Trial Outcomes & Findings for Alendronate to Prevent Loss of Bronchoprotection in Asthma (NCT NCT02230332)

NCT ID: NCT02230332

Last Updated: 2018-01-12

Results Overview

Following administration of Salmeterol, the concentration of Methacholine required to produce a 20% drop in FEV1 - measured in mg/ml and reported on log base 2 scale.

Recruitment status

COMPLETED

Study phase

PHASE2/PHASE3

Target enrollment

78 participants

Primary outcome timeframe

8 weeks after randomization

Results posted on

2018-01-12

Participant Flow

Eligible participants were recruited from the community between January 2015 and May 2016 at 9 U.S. sites from the NHLBI AsthmaNet research network.

Participants with persistent asthma were first treated with fluticasone propionate 250mcg twice daily for 2 weeks during the run-in. Most of the participants who were not randomized did not meet the Salmeterol Protected Methacholine Challenge inclusion criterion.

Participant milestones

Participant milestones
Measure
Alendronate
Alendronate in 10mg capsules taken once daily Alendronate
Placebo
Placebo capsule taken once daily Placebo
Overall Study
STARTED
38
40
Overall Study
COMPLETED
34
39
Overall Study
NOT COMPLETED
4
1

Reasons for withdrawal

Reasons for withdrawal
Measure
Alendronate
Alendronate in 10mg capsules taken once daily Alendronate
Placebo
Placebo capsule taken once daily Placebo
Overall Study
Adverse Event
2
1
Overall Study
Lost to Follow-up
1
0
Overall Study
Protocol Violation
1
0

Baseline Characteristics

Alendronate to Prevent Loss of Bronchoprotection in Asthma

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
Alendronate
n=38 Participants
Alendronate in 10mg capsules taken once daily Alendronate
Placebo
n=40 Participants
Placebo capsule taken once daily Placebo
Total
n=78 Participants
Total of all reporting groups
Age, Continuous
38.3 years
STANDARD_DEVIATION 13.1 • n=39 Participants
39.3 years
STANDARD_DEVIATION 12.3 • n=41 Participants
38.8 years
STANDARD_DEVIATION 12.6 • n=35 Participants
Sex: Female, Male
Female
20 Participants
n=39 Participants
27 Participants
n=41 Participants
47 Participants
n=35 Participants
Sex: Female, Male
Male
18 Participants
n=39 Participants
13 Participants
n=41 Participants
31 Participants
n=35 Participants
Race/Ethnicity, Customized
White
24 Participants
n=39 Participants
21 Participants
n=41 Participants
45 Participants
n=35 Participants
Race/Ethnicity, Customized
Black
10 Participants
n=39 Participants
13 Participants
n=41 Participants
23 Participants
n=35 Participants
Race/Ethnicity, Customized
Asian/Pacific Islander
1 Participants
n=39 Participants
3 Participants
n=41 Participants
4 Participants
n=35 Participants
Race/Ethnicity, Customized
Hispanic or Latio
3 Participants
n=39 Participants
3 Participants
n=41 Participants
6 Participants
n=35 Participants
Region of Enrollment
United States
38 participants
n=39 Participants
40 participants
n=41 Participants
78 participants
n=35 Participants
Asthma Control Test score
21 units on a scale
n=39 Participants
21 units on a scale
n=41 Participants
21 units on a scale
n=35 Participants
FEV1 - percent of predicted
81.3 percent of predicted
STANDARD_DEVIATION 14.9 • n=39 Participants
83.1 percent of predicted
STANDARD_DEVIATION 14.0 • n=41 Participants
82.2 percent of predicted
STANDARD_DEVIATION 14.4 • n=35 Participants
Salmeterol Protected Methcholine PC20
5.4 mg/ml
STANDARD_DEVIATION 0.8 • n=39 Participants
3.7 mg/ml
STANDARD_DEVIATION 1 • n=41 Participants
4.5 mg/ml
STANDARD_DEVIATION 0.9 • n=35 Participants

PRIMARY outcome

Timeframe: 8 weeks after randomization

Following administration of Salmeterol, the concentration of Methacholine required to produce a 20% drop in FEV1 - measured in mg/ml and reported on log base 2 scale.

Outcome measures

Outcome measures
Measure
Alendronate
n=34 Participants
Alendronate in 10mg capsules taken once daily Alendronate
Placebo
n=39 Participants
Placebo capsule taken once daily Placebo
Salmeterol Protected Methacholine Challenge PC20
1.8 mg/ml on log base 2 scale
Interval 1.1 to 2.5
1.7 mg/ml on log base 2 scale
Interval 1.1 to 2.3

SECONDARY outcome

Timeframe: 8 weeks after randomization

Outcome measures

Outcome measures
Measure
Alendronate
n=34 Participants
Alendronate in 10mg capsules taken once daily Alendronate
Placebo
n=39 Participants
Placebo capsule taken once daily Placebo
Peripheral Blood Mononuclear Cell ADRB2 Cell Surface Density
1680 number of receptors per cell
Interval 1359.0 to 2076.0
1863 number of receptors per cell
Interval 1473.0 to 2358.0

SECONDARY outcome

Timeframe: 8 weeks after randomization

Peripheral blood mononuclear cells cAMP concentrations measured using isoproterenol (ISO) as a beta-2 adrenergic receptor agonist, and using phosphate buffered saline (PBS) as a positive control. The outcome is expressed as the ratio of cAMP concentration using ISO relative to cAMP concentration using PBS.

Outcome measures

Outcome measures
Measure
Alendronate
n=34 Participants
Alendronate in 10mg capsules taken once daily Alendronate
Placebo
n=39 Participants
Placebo capsule taken once daily Placebo
Beta-2 Adrenergic Receptor Agonist-induced cAMP Production
5.9 ratio
Interval 4.7 to 7.5
5.9 ratio
Interval 4.9 to 7.2

OTHER_PRE_SPECIFIED outcome

Timeframe: 8 weeks after randomization

Salivary Alpha Amylase (sAA) levels from saliva samples obtained through passive drooling, before and 1 hour after Salmeterol administration. The outcome is expressed as the ratio of the Post-Salmeterol to the Pre-Salmeterol sAA levels.

Outcome measures

Outcome measures
Measure
Alendronate
n=34 Participants
Alendronate in 10mg capsules taken once daily Alendronate
Placebo
n=39 Participants
Placebo capsule taken once daily Placebo
Salivary Alpha Amylase Ratio (Post-Salmeterol / Pre-Salmeterol)
1.6 ratio
Interval 1.4 to 1.9
1.6 ratio
Interval 1.3 to 1.9

OTHER_PRE_SPECIFIED outcome

Timeframe: 8 weeks after randomization

Asthma Control Test : Score calculated as the sum total of a 5-item questionnaire. Each item ranges from 1 (poor control) to 5 (good control) so that the range of the total score is 5 to 25. Scores below 20 indicate that asthma is not well controlled.

Outcome measures

Outcome measures
Measure
Alendronate
n=34 Participants
Alendronate in 10mg capsules taken once daily Alendronate
Placebo
n=39 Participants
Placebo capsule taken once daily Placebo
Asthma Control Test (ACT)
22.2 units on a scale
Interval 21.2 to 23.2
22.2 units on a scale
Interval 21.4 to 23.1

OTHER_PRE_SPECIFIED outcome

Timeframe: 8 weeks after randomization

Outcome measures

Outcome measures
Measure
Alendronate
n=34 Participants
Alendronate in 10mg capsules taken once daily Alendronate
Placebo
n=39 Participants
Placebo capsule taken once daily Placebo
Fractional Exhaled Nitrix Oxide
16.8 parts per billion
Interval 12.9 to 21.9
15.4 parts per billion
Interval 11.7 to 20.1

Adverse Events

Alendronate

Serious events: 0 serious events
Other events: 9 other events
Deaths: 0 deaths

Placebo

Serious events: 2 serious events
Other events: 13 other events
Deaths: 0 deaths

Serious adverse events

Serious adverse events
Measure
Alendronate
n=38 participants at risk
Alendronate in 10mg capsules taken once daily Alendronate
Placebo
n=40 participants at risk
Placebo capsule taken once daily Placebo
Respiratory, thoracic and mediastinal disorders
Viral Pneumonia
0.00%
0/38 • 8 weeks
2.5%
1/40 • Number of events 1 • 8 weeks
Gastrointestinal disorders
GI Bleed
0.00%
0/38 • 8 weeks
2.5%
1/40 • Number of events 1 • 8 weeks

Other adverse events

Other adverse events
Measure
Alendronate
n=38 participants at risk
Alendronate in 10mg capsules taken once daily Alendronate
Placebo
n=40 participants at risk
Placebo capsule taken once daily Placebo
Gastrointestinal disorders
Esophageal reflux
5.3%
2/38 • Number of events 2 • 8 weeks
0.00%
0/40 • 8 weeks
Respiratory, thoracic and mediastinal disorders
Acute nasopharyngitis
7.9%
3/38 • Number of events 4 • 8 weeks
5.0%
2/40 • Number of events 2 • 8 weeks
Respiratory, thoracic and mediastinal disorders
Acute sinusitis
7.9%
3/38 • Number of events 3 • 8 weeks
0.00%
0/40 • 8 weeks
Respiratory, thoracic and mediastinal disorders
Acue Upper Respiratory Infection
0.00%
0/38 • 8 weeks
10.0%
4/40 • Number of events 4 • 8 weeks
Respiratory, thoracic and mediastinal disorders
asthma exacerbation
0.00%
0/38 • 8 weeks
5.0%
2/40 • Number of events 3 • 8 weeks
Infections and infestations
thrush
0.00%
0/38 • 8 weeks
5.0%
2/40 • Number of events 2 • 8 weeks
General disorders
headache
5.3%
2/38 • Number of events 2 • 8 weeks
10.0%
4/40 • Number of events 5 • 8 weeks
General disorders
Heartburn
5.3%
2/38 • Number of events 2 • 8 weeks
0.00%
0/40 • 8 weeks
General disorders
Nausea alone
0.00%
0/38 • 8 weeks
7.5%
3/40 • Number of events 5 • 8 weeks

Additional Information

David Mauger, PhD

Penn State University Dept of Public Health Sciences

Phone: 717.531.3584

Results disclosure agreements

  • Principal investigator is a sponsor employee
  • Publication restrictions are in place