Trial Outcomes & Findings for Impact of LDL-cholesterol Lowering on Platelet Activation (NCT NCT03331666)

NCT ID: NCT03331666

Last Updated: 2021-04-01

Results Overview

ADP- or arachidonic acid-stimulated platelet aggregation as assessed by the commercially-available VerifyNow P2Y12 (VerifyNow PRUTest) will be performed. Unit: PRU

Recruitment status

TERMINATED

Study phase

PHASE4

Target enrollment

4 participants

Primary outcome timeframe

Day 7, Day 14, Day 21, Day 28, Day 84

Results posted on

2021-04-01

Participant Flow

Participant milestones

Participant milestones
Measure
Evolocumab
Subjects will start with placebo and will receive Evolocumab 140 mg every 14 days starting Day 14 until Day 196. Evolocumab: 140 mg every 14 days A monoclonal antibody designed for the treatment of hyperlipidemia
Placebo
Subjects will start with placebo and will receive Evolocumab 140 mg every 14 days starting Day 28 until Day 196. Evolocumab: 140 mg every 14 days A monoclonal antibody designed for the treatment of hyperlipidemia
Overall Study
STARTED
2
2
Overall Study
COMPLETED
0
0
Overall Study
NOT COMPLETED
2
2

Reasons for withdrawal

Reasons for withdrawal
Measure
Evolocumab
Subjects will start with placebo and will receive Evolocumab 140 mg every 14 days starting Day 14 until Day 196. Evolocumab: 140 mg every 14 days A monoclonal antibody designed for the treatment of hyperlipidemia
Placebo
Subjects will start with placebo and will receive Evolocumab 140 mg every 14 days starting Day 28 until Day 196. Evolocumab: 140 mg every 14 days A monoclonal antibody designed for the treatment of hyperlipidemia
Overall Study
Due to Covid-19 pandemic, the trial was prematurely terminated
2
2

Baseline Characteristics

Impact of LDL-cholesterol Lowering on Platelet Activation

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
Evolocumab
n=2 Participants
Subjects will start with placebo and will receive Evolocumab 140 mg every 14 days starting Day 14 until Day 196. Evolocumab: 140 mg every 14 days A monoclonal antibody designed for the treatment of hyperlipidemia
Placebo
n=2 Participants
Subjects will start with placebo and will receive Evolocumab 140 mg every 14 days starting Day 28 until Day 196. Evolocumab: 140 mg every 14 days A monoclonal antibody designed for the treatment of hyperlipidemia
Total
n=4 Participants
Total of all reporting groups
Age, Categorical
<=18 years
0 Participants
n=39 Participants
0 Participants
n=41 Participants
0 Participants
n=35 Participants
Age, Categorical
Between 18 and 65 years
1 Participants
n=39 Participants
0 Participants
n=41 Participants
1 Participants
n=35 Participants
Age, Categorical
>=65 years
1 Participants
n=39 Participants
2 Participants
n=41 Participants
3 Participants
n=35 Participants
Sex: Female, Male
Female
1 Participants
n=39 Participants
1 Participants
n=41 Participants
2 Participants
n=35 Participants
Sex: Female, Male
Male
1 Participants
n=39 Participants
1 Participants
n=41 Participants
2 Participants
n=35 Participants
Ethnicity (NIH/OMB)
Hispanic or Latino
2 Participants
n=39 Participants
0 Participants
n=41 Participants
2 Participants
n=35 Participants
Ethnicity (NIH/OMB)
Not Hispanic or Latino
0 Participants
n=39 Participants
2 Participants
n=41 Participants
2 Participants
n=35 Participants
Ethnicity (NIH/OMB)
Unknown or Not Reported
0 Participants
n=39 Participants
0 Participants
n=41 Participants
0 Participants
n=35 Participants
Region of Enrollment
United States
2 participants
n=39 Participants
2 participants
n=41 Participants
4 participants
n=35 Participants

PRIMARY outcome

Timeframe: Day 7, Day 14, Day 21, Day 28, Day 84

Population: Adult male and female subjects who have a clinical diagnosis of familial hypercholesterolemia and referred for treatment with PCSK9 inhibitor, with LDL cholesterol levels ≥70 mg/dl on baseline treatment with statins and/or ezetimibe or intolerant to statin and/or ezetimibe. Of the four participants reported in this study, one participant per arm had ADP assessed by PRU Test (evolocumab and placebo arms) and one participant per arm had ADP assessed by Aspirin assay (evolocumab and placebo arms).

ADP- or arachidonic acid-stimulated platelet aggregation as assessed by the commercially-available VerifyNow P2Y12 (VerifyNow PRUTest) will be performed. Unit: PRU

Outcome measures

Outcome measures
Measure
Evolocumab
n=1 Participants
Subjects will start with placebo and will receive Evolocumab 140 mg every 14 days starting Day 14 until Day 196. Evolocumab: 140 mg every 14 days A monoclonal antibody designed for the treatment of hyperlipidemia
Placebo
n=1 Participants
Subjects will start with placebo and will receive Evolocumab 140 mg every 14 days starting Day 28 until Day 196. Evolocumab: 140 mg every 14 days A monoclonal antibody designed for the treatment of hyperlipidemia
Adenosine Di-phosphate (ADP) Induced, P2Y12 Dependent and Arachidonic Acid Induced Platelet Activation (P2Y12 Reaction Units (PRU))
P2Y12 Test, Day 7
266 PRU
216 PRU
Adenosine Di-phosphate (ADP) Induced, P2Y12 Dependent and Arachidonic Acid Induced Platelet Activation (P2Y12 Reaction Units (PRU))
P2Y12 Test, Day 14
268 PRU
226 PRU
Adenosine Di-phosphate (ADP) Induced, P2Y12 Dependent and Arachidonic Acid Induced Platelet Activation (P2Y12 Reaction Units (PRU))
P2Y12 test, Day 21
268 PRU
233 PRU
Adenosine Di-phosphate (ADP) Induced, P2Y12 Dependent and Arachidonic Acid Induced Platelet Activation (P2Y12 Reaction Units (PRU))
P2Y12 Test, Day 28
276 PRU
225 PRU
Adenosine Di-phosphate (ADP) Induced, P2Y12 Dependent and Arachidonic Acid Induced Platelet Activation (P2Y12 Reaction Units (PRU))
P2Y12 Test, Day 84
256 PRU
267 PRU

PRIMARY outcome

Timeframe: Day 7, Day 14, Day 21, Day 28, Day 84

Population: Adult male and female subjects who have a clinical diagnosis of familial hypercholesterolemia and referred for treatment with PCSK9 inhibitor, with LDL cholesterol levels ≥70 mg/dl on baseline treatment with statins and/or ezetimibe or intolerant to statin and/or ezetimibe. Of the four participants reported in this study, one participant per arm had ADP assessed by PRU Test (evolocumab and placebo arms) and one participant per arm had ADP assessed by Aspirin assay (evolocumab and placebo arms).

ADP- or arachidonic acid-stimulated platelet aggregation as assessed by the commercially-available VerifyNow Aspirin assay (Accriva Diagnostics) will be performed. Unit: Aspirin Reaction Unit (ARU)

Outcome measures

Outcome measures
Measure
Evolocumab
n=1 Participants
Subjects will start with placebo and will receive Evolocumab 140 mg every 14 days starting Day 14 until Day 196. Evolocumab: 140 mg every 14 days A monoclonal antibody designed for the treatment of hyperlipidemia
Placebo
n=1 Participants
Subjects will start with placebo and will receive Evolocumab 140 mg every 14 days starting Day 28 until Day 196. Evolocumab: 140 mg every 14 days A monoclonal antibody designed for the treatment of hyperlipidemia
Adenosine Di-phosphate (ADP) Induced, P2Y12 Dependent and Arachidonic Acid Induced Platelet Activation (Aspirin Reaction Unit (ARU))
Aspirin Test, Day 7
521 ARU
432 ARU
Adenosine Di-phosphate (ADP) Induced, P2Y12 Dependent and Arachidonic Acid Induced Platelet Activation (Aspirin Reaction Unit (ARU))
Aspirin Test Day 14
608 ARU
401 ARU
Adenosine Di-phosphate (ADP) Induced, P2Y12 Dependent and Arachidonic Acid Induced Platelet Activation (Aspirin Reaction Unit (ARU))
Aspirin Test, Day 21
533 ARU
490 ARU
Adenosine Di-phosphate (ADP) Induced, P2Y12 Dependent and Arachidonic Acid Induced Platelet Activation (Aspirin Reaction Unit (ARU))
Aspirin Test, Day 28
530 ARU
512 ARU
Adenosine Di-phosphate (ADP) Induced, P2Y12 Dependent and Arachidonic Acid Induced Platelet Activation (Aspirin Reaction Unit (ARU))
Aspirin Test, Day 84
406 ARU
386 ARU

Adverse Events

Evolocumab

Serious events: 0 serious events
Other events: 0 other events
Deaths: 0 deaths

Placebo

Serious events: 0 serious events
Other events: 0 other events
Deaths: 0 deaths

Serious adverse events

Adverse event data not reported

Other adverse events

Adverse event data not reported

Additional Information

Dr. Nan Wang

Columbia University Irving Medical Center

Phone: 212-342-1761

Results disclosure agreements

  • Principal investigator is a sponsor employee
  • Publication restrictions are in place