Trial Outcomes & Findings for A Methodology Development Clinical Trial of Reproxalap in Subjects With Seasonal Allergic Conjunctivitis Using the Environmental Exposure Chamber (NCT NCT03709121)

NCT ID: NCT03709121

Last Updated: 2025-02-24

Results Overview

Change from baseline comparison of reproxalap to vehicle for subject-reported ocular itching score from 0 to 212 minutes in the allergen chamber using a 0 -100 millimeter visual analogue scale (0 = none, 100 = severe) was assessed. The least squares mean (standard error) was derived from mixed model repeated measures for change from baseline included itching score as a dependent variable, treatment, period, and interaction of treatment and period as fixed effects.

Recruitment status

COMPLETED

Study phase

PHASE1/PHASE2

Target enrollment

70 participants

Primary outcome timeframe

Efficacy was assessed from 0 to 212 minutes in the allergen chamber.

Results posted on

2025-02-24

Participant Flow

Seventy subjects were randomized in a three-way crossover design trial.

Participant milestones

Participant milestones
Measure
Reproxalap (0.25%), Then Reproxalap (0.5%), Then Vehicle
Reproxalap (0.25%) was administered once 5 minutes before and 90 minutes after allergen chamber entry, then there was a 14-day washout (Washout #1), then Reproxalap (0.5%) was administered once 5 minutes before and 90 minutes after allergen chamber entry, then there was a 14-day (Washout #2), then Vehicle was administered once 5 minutes before and 90 minutes after allergen chamber entry.
Reproxalap (0.5%), Then Vehicle, Then Reproxalap (0.25%)
Reproxalap (0.5%) was administered once 5 minutes before and 90 minutes after allergen chamber entry, then there was a 14-day washout (Washout #1), then Vehicle was administered once 5 minutes before and 90 minutes after allergen chamber entry, then there was a 14-day (Washout #2), then Reproxalap (0.25%) was administered once 5 minutes before and 90 minutes after allergen chamber entry.
Vehicle, Then Reproxalap (0.25%), Then Reproxalap (0.5%)
Vehicle was administered once 5 minutes before and 90 minutes after allergen chamber entry, then there was a 14-day washout (Washout #1), then Reproxalap (0.25%) was administered once 5 minutes before and 90 minutes after allergen chamber entry, then there was a 14-day (Washout #2), then Reproxalap (0.5%) was administered once 5 minutes before and 90 minutes after allergen chamber entry.
Overall Study
STARTED
22
25
23
Overall Study
COMPLETED
22
22
22
Overall Study
NOT COMPLETED
0
3
1

Reasons for withdrawal

Withdrawal data not reported

Baseline Characteristics

A Methodology Development Clinical Trial of Reproxalap in Subjects With Seasonal Allergic Conjunctivitis Using the Environmental Exposure Chamber

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
Reproxalap (0.25%), Then Reproxalap (0.5%), Then Vehicle
n=22 Participants
Reproxalap (0.25%) was administered once 5 minutes before and 90 minutes after allergen chamber entry, then there was a 14-day washout (Washout #1), then Reproxalap (0.5%) was administered once 5 minutes before and 90 minutes after allergen chamber entry, then there was a 14-day (Washout #2), then Vehicle was administered once 5 minutes before and 90 minutes after allergen chamber entry.
Reproxalap (0.5%), Then Vehicle, Then Reproxalap (0.25%)
n=25 Participants
Reproxalap (0.5%) was administered once 5 minutes before and 90 minutes after allergen chamber entry, then there was a 14-day washout (Washout #1), then Vehicle) was administered once 5 minutes before and 90 minutes after allergen chamber entry, then there was a 14-day (Washout #2), then Reproxalap (0.25%) was administered once 5 minutes before and 90 minutes after allergen chamber entry.
Vehicle, Then Reproxalap (0.25%), Then Reproxalap (0.5%)
n=23 Participants
Vehicle was administered once 5 minutes before and 90 minutes after allergen chamber entry, then there was a 14-day washout (Washout #1), then Reproxalap (0.25%) was administered once 5 minutes before and 90 minutes after allergen chamber entry, then there was a 14-day (Washout #2), then Reproxalap (0.5%) was administered once 5 minutes before and 90 minutes after allergen chamber entry.
Total
n=70 Participants
Total of all reporting groups
Age, Customized
Mean
45.50 years
n=99 Participants
48.84 years
n=107 Participants
46.17 years
n=206 Participants
46.9 years
n=7 Participants
Sex: Female, Male
Female
12 Participants
n=99 Participants
16 Participants
n=107 Participants
14 Participants
n=206 Participants
42 Participants
n=7 Participants
Sex: Female, Male
Male
10 Participants
n=99 Participants
9 Participants
n=107 Participants
9 Participants
n=206 Participants
28 Participants
n=7 Participants
Ethnicity (NIH/OMB)
Hispanic or Latino
2 Participants
n=99 Participants
2 Participants
n=107 Participants
5 Participants
n=206 Participants
9 Participants
n=7 Participants
Ethnicity (NIH/OMB)
Not Hispanic or Latino
20 Participants
n=99 Participants
23 Participants
n=107 Participants
18 Participants
n=206 Participants
61 Participants
n=7 Participants
Ethnicity (NIH/OMB)
Unknown or Not Reported
0 Participants
n=99 Participants
0 Participants
n=107 Participants
0 Participants
n=206 Participants
0 Participants
n=7 Participants
Race/Ethnicity, Customized
White
15 Participants
n=99 Participants
17 Participants
n=107 Participants
13 Participants
n=206 Participants
45 Participants
n=7 Participants
Race/Ethnicity, Customized
American Indian or Alaska Native
0 Participants
n=99 Participants
0 Participants
n=107 Participants
0 Participants
n=206 Participants
0 Participants
n=7 Participants
Race/Ethnicity, Customized
Black or African American
4 Participants
n=99 Participants
5 Participants
n=107 Participants
5 Participants
n=206 Participants
14 Participants
n=7 Participants
Race/Ethnicity, Customized
Native Hawaiian / Other Pacific Islander
0 Participants
n=99 Participants
0 Participants
n=107 Participants
0 Participants
n=206 Participants
0 Participants
n=7 Participants
Race/Ethnicity, Customized
Asian
1 Participants
n=99 Participants
3 Participants
n=107 Participants
5 Participants
n=206 Participants
9 Participants
n=7 Participants
Race/Ethnicity, Customized
Other - Mixed
2 Participants
n=99 Participants
0 Participants
n=107 Participants
0 Participants
n=206 Participants
2 Participants
n=7 Participants
Region of Enrollment
Canada
22 participants
n=99 Participants
25 participants
n=107 Participants
23 participants
n=206 Participants
70 participants
n=7 Participants

PRIMARY outcome

Timeframe: Efficacy was assessed from 0 to 212 minutes in the allergen chamber.

Population: Intent-to-treat population

Change from baseline comparison of reproxalap to vehicle for subject-reported ocular itching score from 0 to 212 minutes in the allergen chamber using a 0 -100 millimeter visual analogue scale (0 = none, 100 = severe) was assessed. The least squares mean (standard error) was derived from mixed model repeated measures for change from baseline included itching score as a dependent variable, treatment, period, and interaction of treatment and period as fixed effects.

Outcome measures

Outcome measures
Measure
Reproxalap (0.25%)
n=67 Participants
Reproxalap was administered once 5 minutes before and 90 minutes after allergen chamber entry.
Reproxalap (0.5%)
n=69 Participants
Reproxalap was administered once 5 minutes before and 90 minutes after allergen chamber entry.
Vehicle
n=67 Participants
Vehicle was administered once 5 minutes before and 90 minutes after allergen chamber entry.
Subject-Reported Ocular Itching Score
16.410 units on a scale
Standard Error 1.6195
16.964 units on a scale
Standard Error 1.6150
22.310 units on a scale
Standard Error 1.6188

SECONDARY outcome

Timeframe: Efficacy was assessed from 0 to 212 minutes in the allergen chamber.

Population: Intent-to-treat population

Change from baseline comparison of reproxalap to vehicle for subject-reported ocular tearing score from 0 to 212 minutes in the allergen chamber using a 4-point scale (0 = none, 3 = severe) was assessed. The least squares mean (standard error) was derived from mixed model repeated measures for change from baseline included itching score as a dependent variable, treatment, period, and interaction of treatment and period as fixed effects.

Outcome measures

Outcome measures
Measure
Reproxalap (0.25%)
n=67 Participants
Reproxalap was administered once 5 minutes before and 90 minutes after allergen chamber entry.
Reproxalap (0.5%)
n=69 Participants
Reproxalap was administered once 5 minutes before and 90 minutes after allergen chamber entry.
Vehicle
n=67 Participants
Vehicle was administered once 5 minutes before and 90 minutes after allergen chamber entry.
Subject-Reported Ocular Tearing Score
0.8777 units on a scale
Standard Error 0.0615
0.8915 units on a scale
Standard Error 0.0613
1.0507 units on a scale
Standard Error 0.0614

SECONDARY outcome

Timeframe: Efficacy was assessed from 0 to 210 minutes in the allergen chamber.

Population: Intent-to-treat population

Change from baseline comparison of reproxalap to vehicle for investigator-assessed conjunctival redness from 0 to 210 minutes in the allergen chamber using a 9-point scale with half unit increments (0 = normal, 4 = prominent) was assessed. The least squares mean (standard error) was derived from mixed model repeated measures for change from baseline included itching score as a dependent variable, treatment, period, and interaction of treatment and period as fixed effects.

Outcome measures

Outcome measures
Measure
Reproxalap (0.25%)
n=67 Participants
Reproxalap was administered once 5 minutes before and 90 minutes after allergen chamber entry.
Reproxalap (0.5%)
n=69 Participants
Reproxalap was administered once 5 minutes before and 90 minutes after allergen chamber entry.
Vehicle
n=67 Participants
Vehicle was administered once 5 minutes before and 90 minutes after allergen chamber entry.
Investigator-Assessed Conjunctival Redness Score
0.5004 units on a scale
Standard Error 0.0458
0.5462 units on a scale
Standard Error 0.0457
0.6497 units on a scale
Standard Error 0.0458

Adverse Events

Reproxalap (0.25%)

Serious events: 0 serious events
Other events: 49 other events
Deaths: 0 deaths

Reproxalap (0.5%)

Serious events: 0 serious events
Other events: 56 other events
Deaths: 0 deaths

Vehicle Ophthalmic Solution

Serious events: 0 serious events
Other events: 5 other events
Deaths: 0 deaths

Serious adverse events

Adverse event data not reported

Other adverse events

Other adverse events
Measure
Reproxalap (0.25%)
n=67 participants at risk
Reproxalap was administered once 5 minutes before and 90 minutes after allergen chamber entry.
Reproxalap (0.5%)
n=69 participants at risk
Reproxalap was administered once 5 minutes before and 90 minutes after allergen chamber entry.
Vehicle Ophthalmic Solution
n=67 participants at risk
Vehicle was administered once 5 minutes before and 90 minutes after allergen chamber entry.
General disorders
General disorders and administration site conditions
73.1%
49/67 • The period of time over which adverse events were collected for each subject in the clinical trial was approximately fourteen weeks.
81.2%
56/69 • The period of time over which adverse events were collected for each subject in the clinical trial was approximately fourteen weeks.
7.5%
5/67 • The period of time over which adverse events were collected for each subject in the clinical trial was approximately fourteen weeks.

Additional Information

VP, Clinical Operations

Aldeyra Therapeutics, Inc.

Phone: 781-257-3063

Results disclosure agreements

  • Principal investigator is a sponsor employee
  • Publication restrictions are in place