Trial Outcomes & Findings for Ivermectin and Human Immunity (NCT NCT03459794)

NCT ID: NCT03459794

Last Updated: 2019-07-12

Results Overview

Changes in serum levels of a panel of 41 cytokines will be compared to baseline levels using Luminex methods (HCYTOMAG-60K-PX41 kit from EMD Millipore). No pre-specified threshold was set for biological significance, and the number of cytokines showing a statistically significant (p=\<0.05) change from time 0 for each group will be reported. The number of cytokines with significant changes is taken from a comparison of the mean levels in each of the groups, not at the level of individual participants.

Recruitment status

COMPLETED

Study phase

EARLY_PHASE1

Target enrollment

12 participants

Primary outcome timeframe

Pre-treatment, 4 hours and 24 hours post-treatment

Results posted on

2019-07-12

Participant Flow

Participant milestones

Participant milestones
Measure
Ivermectin
Ivermectin will be administered once at 150mcg/kg, orally. Ivermectin: 150 mcg/kg ivermectin, by mouth.
Control
An oral placebo will be administered once Placebo: An oral placebo will be administered, once
Overall Study
STARTED
8
4
Overall Study
COMPLETED
8
4
Overall Study
NOT COMPLETED
0
0

Reasons for withdrawal

Withdrawal data not reported

Baseline Characteristics

Ivermectin and Human Immunity

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
Ivermectin
n=8 Participants
Ivermectin will be administered once at 150mcg/kg, orally. Ivermectin: 150 mcg/kg ivermectin, by mouth.
Control
n=4 Participants
An oral placebo will be administered once Placebo: An oral placebo will be administered, once
Total
n=12 Participants
Total of all reporting groups
Race (NIH/OMB)
Unknown or Not Reported
1 Participants
n=39 Participants
0 Participants
n=41 Participants
1 Participants
n=35 Participants
Region of Enrollment
United States
8 participants
n=39 Participants
4 participants
n=41 Participants
12 participants
n=35 Participants
Age, Categorical
<=18 years
0 Participants
n=39 Participants
0 Participants
n=41 Participants
0 Participants
n=35 Participants
Age, Categorical
Between 18 and 65 years
8 Participants
n=39 Participants
4 Participants
n=41 Participants
12 Participants
n=35 Participants
Age, Categorical
>=65 years
0 Participants
n=39 Participants
0 Participants
n=41 Participants
0 Participants
n=35 Participants
Sex: Female, Male
Female
5 Participants
n=39 Participants
4 Participants
n=41 Participants
9 Participants
n=35 Participants
Sex: Female, Male
Male
3 Participants
n=39 Participants
0 Participants
n=41 Participants
3 Participants
n=35 Participants
Ethnicity (NIH/OMB)
Hispanic or Latino
1 Participants
n=39 Participants
1 Participants
n=41 Participants
2 Participants
n=35 Participants
Ethnicity (NIH/OMB)
Not Hispanic or Latino
6 Participants
n=39 Participants
3 Participants
n=41 Participants
9 Participants
n=35 Participants
Ethnicity (NIH/OMB)
Unknown or Not Reported
1 Participants
n=39 Participants
0 Participants
n=41 Participants
1 Participants
n=35 Participants
Race (NIH/OMB)
American Indian or Alaska Native
0 Participants
n=39 Participants
0 Participants
n=41 Participants
0 Participants
n=35 Participants
Race (NIH/OMB)
Asian
0 Participants
n=39 Participants
0 Participants
n=41 Participants
0 Participants
n=35 Participants
Race (NIH/OMB)
Native Hawaiian or Other Pacific Islander
0 Participants
n=39 Participants
0 Participants
n=41 Participants
0 Participants
n=35 Participants
Race (NIH/OMB)
Black or African American
0 Participants
n=39 Participants
0 Participants
n=41 Participants
0 Participants
n=35 Participants
Race (NIH/OMB)
White
7 Participants
n=39 Participants
4 Participants
n=41 Participants
11 Participants
n=35 Participants
Race (NIH/OMB)
More than one race
0 Participants
n=39 Participants
0 Participants
n=41 Participants
0 Participants
n=35 Participants

PRIMARY outcome

Timeframe: Pre-treatment, 4 hours and 24 hours post-treatment

Population: Cytokines were measured in sera using the HCYTOMAG-60K-PX41 kit from EMD Millipore. The numbers reported are the number of cytokines with statistically significant changes ( p = \<0.05) from pre-treatment levels.

Changes in serum levels of a panel of 41 cytokines will be compared to baseline levels using Luminex methods (HCYTOMAG-60K-PX41 kit from EMD Millipore). No pre-specified threshold was set for biological significance, and the number of cytokines showing a statistically significant (p=\<0.05) change from time 0 for each group will be reported. The number of cytokines with significant changes is taken from a comparison of the mean levels in each of the groups, not at the level of individual participants.

Outcome measures

Outcome measures
Measure
Ivermectin 4hrs
n=41 Cytokines
Ivermectin will be administered once at 150mcg/kg, orally. Ivermectin: 150 mcg/kg ivermectin, by mouth. 4 hrs post-treatment
Ivermectin 24 Hrs
n=41 Cytokines
Ivermectin will be administered once at 150mcg/kg, orally. Ivermectin: 150 mcg/kg ivermectin, by mouth.24 hrs post-treatment
Control 4 Hrs
n=41 Cytokines
An oral placebo will be administered once Placebo: An oral placebo will be administered, once. Sera collected 4hrs post-treatment
Control 24 Hrs
n=41 Cytokines
An oral placebo will be administered once Placebo: An oral placebo will be administered, once. Sera collected 24hrs post-treatment
The Number of Cytokines Showing Statistically Significant Changes From Pre-treatment Levels Will be Recorded.
0 Cytokines changed from t=0
0 Cytokines changed from t=0
0 Cytokines changed from t=0
0 Cytokines changed from t=0

PRIMARY outcome

Timeframe: Pre-treatment, 4 hours and 24 hours post-treatment

Population: Levels of 770 messenger RNAs (mRNAs) were assayed in PBMC isolated from study participants using the Human Nanostring Myeloid cell panel.

Changes in expression levels of approximately 770 genes involved in innate immunity will be measured in peripheral blood mononuclear cells (PBMC) before and after treatment. The number of transcripts with significant changes is taken from a comparison of the mean levels in each of the groups, not at the individual participant level. No pre-determined threshold was set for the biological significance of these changes.

Outcome measures

Outcome measures
Measure
Ivermectin 4hrs
n=8 Participants
Ivermectin will be administered once at 150mcg/kg, orally. Ivermectin: 150 mcg/kg ivermectin, by mouth. 4 hrs post-treatment
Ivermectin 24 Hrs
n=8 Participants
Ivermectin will be administered once at 150mcg/kg, orally. Ivermectin: 150 mcg/kg ivermectin, by mouth.24 hrs post-treatment
Control 4 Hrs
n=4 Participants
An oral placebo will be administered once Placebo: An oral placebo will be administered, once. Sera collected 4hrs post-treatment
Control 24 Hrs
n=4 Participants
An oral placebo will be administered once Placebo: An oral placebo will be administered, once. Sera collected 24hrs post-treatment
Number of Transcripts in PBMC With Statistically Significant Changes From Pre-treatment Levels.
0 Transcripts signicantly changed from t=0
0 Transcripts signicantly changed from t=0
10 Transcripts signicantly changed from t=0
0 Transcripts signicantly changed from t=0

SECONDARY outcome

Timeframe: Pre-treatment (0hrs), 24 hours

CBCs will be performed before treatment and 24 hrs later

Outcome measures

Outcome measures
Measure
Ivermectin 4hrs
n=8 Participants
Ivermectin will be administered once at 150mcg/kg, orally. Ivermectin: 150 mcg/kg ivermectin, by mouth. 4 hrs post-treatment
Ivermectin 24 Hrs
n=8 Participants
Ivermectin will be administered once at 150mcg/kg, orally. Ivermectin: 150 mcg/kg ivermectin, by mouth.24 hrs post-treatment
Control 4 Hrs
n=4 Participants
An oral placebo will be administered once Placebo: An oral placebo will be administered, once. Sera collected 4hrs post-treatment
Control 24 Hrs
n=4 Participants
An oral placebo will be administered once Placebo: An oral placebo will be administered, once. Sera collected 24hrs post-treatment
Complete Blood Counts (CBC)
Neutrophils
3.069 Million cells/mL
Standard Error 0.353
3.341 Million cells/mL
Standard Error 0.426
2.940 Million cells/mL
Standard Error 0.18
3.358 Million cells/mL
Standard Error 0.47
Complete Blood Counts (CBC)
Lymphocytes
1.753 Million cells/mL
Standard Error 0.22
1.864 Million cells/mL
Standard Error 0.151
1.833 Million cells/mL
Standard Error 0.161
2.075 Million cells/mL
Standard Error 0.175
Complete Blood Counts (CBC)
Monocytes
0.483 Million cells/mL
Standard Error 0.045
0.403 Million cells/mL
Standard Error 0.057
0.373 Million cells/mL
Standard Error 0.04
0.375 Million cells/mL
Standard Error 0.034
Complete Blood Counts (CBC)
Eosinophils
0.208 Million cells/mL
Standard Error 0.055
0.205 Million cells/mL
Standard Error 0.047
0.158 Million cells/mL
Standard Error 0.015
0.170 Million cells/mL
Standard Error 0.018
Complete Blood Counts (CBC)
Basophils
0.04 Million cells/mL
Standard Error 0.008
0.046 Million cells/mL
Standard Error 0.006
0.048 Million cells/mL
Standard Error 0.008
0.048 Million cells/mL
Standard Error 0.0005

Adverse Events

Ivermectin

Serious events: 0 serious events
Other events: 1 other events
Deaths: 0 deaths

Control

Serious events: 0 serious events
Other events: 1 other events
Deaths: 0 deaths

Serious adverse events

Adverse event data not reported

Other adverse events

Other adverse events
Measure
Ivermectin
n=8 participants at risk
Ivermectin will be administered once at 150mcg/kg, orally. Ivermectin: 150 mcg/kg ivermectin, by mouth.
Control
n=4 participants at risk
An oral placebo will be administered once Placebo: An oral placebo will be administered, once
Gastrointestinal disorders
Minor gastrointestinal upset
12.5%
1/8 • 1 day following administration of the drug/placebo
25.0%
1/4 • 1 day following administration of the drug/placebo

Additional Information

Dr Adrian Wolstenholme

University of Georgia

Phone: 706 542 2404

Results disclosure agreements

  • Principal investigator is a sponsor employee
  • Publication restrictions are in place