Causes of Fever in Bangladeshi Patients

Summary

Background:

The clinical features and prevalence of tropical rickettsial illnesses such as murine and scrub typhus in Bangladesh are unknown. Following testing for malaria, patients with undifferentiated fever are frequently treated empirically for typhoid or diagnosed clinically with a viral fever. Since murine and scrub typhus are common causes of fever in other countries in the region, it is likely they are prevalent in Bangladesh. Murine and scrub typhus may be treated cheaply and effectively with doxycycline.

Primary aim:

\- Describe the clinical features of scrub and murine typhus in Bangladeshi patients

Secondary aims:

* Assess the proportion of patients screened for malaria having rickettsial illnesses
* Understand the pathophysiology of severe scrub typhus and murine typhus
* Prospective evaluation of rapid diagnostic tests for scrub and murine typhus

Methods:

Scrub typhus and murine typhus rapid tests will be introduced to CMCH in conjunction with existing malaria testing facilities. Consenting febrile adult patients who have had malaria and typhus rapid tests and meeting the entry criteria will be enrolled. Samples will be saved for serology and real time polymerase chain reaction (PCR) testing for O. tsutsugamushi and Rickettsia spp. A thorough history and examination will be undertaken. Hemodynamic status will be assessed by ultrasound upon enrolment. Patients will be followed up for outcome and a second sample will be taken for convalescent serological testing on day 14 where possible.

Analysis The proportion of patients screened for malaria with an acute febrile illness due to scrub typhus and murine typhus will be calculated. The clinical features of scrub and murine typhus, malaria and patients negative for these conditions will be compared. Healthy subject samples will be used to provide normal ranges. The sensitivity and specificity of the rapid tests will be assessed as compared to the gold standard of PCR and serology combined.

Conditions

• Rickettsial Disease

Interventions

OTHER

Observation

Sponsors & Collaborators

• Chittagong Medical College and Hospital

  collaborator OTHER

• University of Amsterdam

  collaborator OTHER

• Menzies School of Health Research

  collaborator OTHER

• Mahidol Oxford Tropical Medicine Research Unit

  collaborator OTHER

• University of Oxford

  lead OTHER

Principal Investigators

• Amir Hossain, Professor Md · Chittagong Medical College and Hospital

Eligibility

Min Age

12 Years

Sex

ALL

Healthy Volunteers

Yes

Timeline & Regulatory

Start

2014-08-31

Primary Completion

2015-09-30

Completion

2015-09-30

Countries

• Bangladesh

Study Locations