Trial Outcomes & Findings for Comparing the Effect of Whey and Soy Protein on the Absorption of Pomegranate Polyphenols in the Human Body (NCT NCT02233478)

NCT ID: NCT02233478

Last Updated: 2019-09-19

Results Overview

Blood samples were collected at baseline, 0.5, 1, 2, 3, 4, 6 and 24 h after ingestion of pomegranate juice (PJ) alone, or PJ mixed with soy protein, or PJ mixed with soybean flour. Plasma concentration of ellagic acid at each time point was determined to create a pharmacokinetic parameter area under the curve.

Recruitment status

COMPLETED

Study phase

NA

Target enrollment

18 participants

Primary outcome timeframe

Baseline, 0.5, 1, 2, 3, 4, 6 and 24 hr after 1 dosing of each intervention during the 3-week period

Results posted on

2019-09-19

Participant Flow

The enrollment period was 08/01/2014 - 01/31/2015. Enrollment took place in a private setting in the clinic located at the site.

After a 4-day washout period, participants received pomegranate juice (PJ) alone, PJ mixed with soy protein, or PJ mixed with soybean flour in random sequence with a 7-day washout in between.

Participant milestones

Participant milestones
Measure
PJ First, Then PJ With Soybean Flour, Then PJ With Soy Protein
Participants consumed PJ alone, then PJ+ soybean flour, then PJ + soy protein with PJ dosing 236.5 mL, soy protein 20 g and soybean flour 20 g
PJ First, Then PJ With Soy Protein, Then PJ With Soybean Flour
Participants consumed PJ alone, then PJ+ soy protein, then PJ + soybean flour with PJ dosing 236.5 mL, soy protein 20 g and soybean flour 20 g
PJ With Soybean Flour First, Then PJ, Then PJ With Soy Protein
Participants consumed PJ+soybean flour, then PJ alone, then PJ+soy protein with PJ dosing 236.5 mL, soy protein 20 g and soybean flour 20 g
PJ With Soybean Flour First, Then PJ With Soy Protein, Then PJ
Participants consumed PJ+soybean flour, then PJ+soy protein, then PJ alone with PJ dosing 236.5 mL, soy protein 20 g and soybean flour 20 g
PJ With Soy Protein First, Then PJ With Soybean Flour, Then PJ
Participants consumed PJ+soy protein, then PJ+soybean flour, then PJ alone with PJ dosing 236.5 mL, soy protein 20 g and soybean flour 20 g
PJ With Soy Protein First, Then PJ, Then PJ With Soybean Flour
Participants consumed PJ+soy protein, then PJ alone, then PJ+soybean flour with PJ dosing 236.5 mL, soy protein 20 g and soybean flour 20 g
First Intervention (1 Day)
STARTED
3
3
3
3
3
3
First Intervention (1 Day)
COMPLETED
3
3
3
3
3
3
First Intervention (1 Day)
NOT COMPLETED
0
0
0
0
0
0
Washout (7 Days)
STARTED
3
3
3
3
3
3
Washout (7 Days)
COMPLETED
3
3
3
3
3
3
Washout (7 Days)
NOT COMPLETED
0
0
0
0
0
0
Second Intervention (1 Day)
STARTED
3
3
3
3
3
3
Second Intervention (1 Day)
COMPLETED
3
3
3
3
3
3
Second Intervention (1 Day)
NOT COMPLETED
0
0
0
0
0
0
Third Intervention (1 Day)
STARTED
3
3
3
3
3
3
Third Intervention (1 Day)
COMPLETED
3
3
3
3
3
3
Third Intervention (1 Day)
NOT COMPLETED
0
0
0
0
0
0

Reasons for withdrawal

Withdrawal data not reported

Baseline Characteristics

Comparing the Effect of Whey and Soy Protein on the Absorption of Pomegranate Polyphenols in the Human Body

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
All Study Participants
n=18 Participants
Participants took pomegranate juice (PJ, 236.5 mL or 8 fl.oz) alone, or PJ (236.5 mL) mixed with 20 g of soy protein, or PJ (236.5 mL) mixed with 20 g of soybean flour in random order.
Age, Categorical
<=18 years
0 Participants
n=99 Participants
Age, Categorical
Between 18 and 65 years
18 Participants
n=99 Participants
Age, Categorical
>=65 years
0 Participants
n=99 Participants
Age, Continuous
31.1 years
STANDARD_DEVIATION 7.0 • n=99 Participants
Sex: Female, Male
Female
9 Participants
n=99 Participants
Sex: Female, Male
Male
9 Participants
n=99 Participants
Race (NIH/OMB)
American Indian or Alaska Native
0 Participants
n=99 Participants
Race (NIH/OMB)
Asian
6 Participants
n=99 Participants
Race (NIH/OMB)
Native Hawaiian or Other Pacific Islander
0 Participants
n=99 Participants
Race (NIH/OMB)
Black or African American
6 Participants
n=99 Participants
Race (NIH/OMB)
White
6 Participants
n=99 Participants
Race (NIH/OMB)
More than one race
0 Participants
n=99 Participants
Race (NIH/OMB)
Unknown or Not Reported
0 Participants
n=99 Participants
Region of Enrollment
United States
18 participants
n=99 Participants
BMI
26.3 kg/m^2
STANDARD_DEVIATION 6.0 • n=99 Participants

PRIMARY outcome

Timeframe: Baseline, 0.5, 1, 2, 3, 4, 6 and 24 hr after 1 dosing of each intervention during the 3-week period

Population: All participants for whom plasma ellagic acid was determined at baseline, 0.5, 1, 2, 3, 4, 6 and 24 hr after 1 dosing of PJ, or PJ with soy protein, or PJ with soybean flour

Blood samples were collected at baseline, 0.5, 1, 2, 3, 4, 6 and 24 h after ingestion of pomegranate juice (PJ) alone, or PJ mixed with soy protein, or PJ mixed with soybean flour. Plasma concentration of ellagic acid at each time point was determined to create a pharmacokinetic parameter area under the curve.

Outcome measures

Outcome measures
Measure
All Study Participants Consuming PJ
n=18 Participants
Participants consumed PJ (236.5 mL) only
All Study Participants Comsuming PJ With Soy Protein
n=18 Participants
Participants consumed PJ (236.5 mL) mixed with soy protein isolate (20 g)
All Study Participants Comsuming PJ With Soybean Flour
Participants consumed PJ (236.5 mL) with soybean flour protein (20 g)
Plasma Ellagic Acid Concentration 0 to 24 Hours Post-dose Area Under the Curve
0.074 micromol*h/L
Standard Deviation 0.029
0.075 micromol*h/L
Standard Deviation 0.028

Adverse Events

Study Participants Consuming PJ Alone

Serious events: 0 serious events
Other events: 0 other events
Deaths: 0 deaths

Study Participants Consuming PJ With Soy Protein

Serious events: 0 serious events
Other events: 0 other events
Deaths: 0 deaths

Study Participants Consuming PJ With Soybean Flour

Serious events: 0 serious events
Other events: 0 other events
Deaths: 0 deaths

Serious adverse events

Adverse event data not reported

Other adverse events

Adverse event data not reported

Additional Information

Zhaoping Li, Professor

Center for Human Nutrition, Department of Medicine, UCLA David Geffen School of Medicine

Phone: 310-206-1987

Results disclosure agreements

  • Principal investigator is a sponsor employee
  • Publication restrictions are in place