Trial Outcomes & Findings for Interaction of Melatonin and MTNR1B Genotype on Glucose Control - Study 1 (NCT NCT02642640)

NCT ID: NCT02642640

Last Updated: 2026-02-12

Results Overview

Disposition index is determined by frequently sampled intravenous glucose tolerance test. It is a composite measure of β-cell function adjusted for insulin sensitivity-was calculated as the product of insulin sensitivity and insulin secretion indices. High value means stronger β-cell function. Theoretical minimum is 0, no theoretical maximum.

Recruitment status

COMPLETED

Study phase

NA

Target enrollment

23 participants

Primary outcome timeframe

During frequently sampled intravenous glucose tolerance test after melatonin/placebo administration on Day 2/4 (melatonin/placebo order randomized) in the laboratory visit

Results posted on

2026-02-12

Participant Flow

Ambulatory phase (2/3 weeks), in which participants follow a self-selected Sleep-Wake cycle as well as eat pre-prepared, standardized meals for 3 days leading into the 5-day in-lab stay.

Participant milestones

Participant milestones
Measure
Placebo-melatonin
subjects will receive placebo first and melatonin second
Melatonin-placebo
subjects will receive melatonin first and placebo second
Pre-in-lab phase
STARTED
12
11
Pre-in-lab phase
COMPLETED
12
11
Pre-in-lab phase
NOT COMPLETED
0
0
Intervention 1
STARTED
12
11
Intervention 1
COMPLETED
11
11
Intervention 1
NOT COMPLETED
1
0
Washout
STARTED
11
11
Washout
COMPLETED
11
11
Washout
NOT COMPLETED
0
0
Intervention 2
STARTED
11
11
Intervention 2
COMPLETED
11
10
Intervention 2
NOT COMPLETED
0
1

Reasons for withdrawal

Withdrawal data not reported

Baseline Characteristics

Interaction of Melatonin and MTNR1B Genotype on Glucose Control - Study 1

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
All Study Participants
n=21 Participants
All participants were randomized to receive all interventions, so all participants are combined into one Group "All Study Participants".
Age, Continuous
30.1 years
STANDARD_DEVIATION 10.1 • n=41 Participants
Sex: Female, Male
Female
8 Participants
n=41 Participants
Sex: Female, Male
Male
13 Participants
n=41 Participants
Race (NIH/OMB)
American Indian or Alaska Native
0 Participants
n=41 Participants
Race (NIH/OMB)
Asian
0 Participants
n=41 Participants
Race (NIH/OMB)
Native Hawaiian or Other Pacific Islander
0 Participants
n=41 Participants
Race (NIH/OMB)
Black or African American
0 Participants
n=41 Participants
Race (NIH/OMB)
White
21 Participants
n=41 Participants
Race (NIH/OMB)
More than one race
0 Participants
n=41 Participants
Race (NIH/OMB)
Unknown or Not Reported
0 Participants
n=41 Participants
Ethnicity (NIH/OMB)
Hispanic or Latino
1 Participants
n=41 Participants
Ethnicity (NIH/OMB)
Not Hispanic or Latino
20 Participants
n=41 Participants
Ethnicity (NIH/OMB)
Unknown or Not Reported
0 Participants
n=41 Participants
HbA1c
5.2 %
STANDARD_DEVIATION 0.28 • n=41 Participants

PRIMARY outcome

Timeframe: During frequently sampled intravenous glucose tolerance test after melatonin/placebo administration on Day 2/4 (melatonin/placebo order randomized) in the laboratory visit

Population: 1 participant excluded at melatonin treatment because of a technical difficulty during one of the frequently sampled intravenous glucose tolerance tests

Disposition index is determined by frequently sampled intravenous glucose tolerance test. It is a composite measure of β-cell function adjusted for insulin sensitivity-was calculated as the product of insulin sensitivity and insulin secretion indices. High value means stronger β-cell function. Theoretical minimum is 0, no theoretical maximum.

Outcome measures

Outcome measures
Measure
Placebo
n=21 Participants
Placebo oral administration
Melatonin
n=20 Participants
Melatonin oral administration
Disposition Index
1314.7 index
Interval 1012.6 to 1707.0
984.5 index
Interval 687.0 to 1410.8

SECONDARY outcome

Timeframe: During frequently sampled intravenous glucose tolerance test after melatonin/placebo administration on Day 2/4 (melatonin/placebo order randomized) in the laboratory visit

First-phase beta-cell responsivity to glucose is determined by frequently sampled intravenous glucose tolerance test. It likely represents exocytosis of previously primed and docked insulin secretory granules (commonly called the readily releasable pool). It is given by the ratio between the incremental amount of C-peptide secreted during the first phase and the maximum increment of the plasma glucose concentration.High value means stronger β-cell responsivity. Theoretical minimum is 0, no theoretical maximum.

Outcome measures

Outcome measures
Measure
Placebo
n=21 Participants
Placebo oral administration
Melatonin
n=21 Participants
Melatonin oral administration
First-phase Beta-cell Responsivity
118.3 index
Interval 98.3 to 142.4
88.9 index
Interval 75.4 to 104.9

SECONDARY outcome

Timeframe: During frequently sampled intravenous glucose tolerance test after melatonin/placebo administration on Day 2/4 (melatonin/placebo order randomized) in the laboratory visit

Population: 1 participant excluded at melatonin treatment because of a technical difficulty during one of the frequently sampled intravenous glucose tolerance tests

Insulin sensitivity is determined by frequently sampled intravenous glucose tolerance test. It is defined in quantitative terms as the effect of insulin to catalyse the disappearance of glucose from plasma.High value indicates better insulin sensitivity. Theoretical minimum is 0, no theoretical maximum.

Outcome measures

Outcome measures
Measure
Placebo
n=21 Participants
Placebo oral administration
Melatonin
n=20 Participants
Melatonin oral administration
Insulin Sensitivity
4.7 [(mu/l)^-1.min^-1]
Interval 3.7 to 5.8
3.7 [(mu/l)^-1.min^-1]
Interval 2.5 to 5.3

SECONDARY outcome

Timeframe: During frequently sampled intravenous glucose tolerance test after melatonin/placebo administration on Day 2/4 (melatonin/placebo order randomized) in the laboratory visit

Population: 1 participant excluded at melatonin treatment because of a technical difficulty during one of the frequently sampled intravenous glucose tolerance tests

glucose 3-hour incremental AUC during frequently sampled intravenous glucose tolerance test. High value indicates higher glucose level during the test, which is bad. Theoretical minimum is 0, no theoretical maximum.

Outcome measures

Outcome measures
Measure
Placebo
n=21 Participants
Placebo oral administration
Melatonin
n=20 Participants
Melatonin oral administration
Glucose Tolerance
322.6 [mmol/l.min]
95% Confidence Interval 24.7 • Interval 296.5 to 350.9
329.4 [mmol/l.min]
95% Confidence Interval 12.7 • Interval 302.9 to 358.2

Adverse Events

All Intervention Combined

Serious events: 0 serious events
Other events: 2 other events
Deaths: 0 deaths

Serious adverse events

Adverse event data not reported

Other adverse events

Other adverse events
Measure
All Intervention Combined
n=23 participants at risk
Placebo or Melatonin oral administration on Day 2 or Day 4 of their in-laboratory stay (order randomized)
General disorders
Nausea
4.3%
1/23 • From admission to the in-laboratory until the discharge from the laboratory, up to 5 days
Adverse events were reported with both interventions combined. This is because the adverse events happened either before any intervention started or after both ended, it is hard to attribute the events to one specific intervention.
Surgical and medical procedures
Failed attempts to place IV lines
4.3%
1/23 • Number of events 1 • From admission to the in-laboratory until the discharge from the laboratory, up to 5 days
Adverse events were reported with both interventions combined. This is because the adverse events happened either before any intervention started or after both ended, it is hard to attribute the events to one specific intervention.

Additional Information

Frank AJL Scheer, Ph.D.

Brigham and Women's Hospital

Phone: 617-732-7014

Results disclosure agreements

  • Principal investigator is a sponsor employee
  • Publication restrictions are in place