Trial Outcomes & Findings for HIV Reservoir Reduction With Interleukin-2 (NCT NCT03308786)

NCT ID: NCT03308786

Last Updated: 2022-09-28

Results Overview

Change in the number of infectious units per million resting CD4+ T cells (IUPM) from baseline to the end of study treatment, as measured by the Quantitative Viral Outgrowth Assay (QVOA).

Recruitment status

TERMINATED

Study phase

PHASE2

Target enrollment

9 participants

Primary outcome timeframe

15 months

Results posted on

2022-09-28

Participant Flow

Participant milestones

Participant milestones
Measure
IL2 Treatment
Subcutaneous recombinant interleukin-2 (rIL2), 5 MIU twice daily for four consecutive days(cycle) every 8 weeks for 8 cycles, in addition to combination antiretroviral therapy. Recombinant Interleukin-2: Subcutaneous recombinant interleukin-2 (rIL2), 5 MIU twice daily for four consecutive days(cycle) every 8 weeks for 8 cycles.
Overall Study
STARTED
9
Overall Study
COMPLETED
0
Overall Study
NOT COMPLETED
9

Reasons for withdrawal

Reasons for withdrawal
Measure
IL2 Treatment
Subcutaneous recombinant interleukin-2 (rIL2), 5 MIU twice daily for four consecutive days(cycle) every 8 weeks for 8 cycles, in addition to combination antiretroviral therapy. Recombinant Interleukin-2: Subcutaneous recombinant interleukin-2 (rIL2), 5 MIU twice daily for four consecutive days(cycle) every 8 weeks for 8 cycles.
Overall Study
study terminated after discussion with Safety Monitoring Committee
9

Baseline Characteristics

HIV Reservoir Reduction With Interleukin-2

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
IL2 Treatment
n=9 Participants
Subcutaneous recombinant interleukin-2 (rIL2), 5 MIU twice daily for four consecutive days(cycle) every 8 weeks for 8 cycles, in addition to combination antiretroviral therapy. Recombinant Interleukin-2: Subcutaneous recombinant interleukin-2 (rIL2), 5 MIU twice daily for four consecutive days(cycle) every 8 weeks for 8 cycles.
Age, Categorical
<=18 years
0 Participants
n=99 Participants
Age, Categorical
Between 18 and 65 years
9 Participants
n=99 Participants
Age, Categorical
>=65 years
0 Participants
n=99 Participants
Age, Continuous
49 years
n=99 Participants
Sex: Female, Male
Female
0 Participants
n=99 Participants
Sex: Female, Male
Male
9 Participants
n=99 Participants
Ethnicity (NIH/OMB)
Hispanic or Latino
1 Participants
n=99 Participants
Ethnicity (NIH/OMB)
Not Hispanic or Latino
8 Participants
n=99 Participants
Ethnicity (NIH/OMB)
Unknown or Not Reported
0 Participants
n=99 Participants
Race (NIH/OMB)
American Indian or Alaska Native
0 Participants
n=99 Participants
Race (NIH/OMB)
Asian
0 Participants
n=99 Participants
Race (NIH/OMB)
Native Hawaiian or Other Pacific Islander
0 Participants
n=99 Participants
Race (NIH/OMB)
Black or African American
2 Participants
n=99 Participants
Race (NIH/OMB)
White
6 Participants
n=99 Participants
Race (NIH/OMB)
More than one race
1 Participants
n=99 Participants
Race (NIH/OMB)
Unknown or Not Reported
0 Participants
n=99 Participants
Region of Enrollment
United States
9 participants
n=99 Participants
CD4+ T cell count ≥ 350 cells/mm^3
808 cells/mm^3
n=99 Participants
HIV-1 RNA < 50 copies/mL obtained within 60 days prior to study entry
9 Participants
n=99 Participants
on stable 3-antiretroviral medications with no changes for at least 4 weeks
9 Participants
n=99 Participants

PRIMARY outcome

Timeframe: 15 months

Population: No data was collected.

Change in the number of infectious units per million resting CD4+ T cells (IUPM) from baseline to the end of study treatment, as measured by the Quantitative Viral Outgrowth Assay (QVOA).

Outcome measures

Outcome data not reported

SECONDARY outcome

Timeframe: 15 months

Population: No data was collected.

Correlation coefficient between measures of the latent HIV reservoir by QVOA and measures of the latent HIV reservoir by the intact proviral DNA assay.

Outcome measures

Outcome data not reported

SECONDARY outcome

Timeframe: 15 months

Population: No data was collected.

Correlation coefficient between measures of the latent HIV reservoir by QVOA and measures of the latent HIV reservoir by the Tat-rev inducible limiting dilution assay (TILDA).

Outcome measures

Outcome data not reported

SECONDARY outcome

Timeframe: 15 months

Population: No data was collected.

Correlation coefficient between measures of the latent HIV reservoir by QVOA and measures of the latent HIV reservoir by the Envelope Detection by Induced Transcription-based Sequencing (EDITS).

Outcome measures

Outcome data not reported

SECONDARY outcome

Timeframe: 15 months

Population: No data was collected.

Number of inducible cell-associated HIV mRNA copies per million CD4+ T cells at the beginning and end of rIL2 cycles 1, 4, and 8.

Outcome measures

Outcome data not reported

SECONDARY outcome

Timeframe: baseline, day 7

Plasma HIV RNA copies/mL by PCR at the beginning and end of rIL2 cycle 1.

Outcome measures

Outcome measures
Measure
IL2 Treatment
n=9 Participants
Subcutaneous recombinant interleukin-2 (rIL2), 5 MIU twice daily for four consecutive days(cycle) every 8 weeks for 8 cycles, in addition to combination antiretroviral therapy. Recombinant Interleukin-2: Subcutaneous recombinant interleukin-2 (rIL2), 5 MIU twice daily for four consecutive days(cycle) every 8 weeks for 8 cycles
Plasma HIV RNA During rIL2 Exposure
baseline
10 RNA copies/mL
Standard Deviation 0
Plasma HIV RNA During rIL2 Exposure
day 7
298 RNA copies/mL
Standard Deviation 522

SECONDARY outcome

Timeframe: baseline, day 7

Change in the percent of NK cells expressing CD16+CD56+ by flow cytometry from baseline to the end of study treatment.

Outcome measures

Outcome measures
Measure
IL2 Treatment
n=9 Participants
Subcutaneous recombinant interleukin-2 (rIL2), 5 MIU twice daily for four consecutive days(cycle) every 8 weeks for 8 cycles, in addition to combination antiretroviral therapy. Recombinant Interleukin-2: Subcutaneous recombinant interleukin-2 (rIL2), 5 MIU twice daily for four consecutive days(cycle) every 8 weeks for 8 cycles
Natural Killer (NK) Cell Phenotype During rIL2 Exposure
baseline
28.8 percentage of cells
Interval 19.8 to 37.5
Natural Killer (NK) Cell Phenotype During rIL2 Exposure
day 7
35.8 percentage of cells
Interval 30.9 to 39.2

SECONDARY outcome

Timeframe: baseline, day 7

Change in the percent of NK cells expressing CD56+CD16- by flow cytometry from baseline to the end of study treatment.

Outcome measures

Outcome measures
Measure
IL2 Treatment
n=9 Participants
Subcutaneous recombinant interleukin-2 (rIL2), 5 MIU twice daily for four consecutive days(cycle) every 8 weeks for 8 cycles, in addition to combination antiretroviral therapy. Recombinant Interleukin-2: Subcutaneous recombinant interleukin-2 (rIL2), 5 MIU twice daily for four consecutive days(cycle) every 8 weeks for 8 cycles
Natural Killer (NK) Cell Phenotype During rIL2 Exposure
baseline
4.5 percentage of cells
Interval 3.1 to 5.2
Natural Killer (NK) Cell Phenotype During rIL2 Exposure
day 7
12.7 percentage of cells
Interval 8.3 to 17.1

Adverse Events

IL2 Treatment

Serious events: 1 serious events
Other events: 2 other events
Deaths: 0 deaths

Serious adverse events

Serious adverse events
Measure
IL2 Treatment
n=9 participants at risk
Subcutaneous recombinant interleukin-2 (rIL2), 5 MIU twice daily for four consecutive days(cycle) every 8 weeks for 8 cycles, in addition to combination antiretroviral therapy. Recombinant Interleukin-2: Subcutaneous recombinant interleukin-2 (rIL2), 5 MIU twice daily for four consecutive days(cycle) every 8 weeks for 8 cycles
Vascular disorders
suspected capillary leak syndrome
11.1%
1/9 • Number of events 1 • Adverse event data were collected until the study was terminated. This period was the time between enrollment into the study and study termination. The duration ranged from 8.3 months to 14.6 months,.
Total number of participants at risk for All-Cause Mortality is 9. All-Cause mortality was zero.

Other adverse events

Other adverse events
Measure
IL2 Treatment
n=9 participants at risk
Subcutaneous recombinant interleukin-2 (rIL2), 5 MIU twice daily for four consecutive days(cycle) every 8 weeks for 8 cycles, in addition to combination antiretroviral therapy. Recombinant Interleukin-2: Subcutaneous recombinant interleukin-2 (rIL2), 5 MIU twice daily for four consecutive days(cycle) every 8 weeks for 8 cycles
Endocrine disorders
biochemical hypothyroidism
22.2%
2/9 • Number of events 2 • Adverse event data were collected until the study was terminated. This period was the time between enrollment into the study and study termination. The duration ranged from 8.3 months to 14.6 months,.
Total number of participants at risk for All-Cause Mortality is 9. All-Cause mortality was zero.

Additional Information

Michael M. Lederman MD

Case Western Reserve University

Phone: 2168448786

Results disclosure agreements

  • Principal investigator is a sponsor employee
  • Publication restrictions are in place