Detection of Bloodstream Pathogens in Hematological Malignancies

Summary

* To identify the common bacterial and fungal species causing fungemia and bacteremia in hematological malignancies.
* To identify sensitivity pattern for causative microbes.
* Compare culture on ordinary media with Vitek2 (automated microbial identification system) and multiplex polymerase chain reaction (PCR )

Conditions

• Bloodstream Infection

Interventions

DIAGNOSTIC_TEST

blood culture

During the febrile episode, two blood samples per patient will draw from two separate sites including central venous catheter if present and directly injected to Bact/Alert® bottles and incubated in Bact/ALERT system instrument (bioMérieux Diagnostics, Lyon, France). A-Identification of microorganisms including: 1. Isolation of microorganisms: 2. Blood culture samples with positive signals will be cultured on blood agar, chocolate agar , MacConkeys agar and Sabouraud dextrose agar (SDA) plates. B-Identification of the bacterial organism Pure colonies of isolated microorganisms were identified by: Morphology on agar,Gram stain film was made from the growth to identify morphology of the organism ,Biochemical tests C-For fungi isolate the following will perform: 1. Microscopic examination of Lactophenol cotton blue (LCB) wet mounts. 2. Culture on Brilliance Candida differential agar

OTHER

Anti fungal susceptibility testing or antibiotic susceptibility testing

isolation of microbes from positive blood culture and anti fungal susceptibility testing or antibiotic susceptibility testing according to isolated microbes by disc diffusion method and Vitek2

OTHER

phenotypic identification methods of the isolated organism by Vitek2 (BioMerieux,France)

The VITEK 2 is an automated microbial identification system that utilizing growth-based technology. With its colorimetric reagent cards, the VITEK 2 offers a state of the art technology platform for phenotypic identification methods.

OTHER

Identification of the isolated organism by multiplex PCR

FilmArray blood culture identification (BCID) is automated multiplex PCR assay, the FilmArray blood culture identification which directly identifies common pathogens, including 7 genera/ species of Gram-positive bacteria, 10 genera/species of Gram-negative bacteria, and 5 species of Candida (as well as 3 resistance determinants) in the positive blood culture bottles. The assay requires about 2 min of hands-on sample processing time and 1 h of instrument time (which includes DNA isolation, amplification, and detection).

Sponsors & Collaborators

• Assiut University

  lead OTHER

Principal Investigators

• Mohamed Z Abo krisha, professor · Assiut University

Eligibility

Min Age

18 Years

Sex

ALL

Healthy Volunteers

No

Timeline & Regulatory

Start

2020-01-01

Primary Completion

2022-02-28

Completion

2022-05-01

Countries

• Egypt

Study Locations