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NMR Metabolomics Study of Follicular Fluid of Oncological Patients

NCT04101981 · Status: COMPLETED · Type: OBSERVATIONAL · Enrollment: 20

Last updated 2020-11-24

No results posted yet for this study

Summary

Purpose: The purpose of this study was to evaluate the possible application of metabolomics to identify follicular fluid changes in cancer patients undergoing fertility preservation. Although metabolomics have been applied already in cancer studies, this is the first application on follicular fluid of cancer patients.

Methods: The investigators selected for the study 10 patients with breast cancer and lymphoma who resorted to oocyte cryopreservation to preserve fertility and 10 healthy women undergoing in vitro fertilization treatments. Follicular fluid was collected at the time of oocytes retrieval. Metabolomic analysis of follicular fluids was performed by 1H NMR spectroscopy in combination with multivariate analysis to interpret the spectral data. Univariate statistical analysis was applied to find correlations between patients' features and metabolites identified by NMR.

Conditions

  • Woman With Cancer Resorting Fertility Preservation

Interventions

PROCEDURE

oocytes retrieval

The oocytes retrieval was performed after 34-36 hours from hCG administration . The collection of cumulus-oocyte complexes and FFs were performed via transvaginal ultrasound-guided aspiration with a needle of 18 gauge of diameter. The three major diameter follicles were identified by intraoperative ultrasound and FF of each follicle was collected in its own test tube.

Sponsors & Collaborators

  • San Carlo Public Hospital, Potenza, Italy

    lead OTHER

Principal Investigators

  • Maria Antonietta MACM Castiglione Morelli, Professor · University of Basilicata studies

  • Assunta Iuliano, MD · San Carlo Public Hospital, Potenza

Eligibility

Min Age
18 Years
Max Age
38 Years
Sex
FEMALE
Healthy Volunteers
No

Timeline & Regulatory

Start
2016-05-01
Primary Completion
2017-12-31
Completion
2018-05-31
FDA Drug
Yes

Countries

  • Italy

Study Locations

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Read the full study record

This page highlights key information. For complete eligibility criteria, study locations, investigator contacts, and the full protocol, visit the original record on ClinicalTrials.gov.

View NCT04101981 on ClinicalTrials.gov