Trichomonas Vaginalis Genotyping in Upper Egypt
NCT03614286 · Status: UNKNOWN · Type: OBSERVATIONAL · Enrollment: 20
Last updated 2018-08-07
Summary
The worldwide incidence of trichomoniasis was estimated to be 276.4 million new cases per year in 2008 .In Egypt, the reported prevalence rate ranges from 5% to 79.16% we aim to study genetic variability of Trichomonas vaginalis using PCR
Conditions
- Trichomonas Vaginalis Genotyping in Upper Egypt
- Vaginitis Trichomonal
Interventions
- DIAGNOSTIC_TEST
-
culture and pcr
The swab immersed in Diamond's Modified medium culture tube and squeezed for cultivation and examined daily with a light microscope to identify T. vaginalis Samples from culture will be placed in 1 ml of a commercial PCR transport medium (AMPLICOR; Roche Diagnostic Systems, Branchburg, N.J.) and kept at 4°C until arrival at the laboratory within 4 days of collection. An equal volume of specimen diluent (AMPLICOR) was added to the sample , and the preparation was mixed, incubated at room temperature for 10 min, and stored at -70°C until tested.
Sponsors & Collaborators
-
Assiut University
lead OTHER
Eligibility
- Sex
- FEMALE
- Healthy Volunteers
- No
Timeline & Regulatory
- Start
- 2018-10-31
- Primary Completion
- 2019-10-31
- Completion
- 2020-03-31
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