Trial Outcomes & Findings for P53 Mutational Status and cf HPV DNA for the Management of HPV-associated OPSCC (NCT NCT03077243)

NCT ID: NCT03077243

Last Updated: 2024-05-02

Results Overview

PFS was assessed as the time from the first day of chemoradiation therapy (CRT) until disease progression. Disease progression was defined as biopsy proven tumor cells. Positron emission tomography / computerized tomography (PET/CT) was performed at week 10-16 (optimally at week 12) after completion of therapy. Biopsies were performed for subjects with imaging or clinical exam results suspicious for tumor. Clinical follow-up occurred and chest imaging was performed during the follow-up.

Recruitment status

COMPLETED

Study phase

PHASE2

Target enrollment

195 participants

Primary outcome timeframe

Two years after completion of the treatment

Results posted on

2024-05-02

Participant Flow

Participants were recruited from 08/25/2016 through 12/11/2020 at 4 cancer centers in the United States.

A total of 195 subjects consented and started the study. Research at two study sites terminated early. Each site collected and stored the research data for its participants in its own systems. The validity of existing, sequestered data from sites 3 and 4 cannot be confirmed and may not be reliable. Therefore, arm/group and follow-up data related to sixty-nine subjects are not included and 126 subjects with arm/group and follow-up were presented.

Participant milestones

Participant milestones
Measure
Group A
Subjects with oropharyngeal squamous cell carcinoma have more than 10 years of smoking history, without p53 mutation and receiving 60Gy radiotherapy with or without chemotherapy.
Group B
Subjects with oropharyngeal squamous cell carcinoma have equal to or less than 10 years of smoking history, without p53 mutation and receiving 60Gy radiotherapy with or without chemotherapy
Group C
Subjects with oropharyngeal squamous cell carcinoma have more than 10 years of smoking history and with the p53 mutation and/or treating physicians' discretion receiving 70Gy radiotherapy with or without chemotherapy.
Group DataUnknown
Research at two study sites terminated early. Each site collected and stored the research data for its participants in its own systems. Demographic data and site assignment for all subjects were stored centrally. Therefore, arm/group and follow-up data related to sixty-nine subjects are missing.
Overall Study
STARTED
18
105
3
69
Overall Study
Assessment and Biopsy Was Done if Required at 2 Years After Treatment
11
51
2
0
Overall Study
COMPLETED
15
100
3
0
Overall Study
NOT COMPLETED
3
5
0
69

Reasons for withdrawal

Reasons for withdrawal
Measure
Group A
Subjects with oropharyngeal squamous cell carcinoma have more than 10 years of smoking history, without p53 mutation and receiving 60Gy radiotherapy with or without chemotherapy.
Group B
Subjects with oropharyngeal squamous cell carcinoma have equal to or less than 10 years of smoking history, without p53 mutation and receiving 60Gy radiotherapy with or without chemotherapy
Group C
Subjects with oropharyngeal squamous cell carcinoma have more than 10 years of smoking history and with the p53 mutation and/or treating physicians' discretion receiving 70Gy radiotherapy with or without chemotherapy.
Group DataUnknown
Research at two study sites terminated early. Each site collected and stored the research data for its participants in its own systems. Demographic data and site assignment for all subjects were stored centrally. Therefore, arm/group and follow-up data related to sixty-nine subjects are missing.
Overall Study
Death
1
2
0
0
Overall Study
Withdrawal by Subject
1
3
0
0
Overall Study
Physician Decision
1
0
0
0
Overall Study
Research at two study sites terminated early
0
0
0
69

Baseline Characteristics

P53 Mutational Status and cf HPV DNA for the Management of HPV-associated OPSCC

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
Group A
n=18 Participants
Subjects with oropharyngeal squamous cell carcinoma have more than 10 years of smoking history, without p53 mutation and receiving 60Gy radiotherapy with or without chemotherapy.
Group B
n=105 Participants
Subjects with oropharyngeal squamous cell carcinoma have equal to or less than 10 years of smoking history, without p53 mutation and receiving 60Gy radiotherapy with or without chemotherapy.
Group C
n=3 Participants
Subjects with oropharyngeal squamous cell carcinoma have more than 10 years of smoking history and with the p53 mutation and/or treating physicians' discretion receiving 70Gy radiotherapy with or without chemotherapy.
Group Data Unknown
n=69 Participants
Research at two study sites terminated early. Each site collected and stored the research data for its participants in its own systems. Demographic data and site assignment for all subjects were stored centrally. Therefore, arm/group and follow-up data related to sixty-nine subjects are missing.
Total
n=195 Participants
Total of all reporting groups
Age, Continuous
62.72 years
STANDARD_DEVIATION 8.43 • n=39 Participants
59.89 years
STANDARD_DEVIATION 9.8 • n=41 Participants
61.66 years
STANDARD_DEVIATION 4.16 • n=35 Participants
64.5 years
STANDARD_DEVIATION 9.74 • n=31 Participants
60.86 years
STANDARD_DEVIATION 9.61 • n=146 Participants
Sex: Female, Male
Female
1 Participants
n=39 Participants
12 Participants
n=41 Participants
3 Participants
n=35 Participants
3 Participants
n=31 Participants
19 Participants
n=146 Participants
Sex: Female, Male
Male
17 Participants
n=39 Participants
93 Participants
n=41 Participants
0 Participants
n=35 Participants
66 Participants
n=31 Participants
176 Participants
n=146 Participants
Ethnicity (NIH/OMB)
Hispanic or Latino
0 Participants
n=39 Participants
3 Participants
n=41 Participants
0 Participants
n=35 Participants
2 Participants
n=31 Participants
5 Participants
n=146 Participants
Ethnicity (NIH/OMB)
Not Hispanic or Latino
16 Participants
n=39 Participants
99 Participants
n=41 Participants
3 Participants
n=35 Participants
67 Participants
n=31 Participants
185 Participants
n=146 Participants
Ethnicity (NIH/OMB)
Unknown or Not Reported
2 Participants
n=39 Participants
3 Participants
n=41 Participants
0 Participants
n=35 Participants
0 Participants
n=31 Participants
5 Participants
n=146 Participants
Race (NIH/OMB)
American Indian or Alaska Native
0 Participants
n=39 Participants
0 Participants
n=41 Participants
0 Participants
n=35 Participants
0 Participants
n=31 Participants
0 Participants
n=146 Participants
Race (NIH/OMB)
Asian
0 Participants
n=39 Participants
1 Participants
n=41 Participants
0 Participants
n=35 Participants
0 Participants
n=31 Participants
1 Participants
n=146 Participants
Race (NIH/OMB)
Native Hawaiian or Other Pacific Islander
0 Participants
n=39 Participants
0 Participants
n=41 Participants
0 Participants
n=35 Participants
0 Participants
n=31 Participants
0 Participants
n=146 Participants
Race (NIH/OMB)
Black or African American
0 Participants
n=39 Participants
4 Participants
n=41 Participants
1 Participants
n=35 Participants
2 Participants
n=31 Participants
7 Participants
n=146 Participants
Race (NIH/OMB)
White
17 Participants
n=39 Participants
91 Participants
n=41 Participants
2 Participants
n=35 Participants
67 Participants
n=31 Participants
177 Participants
n=146 Participants
Race (NIH/OMB)
More than one race
0 Participants
n=39 Participants
0 Participants
n=41 Participants
0 Participants
n=35 Participants
0 Participants
n=31 Participants
0 Participants
n=146 Participants
Race (NIH/OMB)
Unknown or Not Reported
1 Participants
n=39 Participants
9 Participants
n=41 Participants
0 Participants
n=35 Participants
0 Participants
n=31 Participants
10 Participants
n=146 Participants
Region of Enrollment
United States
18 participants
n=39 Participants
105 participants
n=41 Participants
3 participants
n=35 Participants
69 participants
n=31 Participants
195 participants
n=146 Participants

PRIMARY outcome

Timeframe: Two years after completion of the treatment

Population: Subjects completed the study procedures including treatment, PET/CT, and biopsy when required, and followed up 2 years after completion of the treatment. Research at two study sites terminated early. Each site collected and stored the research data for its participants in its own systems. The validity of existing, sequestered outcome data from sites 3 and 4 cannot be confirmed and may not be reliable. Therefore, arm/group and follow-up data related to sixty-nine subjects are not included.

PFS was assessed as the time from the first day of chemoradiation therapy (CRT) until disease progression. Disease progression was defined as biopsy proven tumor cells. Positron emission tomography / computerized tomography (PET/CT) was performed at week 10-16 (optimally at week 12) after completion of therapy. Biopsies were performed for subjects with imaging or clinical exam results suspicious for tumor. Clinical follow-up occurred and chest imaging was performed during the follow-up.

Outcome measures

Outcome measures
Measure
Group A
n=11 Participants
Subjects with oropharyngeal squamous cell carcinoma have more than 10 years of smoking history, without p53 mutation and receiving 60Gy radiotherapy with or without chemotherapy.
Group B
n=51 Participants
Subjects with oropharyngeal squamous cell carcinoma have equal to or less than 10 years of smoking history, without p53 mutation and receiving 60Gy radiotherapy with or without chemotherapy.
Group C
n=2 Participants
Subjects with oropharyngeal squamous cell carcinoma have more than 10 years of smoking history and with the p53 mutation and/or treating physicians' discretion receiving 70Gy radiotherapy with or without chemotherapy.
Progression Free Survival (PFS)
Biopsy proven relapse was found
3 Participants
6 Participants
0 Participants
Progression Free Survival (PFS)
Biopsy proven relapse was not found
8 Participants
45 Participants
2 Participants

SECONDARY outcome

Timeframe: Baseline

Population: Subjects started the treatment and provided specimens for plasma circulating free HPV DNA analysis at baseline. Research at two study sites terminated early. Each site collected and stored the research data for its participants in its own systems. The validity of existing, sequestered outcome data from sites 3 and 4 cannot be confirmed and may not be reliable. Therefore, arm/group and follow-up data related to sixty-nine subjects are not included.

The number of subjects who have or do not have plasma circulating free Human papillomavirus Deoxyribonucleic acid (HPV- DNA) was tabulated.

Outcome measures

Outcome measures
Measure
Group A
n=14 Participants
Subjects with oropharyngeal squamous cell carcinoma have more than 10 years of smoking history, without p53 mutation and receiving 60Gy radiotherapy with or without chemotherapy.
Group B
n=63 Participants
Subjects with oropharyngeal squamous cell carcinoma have equal to or less than 10 years of smoking history, without p53 mutation and receiving 60Gy radiotherapy with or without chemotherapy.
Group C
n=2 Participants
Subjects with oropharyngeal squamous cell carcinoma have more than 10 years of smoking history and with the p53 mutation and/or treating physicians' discretion receiving 70Gy radiotherapy with or without chemotherapy.
Number of Participants With Plasma Circulating Free DNA -Baseline
HPV (+)
13 Participants
59 Participants
2 Participants
Number of Participants With Plasma Circulating Free DNA -Baseline
HPV (-)
1 Participants
4 Participants
0 Participants

SECONDARY outcome

Timeframe: 3 months after completion of the treatment

Population: Subjects completed the study treatment and provided specimens for plasma circulating free HPV DNA analysis at 3 months after the completion of radiotherapy. Research at two study sites terminated early. Each site collected and stored the research data for its participants in its own systems. The validity of existing, sequestered outcome data from sites 3 and 4 cannot be confirmed and may not be reliable. Therefore, arm/group and follow-up data related to sixty-nine subjects are not included.

The number of subjects who have or do not have plasma circulating free Human papillomavirus Deoxyribonucleic acid (HPV- DNA) was tabulated.

Outcome measures

Outcome measures
Measure
Group A
n=13 Participants
Subjects with oropharyngeal squamous cell carcinoma have more than 10 years of smoking history, without p53 mutation and receiving 60Gy radiotherapy with or without chemotherapy.
Group B
n=59 Participants
Subjects with oropharyngeal squamous cell carcinoma have equal to or less than 10 years of smoking history, without p53 mutation and receiving 60Gy radiotherapy with or without chemotherapy.
Group C
n=1 Participants
Subjects with oropharyngeal squamous cell carcinoma have more than 10 years of smoking history and with the p53 mutation and/or treating physicians' discretion receiving 70Gy radiotherapy with or without chemotherapy.
Number of Participants With Plasma Circulating Free DNA -3months
HPV (+)
3 Participants
19 Participants
0 Participants
Number of Participants With Plasma Circulating Free DNA -3months
HPV (-)
10 Participants
40 Participants
1 Participants

SECONDARY outcome

Timeframe: 1 year after completion of the treatment

Population: Subjects completed the study treatment and provided specimens for plasma circulating free HPV DNA analysis 1 year after the completion of radiotherapy. Research at two study sites terminated early. Each site collected and stored the research data for its participants in its own systems. The validity of existing, sequestered outcome data from sites 3 and 4 cannot be confirmed and may not be reliable. Therefore, arm/group and follow-up data related to sixty-nine subjects are not included.

The number of subjects who have or do not have plasma circulating free Human papillomavirus Deoxyribonucleic acid (HPV- DNA) was tabulated.

Outcome measures

Outcome measures
Measure
Group A
n=7 Participants
Subjects with oropharyngeal squamous cell carcinoma have more than 10 years of smoking history, without p53 mutation and receiving 60Gy radiotherapy with or without chemotherapy.
Group B
n=51 Participants
Subjects with oropharyngeal squamous cell carcinoma have equal to or less than 10 years of smoking history, without p53 mutation and receiving 60Gy radiotherapy with or without chemotherapy.
Group C
Subjects with oropharyngeal squamous cell carcinoma have more than 10 years of smoking history and with the p53 mutation and/or treating physicians' discretion receiving 70Gy radiotherapy with or without chemotherapy.
Number of Participants With Plasma Circulating Free DNA -1 Year
HPV (+)
2 Participants
10 Participants
0 Participants
Number of Participants With Plasma Circulating Free DNA -1 Year
HPV (-)
5 Participants
41 Participants
0 Participants

SECONDARY outcome

Timeframe: 2 years after completion of the treatment

Population: Subjects completed the study treatment and provided specimens for plasma circulating free HPV DNA analysis 2 years after the completion of radiotherapy. Research at two study sites terminated early. Each site collected and stored the research data for its participants in its own systems. The validity of existing, sequestered outcome data from sites 3 and 4 cannot be confirmed and may not be reliable. Therefore, arm/group and follow-up data related to sixty-nine subjects are not included.

The number of subjects who have or do not have plasma circulating free Human papillomavirus Deoxyribonucleic acid (HPV- DNA) was tabulated.

Outcome measures

Outcome measures
Measure
Group A
n=6 Participants
Subjects with oropharyngeal squamous cell carcinoma have more than 10 years of smoking history, without p53 mutation and receiving 60Gy radiotherapy with or without chemotherapy.
Group B
n=39 Participants
Subjects with oropharyngeal squamous cell carcinoma have equal to or less than 10 years of smoking history, without p53 mutation and receiving 60Gy radiotherapy with or without chemotherapy.
Group C
n=1 Participants
Subjects with oropharyngeal squamous cell carcinoma have more than 10 years of smoking history and with the p53 mutation and/or treating physicians' discretion receiving 70Gy radiotherapy with or without chemotherapy.
Number of Participants With Plasma Circulating Free DNA -2 Year
HPV (+)
2 Participants
16 Participants
0 Participants
Number of Participants With Plasma Circulating Free DNA -2 Year
HPV (-)
4 Participants
23 Participants
1 Participants

SECONDARY outcome

Timeframe: 2 years after completion of the treatment

Population: Subjects completed the study procedures including treatment, PET/CT, and biopsy when required, and followed up 2 years after completion of the treatment. Research at two study sites terminated early. Each site collected and stored the research data for its participants in its own systems. The validity of existing, sequestered outcome data from sites 3 and 4 cannot be confirmed and may not be reliable. Therefore, arm/group and follow-up data related to sixty-nine subjects are not included.

The local control rate is defined as the total disappearance of the primary tumor without any local recurrence. Local recurrence was defined as biopsy-proven tumor cells in the primary tumor region. Biopsy was performed for a subject with a positive positron emission tomography / computerized tomography scan and /or clinical discretion of the surgeon 16 weeks after completion of the treatment.

Outcome measures

Outcome measures
Measure
Group A
n=11 Participants
Subjects with oropharyngeal squamous cell carcinoma have more than 10 years of smoking history, without p53 mutation and receiving 60Gy radiotherapy with or without chemotherapy.
Group B
n=51 Participants
Subjects with oropharyngeal squamous cell carcinoma have equal to or less than 10 years of smoking history, without p53 mutation and receiving 60Gy radiotherapy with or without chemotherapy.
Group C
n=2 Participants
Subjects with oropharyngeal squamous cell carcinoma have more than 10 years of smoking history and with the p53 mutation and/or treating physicians' discretion receiving 70Gy radiotherapy with or without chemotherapy.
Local Control Rate
local relapse
0 Participants
0 Participants
0 Participants
Local Control Rate
No local relapse
11 Participants
51 Participants
2 Participants

SECONDARY outcome

Timeframe: 2 years post-CRT

Population: Subjects completed the study procedures including treatment, PET/CT, and biopsy when required, and followed up 2 years after completion of the treatment. Research at two study sites terminated early. Each site collected and stored the research data for its participants in its own systems. The validity of existing, sequestered outcome data from sites 3 and 4 cannot be confirmed and may not be reliable. Therefore, arm/group and follow-up data related to sixty-nine subjects are not included.

Regional control rate is defined as the total disease disappearance of the related lymph node metastases without any lymph node recurrence. Regional recurrence was defined as biopsy proven tumor cells in related lymph nodes. Biopsy was performed for a subject with a positive positron emission tomography / computerized tomography scan and /or clinical discretion of the surgeon 16 weeks after completion of the treatment.

Outcome measures

Outcome measures
Measure
Group A
n=11 Participants
Subjects with oropharyngeal squamous cell carcinoma have more than 10 years of smoking history, without p53 mutation and receiving 60Gy radiotherapy with or without chemotherapy.
Group B
n=51 Participants
Subjects with oropharyngeal squamous cell carcinoma have equal to or less than 10 years of smoking history, without p53 mutation and receiving 60Gy radiotherapy with or without chemotherapy.
Group C
n=2 Participants
Subjects with oropharyngeal squamous cell carcinoma have more than 10 years of smoking history and with the p53 mutation and/or treating physicians' discretion receiving 70Gy radiotherapy with or without chemotherapy.
Regional Control Rate
Biopsy proven regional relapse was found
1 Participants
2 Participants
0 Participants
Regional Control Rate
Biopsy proven regional relapse was not found
10 Participants
49 Participants
2 Participants

SECONDARY outcome

Timeframe: 2 years after completion of the treatment

Population: Subjects completed the study procedures including treatment, PET/CT, and biopsy when required, and followed up 2 years after completion of the treatment. Research at two study sites terminated early. Each site collected and stored the research data for its participants in its own systems. The validity of existing, sequestered outcome data from sites 3 and 4 cannot be confirmed and may not be reliable. Therefore, arm/group and follow-up data related to sixty-nine subjects are not included.

Local-regional control rate is defined as the total disappearance of the primary tumor and related lymph node metastases without any recurrence. Local-regional recurrence was defined as biopsy-proven tumor cells in the primary tumor region and/or related lymph nodes. Biopsy was performed for a subject with a positive positron emission tomography / computerized tomography scan and /or clinical discretion of the surgeon 16 weeks after completion of the treatment.

Outcome measures

Outcome measures
Measure
Group A
n=11 Participants
Subjects with oropharyngeal squamous cell carcinoma have more than 10 years of smoking history, without p53 mutation and receiving 60Gy radiotherapy with or without chemotherapy.
Group B
n=51 Participants
Subjects with oropharyngeal squamous cell carcinoma have equal to or less than 10 years of smoking history, without p53 mutation and receiving 60Gy radiotherapy with or without chemotherapy.
Group C
n=2 Participants
Subjects with oropharyngeal squamous cell carcinoma have more than 10 years of smoking history and with the p53 mutation and/or treating physicians' discretion receiving 70Gy radiotherapy with or without chemotherapy.
Local-regional Control Rate
Biopsy proven local or regional relapse was found
1 Participants
2 Participants
0 Participants
Local-regional Control Rate
Biopsy proven local or regional relapse was not found
10 Participants
49 Participants
2 Participants

SECONDARY outcome

Timeframe: Two years after completion of the treatment

Population: Subjects completed the study procedures including treatment, PET/CT, and biopsy when required, and followed up 2 years after completion of the treatment. Research at two study sites terminated early. Each site collected and stored the research data for its participants in its own systems. The validity of existing, sequestered outcome data from sites 3 and 4 cannot be confirmed and may not be reliable. Therefore, arm/group and follow-up data related to sixty-nine subjects are not included.

Distant metastasis-free survival is defined as the time from the first day of the study treatment to the date of disease spreads while subjects are alive. Distant metastasis-free survival is defined as no disease outside of the primary tumor and related lymph node metastases. Distant metastasis was defined as biopsy-proven tumor cells outside of the primary tumor and related lymph node metastases. Biopsy was performed for a subject with a positive positron emission tomography / computerized tomography scan and /or clinical discretion of the surgeon 16 weeks after completion of the treatment.

Outcome measures

Outcome measures
Measure
Group A
n=11 Participants
Subjects with oropharyngeal squamous cell carcinoma have more than 10 years of smoking history, without p53 mutation and receiving 60Gy radiotherapy with or without chemotherapy.
Group B
n=51 Participants
Subjects with oropharyngeal squamous cell carcinoma have equal to or less than 10 years of smoking history, without p53 mutation and receiving 60Gy radiotherapy with or without chemotherapy.
Group C
n=2 Participants
Subjects with oropharyngeal squamous cell carcinoma have more than 10 years of smoking history and with the p53 mutation and/or treating physicians' discretion receiving 70Gy radiotherapy with or without chemotherapy.
Distant Metastasis Free Survival
Biopsy proven distant metastasis was found
2 Participants
4 Participants
0 Participants
Distant Metastasis Free Survival
Biopsy proven distant metastasis was not found
9 Participants
47 Participants
2 Participants

SECONDARY outcome

Timeframe: Up to 2 years after completion of treatment

Population: Research at two study sites terminated early. Each site collected and stored the research data for its participants in its own systems. The validity of existing, sequestered outcome data from sites 3 and 4 cannot be confirmed and may not be reliable. Therefore, arm/group and follow-up data related to sixty-nine subjects are not included.

The overall survival rate is defined as the time from the first day of the study treatment to the date of death for any cause. Subjects who have not had an event will be censored at the date of the last assessment documenting the subject was alive.

Outcome measures

Outcome measures
Measure
Group A
n=12 Participants
Subjects with oropharyngeal squamous cell carcinoma have more than 10 years of smoking history, without p53 mutation and receiving 60Gy radiotherapy with or without chemotherapy.
Group B
n=55 Participants
Subjects with oropharyngeal squamous cell carcinoma have equal to or less than 10 years of smoking history, without p53 mutation and receiving 60Gy radiotherapy with or without chemotherapy.
Group C
n=2 Participants
Subjects with oropharyngeal squamous cell carcinoma have more than 10 years of smoking history and with the p53 mutation and/or treating physicians' discretion receiving 70Gy radiotherapy with or without chemotherapy.
Overall Survival Rate
Death
1 Participants
4 Participants
0 Participants
Overall Survival Rate
Alive
11 Participants
51 Participants
2 Participants

Adverse Events

Group A

Serious events: 0 serious events
Other events: 0 other events
Deaths: 1 deaths

Group B

Serious events: 0 serious events
Other events: 0 other events
Deaths: 4 deaths

Group C

Serious events: 0 serious events
Other events: 0 other events
Deaths: 0 deaths

Group DataUnknown

Serious events: 0 serious events
Other events: 0 other events
Deaths: 0 deaths

Serious adverse events

Adverse event data not reported

Other adverse events

Adverse event data not reported

Additional Information

Melahat G. Canter MD MS Clinical Trial Analyst

University of North Carolina Lineberger Comprehensive Cancer Center

Phone: (919) 962-0000

Results disclosure agreements

  • Principal investigator is a sponsor employee LCCC1612 is a multi-site study. Three of the four site investigators are not employees of the Sponsor. Restrictions to disclosure do not apply due to circumstances of non-compliance. Study data was sequestered, data is not accessible for further analysis or publication.
  • Publication restrictions are in place

Restriction type: OTHER