Trial Outcomes & Findings for Investigation of the Safety and Tolerability of BSCT (Anti-nf-P2X7) 10% Ointment (NCT NCT02587819)

NCT ID: NCT02587819

Last Updated: 2016-04-14

Results Overview

Adverse events and any changes in physical examinations will be monitored, as described in the Code of Federal Regulations (CFR) Title 21 Part 312. In particular local cutaneous irritation including erythema, peeling, dryness, itching, and burning/ stinging that first occur during the study or represent a worsening from Baseline will be recorded as AEs.

Recruitment status

COMPLETED

Study phase

PHASE1

Target enrollment

21 participants

Primary outcome timeframe

8 weeks

Results posted on

2016-04-14

Participant Flow

Participant milestones

Participant milestones
Measure
Treatment With BSCT
BSCT (anti-nf-P2X7) 10% Ointment topically applied twice daily for 28 consecutive days
Overall Study
STARTED
21
Overall Study
Exposed
21
Overall Study
COMPLETED
19
Overall Study
NOT COMPLETED
2

Reasons for withdrawal

Reasons for withdrawal
Measure
Treatment With BSCT
BSCT (anti-nf-P2X7) 10% Ointment topically applied twice daily for 28 consecutive days
Overall Study
Adverse Event
1
Overall Study
Lost to Follow-up
1

Baseline Characteristics

Investigation of the Safety and Tolerability of BSCT (Anti-nf-P2X7) 10% Ointment

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
Treatment With BSCT
n=21 Participants
BSCT (anti-nf-P2X7) 10% Ointment topically applied twice daily for 28 consecutive days
Age, Continuous
65.0 years
STANDARD_DEVIATION 14.4 • n=99 Participants
Sex: Female, Male
Female
8 Participants
n=99 Participants
Sex: Female, Male
Male
13 Participants
n=99 Participants
Ethnicity (NIH/OMB)
Hispanic or Latino
0 Participants
n=99 Participants
Ethnicity (NIH/OMB)
Not Hispanic or Latino
21 Participants
n=99 Participants
Ethnicity (NIH/OMB)
Unknown or Not Reported
0 Participants
n=99 Participants
Race (NIH/OMB)
American Indian or Alaska Native
0 Participants
n=99 Participants
Race (NIH/OMB)
Asian
0 Participants
n=99 Participants
Race (NIH/OMB)
Native Hawaiian or Other Pacific Islander
0 Participants
n=99 Participants
Race (NIH/OMB)
Black or African American
0 Participants
n=99 Participants
Race (NIH/OMB)
White
21 Participants
n=99 Participants
Race (NIH/OMB)
More than one race
0 Participants
n=99 Participants
Race (NIH/OMB)
Unknown or Not Reported
0 Participants
n=99 Participants

PRIMARY outcome

Timeframe: 8 weeks

Population: All (21 of 21) subjects returned for safety and tolerability assessments at days 3, 8, 15 and 29 post-Baseline. 20 of 21 patients returned for final safety assessments was at 57 days post-Baseline.

Adverse events and any changes in physical examinations will be monitored, as described in the Code of Federal Regulations (CFR) Title 21 Part 312. In particular local cutaneous irritation including erythema, peeling, dryness, itching, and burning/ stinging that first occur during the study or represent a worsening from Baseline will be recorded as AEs.

Outcome measures

Outcome measures
Measure
Treatment With BSCT
n=21 Participants
BSCT (anti-nf-P2X7) 10% Ointment topically applied twice daily for 28 consecutive days post baseline to all subjects. All (21 of 21) subjects returned for safety and tolerability assessments at days 3, 8, 15 and 29 post-Baseline. 20 of 21 patients returned for final safety assessments at 57 days post-Baseline.
Incidence of Treatment-Emergent Adverse Events (Safety and Tolerability)
Subjects with any Serious Adverse Events
1 participants
Incidence of Treatment-Emergent Adverse Events (Safety and Tolerability)
Subjects with Treatment-emergent Adverse Event(s)
12 participants

PRIMARY outcome

Timeframe: 28 days

Population: At all timepoints, the serum concentration of sheep IgG was too low to be quantified in most of the subjects. One subject had measurable sheep IgG at 1 hr post dose at Visit 6 (EOT) and 3 other subjects had measurable sheep IgG at predose timepoints. In outcome measure below NA represents readings less than lower limit of quantification.

To determine PK, blood levels of sheep IgG were measured in samples collected at Visit 2 (Baseline), Visit 5, predose at Visit 6 (EOT), and then at 1 h, 2 h, and 4 h after the last dose of study medication.

Outcome measures

Outcome measures
Measure
Treatment With BSCT
n=21 Participants
BSCT (anti-nf-P2X7) 10% Ointment topically applied twice daily for 28 consecutive days post baseline to all subjects. All (21 of 21) subjects returned for safety and tolerability assessments at days 3, 8, 15 and 29 post-Baseline. 20 of 21 patients returned for final safety assessments at 57 days post-Baseline.
Pharmacokinetics - Measure Serum Concentration of Total Sheep IgG Using an ELISA.
Pre-dose - Baseline visit
NA ng/ml
All patients had levels of sheep IgG below limit of quantitation.
Pharmacokinetics - Measure Serum Concentration of Total Sheep IgG Using an ELISA.
Pre dose - Visit 5
NA ng/ml
Interval to 24.68
The majority of patients had levels of sheep IgG below limit of quantitation.
Pharmacokinetics - Measure Serum Concentration of Total Sheep IgG Using an ELISA.
Pre dose - Visit 6
NA ng/ml
Interval to 22.85
The majority of patients had levels of sheep IgG below limit of quantitation.
Pharmacokinetics - Measure Serum Concentration of Total Sheep IgG Using an ELISA.
2 hours post dose - Visit 6
NA ng/ml
All patients had levels of sheep IgG below limit of quantitation.
Pharmacokinetics - Measure Serum Concentration of Total Sheep IgG Using an ELISA.
4 hours post dose - Visit 6
NA ng/ml
All patients had levels of sheep IgG below limit of quantitation.
Pharmacokinetics - Measure Serum Concentration of Total Sheep IgG Using an ELISA.
1 hour post dose - Visit 6
NA ng/ml
Interval to 7.08
The majority of patients had levels of sheep IgG below limit of quantitation.

PRIMARY outcome

Timeframe: 8 weeks

Population: Anti sheep IgG antibody titres were measured from 21 subjects at screening and baseline, 20 subjects at Visit 6 (Day 29 EOT) and 19 subjects at Visit 8 (Day 57 follow up). The percentage of patients with detectable anti sheep antibodies is reported.

The active ingredient of BSCT is sheep IgG which may causes an immunogenic response if it enters the systemic circulation. To monitor this response patient blood samples collected at Screening, Visit 2 (Baseline), Visit 6 (EOT), and at Visit 8 (EOS) was tested for anti-sheep IgG antibodies (indicative of immune response against API).

Outcome measures

Outcome measures
Measure
Treatment With BSCT
n=21 Participants
BSCT (anti-nf-P2X7) 10% Ointment topically applied twice daily for 28 consecutive days post baseline to all subjects. All (21 of 21) subjects returned for safety and tolerability assessments at days 3, 8, 15 and 29 post-Baseline. 20 of 21 patients returned for final safety assessments at 57 days post-Baseline.
Pharmacokinetics - Measure Subject Antibody Response to the Active Pharmaceutical Ingredient Using an Indirect Fluorescent Immuno Assay.
Subjects with anti sheep IgG at screening
42.9 percentage of subjects
Pharmacokinetics - Measure Subject Antibody Response to the Active Pharmaceutical Ingredient Using an Indirect Fluorescent Immuno Assay.
Subjects with anti sheep IgG at Baseline
38.1 percentage of subjects
Pharmacokinetics - Measure Subject Antibody Response to the Active Pharmaceutical Ingredient Using an Indirect Fluorescent Immuno Assay.
Subjects with anti sheep IgG at visit 6
50 percentage of subjects
Pharmacokinetics - Measure Subject Antibody Response to the Active Pharmaceutical Ingredient Using an Indirect Fluorescent Immuno Assay.
Subjects with anti sheep IgG at visit 8
52.6 percentage of subjects

POST_HOC outcome

Timeframe: 28 days

Population: Final area of lesion was not recorded for one subject.

BCC lesion area was measured at Baseline and after 28 days treatment. Percantage change in lesion area was caculated.

Outcome measures

Outcome measures
Measure
Treatment With BSCT
n=20 Participants
BSCT (anti-nf-P2X7) 10% Ointment topically applied twice daily for 28 consecutive days post baseline to all subjects. All (21 of 21) subjects returned for safety and tolerability assessments at days 3, 8, 15 and 29 post-Baseline. 20 of 21 patients returned for final safety assessments at 57 days post-Baseline.
Change in Lesion Size.
-12.86 percentage change in tumour area
Standard Error 5.88

Adverse Events

Treatment With BSCT

Serious events: 1 serious events
Other events: 12 other events
Deaths: 0 deaths

Serious adverse events

Serious adverse events
Measure
Treatment With BSCT
n=21 participants at risk
BSCT (anti-nf-P2X7) 10% Ointment topically applied twice daily for 28 consecutive days
Cardiac disorders
Acute myocardial infarction
4.8%
1/21 • Number of events 1 • 8 weeks (28 day treatment period and 28 day follow up period).
All (21 of 21) subjects returned for safety and tolerability assessments at days 3, 8, 15 and 29 post-Baseline. 20 of 21 patients returned for final safety assessments was at 57 days post-Baseline.

Other adverse events

Other adverse events
Measure
Treatment With BSCT
n=21 participants at risk
BSCT (anti-nf-P2X7) 10% Ointment topically applied twice daily for 28 consecutive days
General disorders
Application site dryness
33.3%
7/21 • Number of events 7 • 8 weeks (28 day treatment period and 28 day follow up period).
All (21 of 21) subjects returned for safety and tolerability assessments at days 3, 8, 15 and 29 post-Baseline. 20 of 21 patients returned for final safety assessments was at 57 days post-Baseline.
General disorders
Application site erythema
47.6%
10/21 • Number of events 10 • 8 weeks (28 day treatment period and 28 day follow up period).
All (21 of 21) subjects returned for safety and tolerability assessments at days 3, 8, 15 and 29 post-Baseline. 20 of 21 patients returned for final safety assessments was at 57 days post-Baseline.
General disorders
Application site exfoliation
23.8%
5/21 • Number of events 5 • 8 weeks (28 day treatment period and 28 day follow up period).
All (21 of 21) subjects returned for safety and tolerability assessments at days 3, 8, 15 and 29 post-Baseline. 20 of 21 patients returned for final safety assessments was at 57 days post-Baseline.
General disorders
Application site pain
14.3%
3/21 • Number of events 3 • 8 weeks (28 day treatment period and 28 day follow up period).
All (21 of 21) subjects returned for safety and tolerability assessments at days 3, 8, 15 and 29 post-Baseline. 20 of 21 patients returned for final safety assessments was at 57 days post-Baseline.
General disorders
Application site pruritus
23.8%
5/21 • Number of events 5 • 8 weeks (28 day treatment period and 28 day follow up period).
All (21 of 21) subjects returned for safety and tolerability assessments at days 3, 8, 15 and 29 post-Baseline. 20 of 21 patients returned for final safety assessments was at 57 days post-Baseline.
Infections and infestations
Sinusitis
4.8%
1/21 • Number of events 1 • 8 weeks (28 day treatment period and 28 day follow up period).
All (21 of 21) subjects returned for safety and tolerability assessments at days 3, 8, 15 and 29 post-Baseline. 20 of 21 patients returned for final safety assessments was at 57 days post-Baseline.
Gastrointestinal disorders
Diarrhoea
4.8%
1/21 • Number of events 1 • 8 weeks (28 day treatment period and 28 day follow up period).
All (21 of 21) subjects returned for safety and tolerability assessments at days 3, 8, 15 and 29 post-Baseline. 20 of 21 patients returned for final safety assessments was at 57 days post-Baseline.

Additional Information

Dr Shaun McNulty

Biosceptre UK

Phone: 01223496092

Results disclosure agreements

  • Principal investigator is a sponsor employee
  • Publication restrictions are in place