Trial Outcomes & Findings for Healthy Aging Through Functional Food (NCT NCT02095873)

NCT ID: NCT02095873

Last Updated: 2017-03-06

Results Overview

A standard 75 g glucose oGTT will be performed, as routinely used in clinical practice. Participants will be instructed to eat carbohydrate rich diet (\> 150 g/day) for at least three days before the test, followed by an overnight fast. Participants will be instructed to have comparable macronutrient composition of the dinner before the respective study days in the metabolic unit. During the oGTT both capillary and venous blood samples will be collected after 0, 15, 30, 60, 90 and 120 min. To minimize the inconvenience of repeated blood tests during the oGTT, a venous cannula will be inserted, under sterile conditions, prior to the test, for blood sampling.

Recruitment status

COMPLETED

Study phase

PHASE1/PHASE2

Target enrollment

32 participants

Primary outcome timeframe

Week 0 and Week 8 (first intervention); Week 14 and Week 22 (second intervention)

Results posted on

2017-03-06

Participant Flow

Participant milestones

Participant milestones
Measure
Glyoxalase 1 Inducer First, Then Placebo
Glyoxalase 1 inducer (capsule, 90 mg trans-resveratrol \& 120 mg hesperetin combination) once daily, 8 weeks; then Placebo (excipient: Mannitol, 108 mg) once daily, 8 weeks.
Placebo Then Glyoxalase 1 Inducer
Placebo (excipient: Mannitol, 108 mg), once daily, 8 weeks; then Glyoxalase 1 inducer (capsule, 90 mg trans-resveratrol \& 120 mg hesperetin combination) once daily, 8 weeks.
First Intervention (8 Weeks)
STARTED
16
16
First Intervention (8 Weeks)
COMPLETED
15
16
First Intervention (8 Weeks)
NOT COMPLETED
1
0
Second Intervention (8 Weeks)
STARTED
15
16
Second Intervention (8 Weeks)
COMPLETED
13
16
Second Intervention (8 Weeks)
NOT COMPLETED
2
0

Reasons for withdrawal

Reasons for withdrawal
Measure
Glyoxalase 1 Inducer First, Then Placebo
Glyoxalase 1 inducer (capsule, 90 mg trans-resveratrol \& 120 mg hesperetin combination) once daily, 8 weeks; then Placebo (excipient: Mannitol, 108 mg) once daily, 8 weeks.
Placebo Then Glyoxalase 1 Inducer
Placebo (excipient: Mannitol, 108 mg), once daily, 8 weeks; then Glyoxalase 1 inducer (capsule, 90 mg trans-resveratrol \& 120 mg hesperetin combination) once daily, 8 weeks.
First Intervention (8 Weeks)
Withdrawal by Subject
1
0
Second Intervention (8 Weeks)
Withdrawal by Subject
1
0
Second Intervention (8 Weeks)
Protocol Violation
1
0

Baseline Characteristics

Healthy Aging Through Functional Food

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
Glyoxalase 1 Inducer Then Placebo
n=15 Participants
Glyoxalase 1 inducer (90 mg trans-resveratrol \& 120 mg hesperetin), once daily, 8 weeks; then Placebo (excipient: 108 mg mannitol), once daily, 8 weeks.
Placebo Then Glyoxalase 1 Inducer
n=17 Participants
Placebo (excipient: 108 mg mannitol), once daily, 8 weeks; then Glyoxalase 1 inducer (90 mg trans-resveratrol \& 120 mg hesperetin), once daily, 8 weeks.
Total
n=32 Participants
Total of all reporting groups
Age, Continuous
45 years
STANDARD_DEVIATION 12 • n=99 Participants
44 years
STANDARD_DEVIATION 13 • n=107 Participants
45 years
STANDARD_DEVIATION 13 • n=206 Participants
Gender
Female
10 Participants
n=99 Participants
12 Participants
n=107 Participants
22 Participants
n=206 Participants
Gender
Male
5 Participants
n=99 Participants
5 Participants
n=107 Participants
10 Participants
n=206 Participants
Region of Enrollment
United Kingdom
15 participants
n=99 Participants
17 participants
n=107 Participants
32 participants
n=206 Participants

PRIMARY outcome

Timeframe: Week 0 and Week 8 (first intervention); Week 14 and Week 22 (second intervention)

Population: Highly overweight/obese (BMI\>27.5 kg/m2) subgroup, based on subject BMI at study entry.

A standard 75 g glucose oGTT will be performed, as routinely used in clinical practice. Participants will be instructed to eat carbohydrate rich diet (\> 150 g/day) for at least three days before the test, followed by an overnight fast. Participants will be instructed to have comparable macronutrient composition of the dinner before the respective study days in the metabolic unit. During the oGTT both capillary and venous blood samples will be collected after 0, 15, 30, 60, 90 and 120 min. To minimize the inconvenience of repeated blood tests during the oGTT, a venous cannula will be inserted, under sterile conditions, prior to the test, for blood sampling.

Outcome measures

Outcome measures
Measure
Glyoxalase 1 Inducer
n=20 Participants
Glyoxalase 1 inducer (90 mg trans-resveratrol \& 120 mg hesperetin), once daily, 8 weeks.
Placebo
n=20 Participants
Placebo (excipient: 108 mg mannitol), capsule, once daily, 8 weeks.
Area Under the Curve for Oral Glucose Tolerance Test (oGGT)
Post-8 weeks treatment
9.9 mM h
Standard Error 0.6
10.6 mM h
Standard Error 0.6
Area Under the Curve for Oral Glucose Tolerance Test (oGGT)
Baseline
10.8 mM h
Standard Error 0.7
11.0 mM h
Standard Error 0.7

SECONDARY outcome

Timeframe: Week 0 and Week 8 (first intervention); Week 14 and Week 22 (second intervention)

Population: All subjects with where baseline and post-8 treatment data were obtained.

After 20 min seated at rest, measurements are made with the subject seated and the left hand at heart level. Nail-fold capillaries in the dorsal skin of the third finger are visualized using a stereo microscope linked to a monochrome digital camera. Capillary density is defined as the number of capillaries per mm2 of nail-fold skin and is computed as the mean of 4 measurements.

Outcome measures

Outcome measures
Measure
Glyoxalase 1 Inducer
n=28 Participants
Glyoxalase 1 inducer (90 mg trans-resveratrol \& 120 mg hesperetin), once daily, 8 weeks.
Placebo
n=24 Participants
Placebo (excipient: 108 mg mannitol), capsule, once daily, 8 weeks.
Finger-fold Capillary Density by Capillaroscopy
Baseline
115 number of capillaries per mm2
Interval 90.0 to 140.0
119 number of capillaries per mm2
Interval 107.0 to 164.0
Finger-fold Capillary Density by Capillaroscopy
Post-8 weeks treatment
125 number of capillaries per mm2
Interval 88.0 to 169.0
128 number of capillaries per mm2
Interval 104.0 to 149.0

SECONDARY outcome

Timeframe: Week 0 and Week 8 (first intervention); Week 14 and Week 22 (second intervention)

Population: All subjects completing the study per protocol

Brachial artery FMD will be assessed. Ultrasound imaging of the brachial artery will be performed. Percent FMD will be calculated using the averaged minimum mean brachial artery diameter at baseline compared to the largest mean values obtained after either release of the forearm occlusion.

Outcome measures

Outcome measures
Measure
Glyoxalase 1 Inducer
n=29 Participants
Glyoxalase 1 inducer (90 mg trans-resveratrol \& 120 mg hesperetin), once daily, 8 weeks.
Placebo
n=29 Participants
Placebo (excipient: 108 mg mannitol), capsule, once daily, 8 weeks.
Flow-mediated Dilatation (FMD)
Post-8 weeks treatment
0.12 percentage of baseline value
Interval 0.06 to 0.31
0.26 percentage of baseline value
Interval 0.07 to 0.47
Flow-mediated Dilatation (FMD)
Baseline
0.17 percentage of baseline value
Interval 0.1 to 0.35
0.18 percentage of baseline value
Interval 0.07 to 0.49

OTHER_PRE_SPECIFIED outcome

Timeframe: Week 0 and Week 8 (first intervention); Week 14 and Week 22 (second intervention)

Population: Subjects for which PWV data were obtained at baseline and post 8-weeks treatment.

Aortal pulse wave velocity is measured by a non-invasive oscillometric device.

Outcome measures

Outcome measures
Measure
Glyoxalase 1 Inducer
n=13 Participants
Glyoxalase 1 inducer (90 mg trans-resveratrol \& 120 mg hesperetin), once daily, 8 weeks.
Placebo
n=12 Participants
Placebo (excipient: 108 mg mannitol), capsule, once daily, 8 weeks.
Aortal Pulse Wave Velocity (aPWV)
Baseline
7.9 m/s
Interval 7.1 to 8.8
8.3 m/s
Interval 7.4 to 9.2
Aortal Pulse Wave Velocity (aPWV)
Post-8 weeks treatment
8.0 m/s
Interval 7.1 to 10.0
8.5 m/s
Interval 7.5 to 9.0

Adverse Events

Glo1-inducer Then Placebo

Serious events: 0 serious events
Other events: 0 other events
Deaths: 0 deaths

Placebo Then Glo1-inducer

Serious events: 0 serious events
Other events: 0 other events
Deaths: 0 deaths

Serious adverse events

Adverse event data not reported

Other adverse events

Adverse event data not reported

Additional Information

Paul J Thornalley

University of Warwick

Phone: +442476968594

Results disclosure agreements

  • Principal investigator is a sponsor employee
  • Publication restrictions are in place