Trial Outcomes & Findings for Timing of Sodium Intake and Nocturnal Sodium Excretion and Blood Pressure in Obese African Americans (NCT NCT04021355)

NCT ID: NCT04021355

Last Updated: 2026-08-20

Results Overview

Systolic blood pressure measured during sleep by 24-hour ambulatory blood pressure monitor starting on the 8th day of consuming the standarized diet plus chicken-based broth

Recruitment status

COMPLETED

Study phase

NA

Target enrollment

61 participants

Primary outcome timeframe

On the 8th day of consuming the standarized diet plus chicken-based broth

Results posted on

2026-08-20

Participant Flow

A total of 68 participants were screened for eligibilty in the study between November 2020 to April 2025.

A total of 7 participants who were screened decided not to participate before randomization because of inability to adhere to time requirements of the study or the study diets. This left 61 participants who were enrolled in the study.

Participant milestones

Participant milestones
Measure
Morning Sodium Intervention, Then Evening Sodium Intervention
The study was conducted as a randomized, crossover feeding trial. During the first seven days of this period (run-in period), participants consumed only the study meals to standardize sodium intake. During the final nine days of this period (intervention period), participants continued to eat study meals and additionally consumed a chicken-based broth containing a total of 2 grams of sodium with breakfast (morning sodium). After a 4-week washout period, participants crossed over to the second study arm in which they consumed the study meals for seven days, and then consumed the study meals + a chicken-based broth containing a total of 2 grams of sodium with dinner for nine days (evening sodium).
Evening Sodium Intervention, Followed by Morning Sodium Intervention
The study was conducted as a randomized, crossover feeding trial. During the first seven days of this period (run-in period), participants consumed only the study meals to standardize sodium intake. During the final nine days of this period (intervention period), participants continued to eat study meals and additionally consumed a chicken-based broth containing a total of 2 grams of sodium with dinner (evening sodium). After a 4-week washout period, participants crossed over to the second study arm in which they consumed the study meals for seven days, and then consumed the study meals + a chicken-based broth containing a total of 2 grams of sodium with breakfast for nine days (morning sodium).
Second Intervention (16 days)
NOT COMPLETED
0
0
First Intervention (16 days)
STARTED
35
26
First Intervention (16 days)
COMPLETED
30
23
First Intervention (16 days)
NOT COMPLETED
5
3
Washout (4 weeks)
STARTED
30
23
Washout (4 weeks)
COMPLETED
27
21
Washout (4 weeks)
NOT COMPLETED
3
2
Second Intervention (16 days)
STARTED
27
21
Second Intervention (16 days)
COMPLETED
27
21

Reasons for withdrawal

Reasons for withdrawal
Measure
Morning Sodium Intervention, Then Evening Sodium Intervention
The study was conducted as a randomized, crossover feeding trial. During the first seven days of this period (run-in period), participants consumed only the study meals to standardize sodium intake. During the final nine days of this period (intervention period), participants continued to eat study meals and additionally consumed a chicken-based broth containing a total of 2 grams of sodium with breakfast (morning sodium). After a 4-week washout period, participants crossed over to the second study arm in which they consumed the study meals for seven days, and then consumed the study meals + a chicken-based broth containing a total of 2 grams of sodium with dinner for nine days (evening sodium).
Evening Sodium Intervention, Followed by Morning Sodium Intervention
The study was conducted as a randomized, crossover feeding trial. During the first seven days of this period (run-in period), participants consumed only the study meals to standardize sodium intake. During the final nine days of this period (intervention period), participants continued to eat study meals and additionally consumed a chicken-based broth containing a total of 2 grams of sodium with dinner (evening sodium). After a 4-week washout period, participants crossed over to the second study arm in which they consumed the study meals for seven days, and then consumed the study meals + a chicken-based broth containing a total of 2 grams of sodium with breakfast for nine days (morning sodium).
First Intervention (16 days)
Withdrawal by Subject
5
3
Washout (4 weeks)
Withdrawal by Subject
3
2

Baseline Characteristics

Timing of Sodium Intake and Nocturnal Sodium Excretion and Blood Pressure in Obese African Americans

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
Morning Sodium Intervention, Then Evening Sodium Intervention
n=35 Participants
Participants consumed only the study meals during the first seven days of the intervention, followed by eating study meals and a chicken-based broth containing a total of 2 grams of sodium with breakfast for the final nine days of the first intervention period (morning sodium). After a 4-week washout period, participants crossed over to the second intervention period in which they consumed the study meals for seven days, and then consumed the study meals plus broth containing a total of 2 grams of sodium with dinner for nine days (evening sodium).
Evening Sodium Intervention, Then Morning Sodium Intervention
n=26 Participants
Participants consumed only the study meals during the first seven days of the intervention, followed by eating study meals and a chicken-based broth containing a total of 2 grams of sodium with dinner for the final nine days of the first intervention period (evening sodium). After a 4-week washout period, participants crossed over to the second intervention period in which they consumed the study meals for seven days, and then consumed the study meals plus broth containing a total of 2 grams of sodium with breakfast for nine days (morning sodium).
Total
n=61 Participants
Total of all reporting groups
Age, Continuous
36 Years
STANDARD_DEVIATION 6 • n=5 Participants
37 Years
STANDARD_DEVIATION 5 • n=109 Participants
36 Years
STANDARD_DEVIATION 6 • n=133 Participants
Sex: Female, Male
Female
26 Participants
n=5 Participants
20 Participants
n=109 Participants
46 Participants
n=133 Participants
Sex: Female, Male
Male
9 Participants
n=5 Participants
6 Participants
n=109 Participants
15 Participants
n=133 Participants
Race (NIH/OMB)
American Indian or Alaska Native
0 Participants
n=5 Participants
0 Participants
n=109 Participants
0 Participants
n=133 Participants
Race (NIH/OMB)
Asian
0 Participants
n=5 Participants
0 Participants
n=109 Participants
0 Participants
n=133 Participants
Race (NIH/OMB)
Native Hawaiian or Other Pacific Islander
0 Participants
n=5 Participants
0 Participants
n=109 Participants
0 Participants
n=133 Participants
Race (NIH/OMB)
Black or African American
23 Participants
n=5 Participants
19 Participants
n=109 Participants
42 Participants
n=133 Participants
Race (NIH/OMB)
White
12 Participants
n=5 Participants
7 Participants
n=109 Participants
19 Participants
n=133 Participants
Race (NIH/OMB)
More than one race
0 Participants
n=5 Participants
0 Participants
n=109 Participants
0 Participants
n=133 Participants
Race (NIH/OMB)
Unknown or Not Reported
0 Participants
n=5 Participants
0 Participants
n=109 Participants
0 Participants
n=133 Participants

PRIMARY outcome

Timeframe: On the 8th day of consuming the standarized diet plus chicken-based broth

Population: A total of 8 participants withdrew from the study before primary outcome measures were able to be collected

Systolic blood pressure measured during sleep by 24-hour ambulatory blood pressure monitor starting on the 8th day of consuming the standarized diet plus chicken-based broth

Outcome measures

Outcome measures
Measure
Morning Sodium
n=53 Participants
Participants consumed a standardized diet providing 2.3 g of sodium per day for 7 days (run-in period), after which they consumed the same standardized diet for 9 days and in addition received 2 g of sodium in the form of a chicken-based broth with breakfast each day.
Evening Sodium
n=53 Participants
Participants consumed a standardized diet providing 2.3 g of sodium per day for 7 days (run-in period), after which they consumed the same standardized diet for 9 days and in addition received 2 g of sodium in the form of a chicken-based broth with dinner each day.
Sleep Systolic Blood Pressure
122.7 mmHg
Interval 117.3 to 128.1
118.9 mmHg
Interval 113.4 to 124.5

SECONDARY outcome

Timeframe: On the 9th day of consuming the standarized diet plus chicken-based broth

Population: A total of 8 participants withdrew from the study before outcome measures could be collected

Urinary sodium excretion measured in study arms by 24-hour urine collection starting on the 9th day of consuming the standarized diet plus chicken-based broth

Outcome measures

Outcome measures
Measure
Morning Sodium
n=53 Participants
Participants consumed a standardized diet providing 2.3 g of sodium per day for 7 days (run-in period), after which they consumed the same standardized diet for 9 days and in addition received 2 g of sodium in the form of a chicken-based broth with breakfast each day.
Evening Sodium
n=53 Participants
Participants consumed a standardized diet providing 2.3 g of sodium per day for 7 days (run-in period), after which they consumed the same standardized diet for 9 days and in addition received 2 g of sodium in the form of a chicken-based broth with dinner each day.
24-hour Urinary Sodium Excretion
213.2 mmoL
Standard Deviation 76.1
199.3 mmoL
Standard Deviation 76

SECONDARY outcome

Timeframe: On the 9th day of consuming the standarized diet plus chicken-based broth

Population: A total of 8 participants withdrew from the study before primary outcome measures were able to be collected

Plasma melatonin concentrations measured in blood samples collected every 2 hours for 24 hours starting on the 9th day of consuming the standarized diet plus chicken-based broth

Outcome measures

Outcome measures
Measure
Morning Sodium
n=53 Participants
Participants consumed a standardized diet providing 2.3 g of sodium per day for 7 days (run-in period), after which they consumed the same standardized diet for 9 days and in addition received 2 g of sodium in the form of a chicken-based broth with breakfast each day.
Evening Sodium
n=53 Participants
Participants consumed a standardized diet providing 2.3 g of sodium per day for 7 days (run-in period), after which they consumed the same standardized diet for 9 days and in addition received 2 g of sodium in the form of a chicken-based broth with dinner each day.
Concentrations of Plasma Melatonin
5 pg/ml
Interval 2.0 to 16.0
5 pg/ml
Interval 2.0 to 17.0

SECONDARY outcome

Timeframe: On the 9th day of consuming the standarized diet plus chicken-based broth

Population: A total of 8 participants withdrew from the study before primary outcome measures were able to be collected

Plasma cortisol concentrations measured in study participants every 2 hours for 24 hours starting on the 9th day of consuming the standarized diet plus chicken-based broth

Outcome measures

Outcome measures
Measure
Morning Sodium
n=53 Participants
Participants consumed a standardized diet providing 2.3 g of sodium per day for 7 days (run-in period), after which they consumed the same standardized diet for 9 days and in addition received 2 g of sodium in the form of a chicken-based broth with breakfast each day.
Evening Sodium
n=53 Participants
Participants consumed a standardized diet providing 2.3 g of sodium per day for 7 days (run-in period), after which they consumed the same standardized diet for 9 days and in addition received 2 g of sodium in the form of a chicken-based broth with dinner each day.
Concentrations of Plasma Cortisol
3.2 ug/dL
Interval 2.6 to 4.1
3.6 ug/dL
Interval 2.8 to 4.4

SECONDARY outcome

Timeframe: On the 9th day of consuming the standarized diet plus chicken-based broth

Population: A total of 8 participants withdrew from the study before primary outcome measures were able to be collected

Plasma endothelin 1 concentrations measured every 2 hours for 24 hours starting on the 9th day of consuming the standarized diet plus chicken-based broth

Outcome measures

Outcome measures
Measure
Morning Sodium
n=53 Participants
Participants consumed a standardized diet providing 2.3 g of sodium per day for 7 days (run-in period), after which they consumed the same standardized diet for 9 days and in addition received 2 g of sodium in the form of a chicken-based broth with breakfast each day.
Evening Sodium
n=53 Participants
Participants consumed a standardized diet providing 2.3 g of sodium per day for 7 days (run-in period), after which they consumed the same standardized diet for 9 days and in addition received 2 g of sodium in the form of a chicken-based broth with dinner each day.
Concentrations of Plasma Endothelin 1
1.4 pg/ml
Interval 0.6 to 1.8
1.2 pg/ml
Interval 0.8 to 1.8

SECONDARY outcome

Timeframe: On the 9th day of consuming the standarized diet plus chicken-based broth

Population: A total of 8 participants withdrew from the study prior to collection of study outcome data.

Plasma aldosterone concentrations measured every 2 hours for 24 hours starting on the 9th day of consuming the standarized diet plus chicken-based broth

Outcome measures

Outcome measures
Measure
Morning Sodium
n=53 Participants
Participants consumed a standardized diet providing 2.3 g of sodium per day for 7 days (run-in period), after which they consumed the same standardized diet for 9 days and in addition received 2 g of sodium in the form of a chicken-based broth with breakfast each day.
Evening Sodium
n=53 Participants
Participants consumed a standardized diet providing 2.3 g of sodium per day for 7 days (run-in period), after which they consumed the same standardized diet for 9 days and in addition received 2 g of sodium in the form of a chicken-based broth with dinner each day.
Concentrations of Plasma Aldosterone
101 ng/dL
Interval 62.0 to 201.0
103 ng/dL
Interval 66.0 to 223.0

SECONDARY outcome

Timeframe: On the 8th day of consuming the standardized diet plus chicken-based broth

Core Body Temperature measured using BodyCap On the 8th day of consuming the standardized diet plus chicken-based broth

Outcome measures

Outcome data not reported

SECONDARY outcome

Timeframe: On the 9th day of consuming the standardized diet plus chicken-based broth

Timing of melatonin increase based on every 2-hour blood draws during the 24 hour admission on the 9th day of consuming the standardized diet plus chicken-based broth

Outcome measures

Outcome data not reported

SECONDARY outcome

Timeframe: On the 9th day of consuming the standarized diet plus chicken-based broth

Timing of clock gene expression change based on every 2-hour blood draws during the 24 hour admission on the 9th day of consuming the standardized diet plus chicken-based broth

Outcome measures

Outcome data not reported

SECONDARY outcome

Timeframe: On the 9th day of consuming the standarized diet plus chicken-based broth

Changes in plasma cytokines based on every 2-hour blood draws during the 24 hour admission on the 9th day of consuming the standardized diet plus chicken-based broth

Outcome measures

Outcome data not reported

SECONDARY outcome

Timeframe: On the 9th day of consuming the standarized diet plus chicken-based broth

Change in circulating immune cell proportions based on every 2-hour blood draws during the 24 hour admission on the 9th day of consuming the standardized diet plus chicken-based broth

Outcome measures

Outcome data not reported

Adverse Events

Morning Sodium

Serious events: 0 serious events
Other events: 0 other events
Deaths: 0 deaths

Evening Sodium

Serious events: 0 serious events
Other events: 0 other events
Deaths: 0 deaths

Serious adverse events

Adverse event data not reported

Other adverse events

Adverse event data not reported

Additional Information

Orlando Gutierrez

University of Alabama at Birmingham

Phone: 205-975-2147

Results disclosure agreements

  • Principal investigator is a sponsor employee
  • Publication restrictions are in place