Trial Outcomes & Findings for Discovery of Sirolimus Sensitive Biomarkers in Blood (NCT NCT03304678)

NCT ID: NCT03304678

Last Updated: 2026-02-18

Results Overview

Plasma from Lymphangioleiomyomatosis (LAM) participants was profiled using the aptamer-based Somascan 11k assay (Somalogic). The primary outcome is the number of plasma proteins that exhibit a statistically significant change from each participant's baseline following sirolimus administration, defined by pre-specified multiplicity-adjusted thresholds (e.g., FDR q\<0.05 with absolute log2 fold-change ≥0.5), representing candidate sirolimus-sensitive biomarkers.

Recruitment status

COMPLETED

Study phase

PHASE2

Target enrollment

33 participants

Primary outcome timeframe

Day 0 (pre-treatment, 1 hour post-Sirolimus, 23 hours post-Sirolimus), Month 3 (23 hours post-Sirolimus, 1 hour post next dose), Month 9 (23 hours post-Sirolimus, 1 hour post next dose)

Results posted on

2026-02-18

Participant Flow

Participant milestones

Participant milestones
Measure
Participants Diagnosed With Lymphangioleiomyomatosis Receiving Sirolimus
Participants diagnosed with Lymphangioleiomyomatosis (LAM) will be administered Sirolimus 2 mg by mouth daily as prescribed by treating physician will be referred to the NIH Clinical Center for these studies.
Overall Study
STARTED
33
Overall Study
COMPLETED
24
Overall Study
NOT COMPLETED
9

Reasons for withdrawal

Reasons for withdrawal
Measure
Participants Diagnosed With Lymphangioleiomyomatosis Receiving Sirolimus
Participants diagnosed with Lymphangioleiomyomatosis (LAM) will be administered Sirolimus 2 mg by mouth daily as prescribed by treating physician will be referred to the NIH Clinical Center for these studies.
Overall Study
Physician Decision
5
Overall Study
Adverse Event
1
Overall Study
Non-compliance
3

Baseline Characteristics

Discovery of Sirolimus Sensitive Biomarkers in Blood

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
Participants Diagnosed With Lymphangioleiomyomatosis Receiving Sirolimus
n=33 Participants
Participants diagnosed with Lymphangioleiomyomatosis (LAM) will be administered Sirolimus 2 mg by mouth daily as prescribed by treating physician will be referred to the NIH Clinical Center for these studies.
Age, Categorical
<=18 years
0 Participants
n=4 Participants
Age, Categorical
Between 18 and 65 years
33 Participants
n=4 Participants
Age, Categorical
>=65 years
0 Participants
n=4 Participants
Sex: Female, Male
Female
33 Participants
n=4 Participants
Sex: Female, Male
Male
0 Participants
n=4 Participants
Ethnicity (NIH/OMB)
Hispanic or Latino
0 Participants
n=4 Participants
Ethnicity (NIH/OMB)
Not Hispanic or Latino
32 Participants
n=4 Participants
Ethnicity (NIH/OMB)
Unknown or Not Reported
1 Participants
n=4 Participants
Race (NIH/OMB)
American Indian or Alaska Native
0 Participants
n=4 Participants
Race (NIH/OMB)
Asian
3 Participants
n=4 Participants
Race (NIH/OMB)
Native Hawaiian or Other Pacific Islander
0 Participants
n=4 Participants
Race (NIH/OMB)
Black or African American
3 Participants
n=4 Participants
Race (NIH/OMB)
White
25 Participants
n=4 Participants
Race (NIH/OMB)
More than one race
1 Participants
n=4 Participants
Race (NIH/OMB)
Unknown or Not Reported
1 Participants
n=4 Participants
Region of Enrollment
United States
33 participants
n=4 Participants

PRIMARY outcome

Timeframe: Day 0 (pre-treatment, 1 hour post-Sirolimus, 23 hours post-Sirolimus), Month 3 (23 hours post-Sirolimus, 1 hour post next dose), Month 9 (23 hours post-Sirolimus, 1 hour post next dose)

Population: Data from 20 participants were analyzed, as these individuals had plasma collections completed at all required timepoints.

Plasma from Lymphangioleiomyomatosis (LAM) participants was profiled using the aptamer-based Somascan 11k assay (Somalogic). The primary outcome is the number of plasma proteins that exhibit a statistically significant change from each participant's baseline following sirolimus administration, defined by pre-specified multiplicity-adjusted thresholds (e.g., FDR q\<0.05 with absolute log2 fold-change ≥0.5), representing candidate sirolimus-sensitive biomarkers.

Outcome measures

Outcome measures
Measure
Participant With LAM Receiving Sirolimus
n=10000 plasma proteins
Participants with Lymphangioleiomyomatosis (LAM) will receive Sirolimus 2 mg by mouth daily prescribed by treating physicians.
Number of Sirolimus-Sensitive Plasma Protein Biomarkers in Lymphangioleiomyomatosis (LAM) Participants
662 plasma proteins

Adverse Events

Participants Diagnosed With Lymphangioleiomyomatosis Receiving Sirolimus

Serious events: 0 serious events
Other events: 1 other events
Deaths: 0 deaths

Serious adverse events

Adverse event data not reported

Other adverse events

Other adverse events
Measure
Participants Diagnosed With Lymphangioleiomyomatosis Receiving Sirolimus
n=32 participants at risk
Participants diagnosed with Lymphangioleiomyomatosis (LAM) will be administered Sirolimus 2 mg by mouth daily as prescribed by treating physician will be referred to the NIH Clinical Center for these studies.
Respiratory, thoracic and mediastinal disorders
Pneumothorax
3.1%
1/32 • Number of events 1 • Up to 9 months

Additional Information

Joel Moss, M.D., Ph.D.

National Heart, Lung, and Blood Institute (NHLBI)

Phone: 301.496.1597

Results disclosure agreements

  • Principal investigator is a sponsor employee
  • Publication restrictions are in place