glatiramer acetate
2 Participants
n=99 Participants

PRIMARY outcome

Timeframe: Baseline and 18-24 months

Population: Data were not collected. None of the antibodies from the immune cells were able to cloned for analysis and no data were collected from the lab samples.

By assessing the antibodies produced by isolated B-cells, changes in the frequencies (percentage) of polyreactive, and anti-nuclear clones of new emigrant/transitional and mature naive B-cells will be determined.

Outcome measures

Outcome data not reported

PRIMARY outcome

Timeframe: Baseline and 18-24 months

Population: Data were not collected. None of the antibodies from the immune cells were able to cloned for analysis and no data were collected from the lab samples.

Change in the frequency (percentage) of different B-cell subpopulation (assessed by flow cytometry) before and after treatment with ocrelizumab.

Outcome measures

Outcome data not reported

PRIMARY outcome

Timeframe: Baseline and 18-24 months

Population: Data were not collected. None of the antibodies from the immune cells were able to cloned for analysis and no data were collected from the lab samples.

Change in the frequency (percentage) of different T-cells subpopulation (assessed by flow cytometry) before and after treatment with ocrelizumab.

Outcome measures

Outcome data not reported

PRIMARY outcome

Timeframe: Baseline and 18-24 months

Population: Data were not collected. None of the antibodies from the immune cells were able to cloned for analysis and no data were collected from the lab samples.

Pro inflammatory cytokines, produced by Tregs and other T cell subsets after activating peripheral blood mononucleated cell with phorbol-12-myristate-13-acetate (PMA) and ionomycin will be measured by enzyme-linked immunosorbent assay.

Outcome measures

Outcome data not reported

PRIMARY outcome

Timeframe: Baseline and 18-24 months

Population: Data were not collected. None of the antibodies from the immune cells were able to cloned for analysis and no data were collected from the lab samples.

Anti-inflammatory cytokines, produced by Tregs and other T cell subsets after activating peripheral blood mononucleated cell with phorbol-12-myristate-13-acetate (PMA) and ionomycin will be measured by enzyme-linked immunosorbent assay

Outcome measures

Outcome data not reported

SECONDARY outcome

Timeframe: Up to 30 months

Patients with the return of disease activity after the third month post-first-infusion, objectively demonstrated by development of new T2 hyperintense lesions or Gd-enhancing lesions on the MRI or a clinical relapse that is confirmed with an objective change in the neurological examination.

Outcome measures

Outcome measures
Measure
Ocrelizumab
n=10 Participants
Ocrelizumab group
Patients With Return of Disease Activity
Participants who experienced the return of disease activity during the 30-month trial
0 Participants
Patients With Return of Disease Activity
Participants who did not experience the return of disease activity during the 30-month trial
10 Participants

SECONDARY outcome

Timeframe: Screening visit and month 30 visit

EDSS scores range from 0 to 10, with 0.5 steps. The higher the score, the worse the MS-related disability.

Outcome measures

Outcome measures
Measure
Ocrelizumab
n=10 Participants
Ocrelizumab group
Change in Disability as Assessed by Expanded Disability Status Scale (EDSS)
-0.5 units on a scale
Standard Deviation 6.0

SECONDARY outcome

Timeframe: Screening visit and month 30 visit

T-score (standardized scores with a mean of 50 and a standard deviation (SD) of 10). A higher Neuro-QoL T-score represents more of the concept being measured. There is no specific upper or lower limit for this scoring

Outcome measures

Outcome measures
Measure
Ocrelizumab
n=10 Participants
Ocrelizumab group
Change in Quality of Life as Assessed by Neuro-QoL Fatigue T-score
2.6 units on a scale
Standard Deviation 5.0

Adverse Events

Ocrelizumab

Serious events: 0 serious events
Other events: 7 other events
Deaths: 0 deaths

Serious adverse events

Adverse event data not reported

Other adverse events

Other adverse events
Measure
Ocrelizumab
n=10 participants at risk
Ocrelizumab group
Nervous system disorders
Headache
30.0%
3/10 • Number of events 3 • Participants received two courses of treatment with ocrelizumab six months apart. Adverse event data were collected up to 24 months after the second (last) ocrelizumab infusion.
Respiratory, thoracic and mediastinal disorders
Cough
20.0%
2/10 • Number of events 2 • Participants received two courses of treatment with ocrelizumab six months apart. Adverse event data were collected up to 24 months after the second (last) ocrelizumab infusion.
Cardiac disorders
Chest discomfort
10.0%
1/10 • Number of events 1 • Participants received two courses of treatment with ocrelizumab six months apart. Adverse event data were collected up to 24 months after the second (last) ocrelizumab infusion.
Vascular disorders
Blood pressure fluctuation
10.0%
1/10 • Number of events 1 • Participants received two courses of treatment with ocrelizumab six months apart. Adverse event data were collected up to 24 months after the second (last) ocrelizumab infusion.

Additional Information

Bardia Nourbakhsh

Johns Hopkins Universtiy

Phone: 410-614-1522

Results disclosure agreements

  • Principal investigator is a sponsor employee
  • Publication restrictions are in place