Trial Outcomes & Findings for Evaluation of Drug-drug Interaction Between LCZ696 and Sildenafil in Subjects With Mild to Moderate Hypertension (NCT NCT01601470)

NCT ID: NCT01601470

Last Updated: 2015-10-07

Results Overview

The effect of co-administration of sildenafil on the pharmacokinetics of LCZ696 (analytes of LCZ696: AHU377, LBQ657 and valsartan) was assessed. Blood samples were collected at pre-dose and 0.5, 1, 1.5, 2, 3, 4, 6, 8, 12 hours post-dose from day 1 to day 7, 24 hours post-dose from day 7, and day 8 at 0.5, 1, 1.5, 2, 3, 4, 6, 8, 12 hours post-dose. AUC is a mathematically-derived value from all measurements. AUC is a measure of the area under the curve that is obtained from plotting the plasma concentration by time point. All time-points were used to derive the single PK parameters.

Recruitment status

COMPLETED

Study phase

PHASE2

Target enrollment

28 participants

Primary outcome timeframe

From pre-dose on day 1 until 12 hours post dose on day 8

Results posted on

2015-10-07

Participant Flow

Participant milestones

Participant milestones
Measure
Period 1:Sildenafil;Period 2:LCZ696;Period 3:LCZ696+Sildenafil
During Treatment Period 1, on study Day 1, participants received a single dose of sildenafil followed by a wash out on Day 2. In Period 2 (study Days 3-7), participants received LCZ696 once daily. In Period 3, on study Day 8, participants received LCZ696, co-administered at the same time with a single dose of sildenafil.
Period 1 - Sildenafil
STARTED
28
Period 1 - Sildenafil
PK Analysis Set
28
Period 1 - Sildenafil
Safety Set
28
Period 1 - Sildenafil
COMPLETED
28
Period 1 - Sildenafil
NOT COMPLETED
0
Period 2 - LCZ696
STARTED
28
Period 2 - LCZ696
PK Analysis Set
27
Period 2 - LCZ696
Safety Set
27
Period 2 - LCZ696
COMPLETED
27
Period 2 - LCZ696
NOT COMPLETED
1
Period 3 - LCZ696+Sildenafil
STARTED
27
Period 3 - LCZ696+Sildenafil
PK Analysis Set
27
Period 3 - LCZ696+Sildenafil
Safety Set
27
Period 3 - LCZ696+Sildenafil
COMPLETED
27
Period 3 - LCZ696+Sildenafil
NOT COMPLETED
0

Reasons for withdrawal

Reasons for withdrawal
Measure
Period 1:Sildenafil;Period 2:LCZ696;Period 3:LCZ696+Sildenafil
During Treatment Period 1, on study Day 1, participants received a single dose of sildenafil followed by a wash out on Day 2. In Period 2 (study Days 3-7), participants received LCZ696 once daily. In Period 3, on study Day 8, participants received LCZ696, co-administered at the same time with a single dose of sildenafil.
Period 2 - LCZ696
Physician Decision
1

Baseline Characteristics

Evaluation of Drug-drug Interaction Between LCZ696 and Sildenafil in Subjects With Mild to Moderate Hypertension

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
Period 1:Sildenafil;Period 2:LCZ696;Period 3:LCZ696+Sildenafil
n=28 Participants
During Treatment Period 1, on study Day 1, participants received a single dose of sildenafil followed by a wash out on Day 2. In Period 2 (study Days 3-7), participants received LCZ696 once daily. In Period 3, on study Day 8, participants received LCZ696, co-administered at the same time with a single dose of sildenafil.
Age, Continuous
51.8 Years
STANDARD_DEVIATION 8.5 • n=39 Participants
Sex: Female, Male
Female
0 Participants
n=39 Participants
Sex: Female, Male
Male
28 Participants
n=39 Participants

PRIMARY outcome

Timeframe: From pre-dose on day 1 until 12 hours post dose on day 8

Population: PK Analysis Set (Period 3): This set included participants with evaluable PK data and without any protocol deviations which could impact the PK data.

The effect of co-administration of sildenafil on the pharmacokinetics of LCZ696 (analytes of LCZ696: AHU377, LBQ657 and valsartan) was assessed. Blood samples were collected at pre-dose and 0.5, 1, 1.5, 2, 3, 4, 6, 8, 12 hours post-dose from day 1 to day 7, 24 hours post-dose from day 7, and day 8 at 0.5, 1, 1.5, 2, 3, 4, 6, 8, 12 hours post-dose. AUC is a mathematically-derived value from all measurements. AUC is a measure of the area under the curve that is obtained from plotting the plasma concentration by time point. All time-points were used to derive the single PK parameters.

Outcome measures

Outcome measures
Measure
Period 1:Sildenafil;Period 2:LCZ696;Period 3:LCZ696+Sildenafil
n=27 Participants
During Treatment Period 1, on study Day 1, participants received a single dose of sildenafil followed by a wash out on Day 2. In Period 2 (study Days 3-7), participants received LCZ696 once daily. In Period 3, on study Day 8, participants received LCZ696, co-administered at the same time with a single dose of sildenafil.
Area Under the Plasma Concentration-time Curve From Time Zero to the End of the Dosing Interval (AUCtau) of LCZ696 Analytes
AHU377
3700 h*ng/mL
Standard Deviation 912
Area Under the Plasma Concentration-time Curve From Time Zero to the End of the Dosing Interval (AUCtau) of LCZ696 Analytes
LBQ657
147000 h*ng/mL
Standard Deviation 31000
Area Under the Plasma Concentration-time Curve From Time Zero to the End of the Dosing Interval (AUCtau) of LCZ696 Analytes
valsartan
23600 h*ng/mL
Standard Deviation 9500

PRIMARY outcome

Timeframe: From pre-dose on day 1 until 12 hours post dose on day 8

Population: PK Analysis Set (Period 3): This set included participants with evaluable PK data and without any protocol deviations which could impact the PK data.

The effect of co-administration of sildenafil on the pharmacokinetics of LCZ696 (analytes of LCZ696: AHU377, LBQ657 and valsartan) was assessed. Blood samples were collected at pre-dose and 0.5, 1, 1.5, 2, 3, 4, 6, 8, 12 hours post-dose from day 1 to day 7, 24 hours post-dose from day 7, and day 8 at 0.5, 1, 1.5, 2, 3, 4, 6, 8, 12 hours post-dose. Cmax is a mathematically-derived value from all measurements. Cmax is a measure of the area under the curve that is obtained from plotting the plasma concentration by time point. All time-points were used to derive the single PK parameters.

Outcome measures

Outcome measures
Measure
Period 1:Sildenafil;Period 2:LCZ696;Period 3:LCZ696+Sildenafil
n=27 Participants
During Treatment Period 1, on study Day 1, participants received a single dose of sildenafil followed by a wash out on Day 2. In Period 2 (study Days 3-7), participants received LCZ696 once daily. In Period 3, on study Day 8, participants received LCZ696, co-administered at the same time with a single dose of sildenafil.
Maximum Plasma Concentration Following Drug Administration at Steady State (Cmax,ss) of LCZ696 Analytes (AHU377, LBQ657 and Valsartan)
AHU377
2310 ng/mL
Standard Deviation 1020
Maximum Plasma Concentration Following Drug Administration at Steady State (Cmax,ss) of LCZ696 Analytes (AHU377, LBQ657 and Valsartan)
LBQ696
14000 ng/mL
Standard Deviation 2420
Maximum Plasma Concentration Following Drug Administration at Steady State (Cmax,ss) of LCZ696 Analytes (AHU377, LBQ657 and Valsartan)
valsartan
3350 ng/mL
Standard Deviation 1480

PRIMARY outcome

Timeframe: From pre-dose on day 1 until 12 hours post dose on day 8

Population: PK Analysis Set (Period 3): This set included participants with evaluable PK data and without any protocol deviations which could impact the PK data.

The effect of co-administration of sildenafil on the pharmacokinetics of LCZ696 (analytes of LCZ696: AHU377, LBQ657 and valsartan) was assessed. Blood samples were collected at pre-dose and 0.5, 1, 1.5, 2, 3, 4, 6, 8, 12 hours post-dose from day 1 to day 7, 24 hours post-dose from day 7, day 8 at 0.5, 1, 1.5, 2, 3, 4, 6, 8, 12 hours post-dose. Cmin is a mathematically-derived value from all measurements. Cmin is a measure of the area under the curve that is obtained from plotting the plasma concentration by time point. All time-points were used to derive the single PK parameters.

Outcome measures

Outcome measures
Measure
Period 1:Sildenafil;Period 2:LCZ696;Period 3:LCZ696+Sildenafil
n=27 Participants
During Treatment Period 1, on study Day 1, participants received a single dose of sildenafil followed by a wash out on Day 2. In Period 2 (study Days 3-7), participants received LCZ696 once daily. In Period 3, on study Day 8, participants received LCZ696, co-administered at the same time with a single dose of sildenafil.
Minimum Plasma Concentration Following Drug Administration at Steady State (Cmin,ss) of LCZ696 Analytes (AHU377, LBQ696 and Valsartan)
AHU377
0.0 ng/mL
Standard Deviation 0.0
Minimum Plasma Concentration Following Drug Administration at Steady State (Cmin,ss) of LCZ696 Analytes (AHU377, LBQ696 and Valsartan)
LBQ657
2170 ng/mL
Standard Deviation 733
Minimum Plasma Concentration Following Drug Administration at Steady State (Cmin,ss) of LCZ696 Analytes (AHU377, LBQ696 and Valsartan)
valsartan
197 ng/mL
Standard Deviation 96.9

PRIMARY outcome

Timeframe: From pre-dose on day 1 until 12 hours post dose on day 8

Population: PK Analysis Set (Period 3): This set included participants with evaluable PK data and without any protocol deviations which could impact the PK data.

The effect of co-administration of sildenafil on the pharmacokinetics of LCZ696 (analytes of LCZ696: AHU377, LBQ657 and valsartan) was assessed. Blood samples were collected at pre-dose and 0.5, 1, 1.5, 2, 3, 4, 6, 8, 12 hours post-dose from day 1 to day 7, 24 hours post-dose from day 7, day 8 at 0.5, 1, 1.5, 2, 3, 4, 6, 8, 12 hours post-dose. Tmax is a mathematically-derived value from all measurements. Tmax is a measure of the area under the curve that is obtained from plotting the plasma concentration by time point. All time-points were used to derive the single PK parameters.

Outcome measures

Outcome measures
Measure
Period 1:Sildenafil;Period 2:LCZ696;Period 3:LCZ696+Sildenafil
n=27 Participants
During Treatment Period 1, on study Day 1, participants received a single dose of sildenafil followed by a wash out on Day 2. In Period 2 (study Days 3-7), participants received LCZ696 once daily. In Period 3, on study Day 8, participants received LCZ696, co-administered at the same time with a single dose of sildenafil.
Time to Reach the Maximum Concentration After Drug Administration (Tmax) of LCZ696 Analytes (AHU377, LBQ657 and Valsartan)
AHU377
1.00 hours
Full Range NA • Interval 0.5 to 4.0
Time to Reach the Maximum Concentration After Drug Administration (Tmax) of LCZ696 Analytes (AHU377, LBQ657 and Valsartan)
LBQ657
3.00 hours
Interval 1.5 to 6.0
Time to Reach the Maximum Concentration After Drug Administration (Tmax) of LCZ696 Analytes (AHU377, LBQ657 and Valsartan)
valsartan
2.00 hours
Interval 1.5 to 4.0

PRIMARY outcome

Timeframe: From pre-dose on day 1 until 12 hours post dose on day 8

Population: PK Analysis Set (Period 3): This set included participants with evaluable PK data and without any protocol deviations which could impact the PK data.

The effect of co-administration of LCZ696 on the pharmacokinetics of Sildenafil and N-desmethyl-sildenafil was assessed. Blood samples were collected at pre-dose and 0.5, 1, 1.5, 2, 3, 4, 6, 8, 12 hours post-dose from day 1 to day 7, 24 hours post-dose from day 7, day 8 at 0.5, 1, 1.5, 2, 3, 4, 6, 8, 12 hours post-dose. AUC is a mathematically-derived value from all measurements. AUC is a measure of the area under the curve that is obtained from plotting the plasma concentration by time point. All time-points were used to derive the single PK parameters.

Outcome measures

Outcome measures
Measure
Period 1:Sildenafil;Period 2:LCZ696;Period 3:LCZ696+Sildenafil
n=27 Participants
During Treatment Period 1, on study Day 1, participants received a single dose of sildenafil followed by a wash out on Day 2. In Period 2 (study Days 3-7), participants received LCZ696 once daily. In Period 3, on study Day 8, participants received LCZ696, co-administered at the same time with a single dose of sildenafil.
Area Under the Plasma Concentration-time Curve From Time Zero to Infinity (AUCinf) of Sildenafil and N-desmethyl-sildenafil Analytes
Sildenafil
629 h*ng/mL
Standard Deviation 303
Area Under the Plasma Concentration-time Curve From Time Zero to Infinity (AUCinf) of Sildenafil and N-desmethyl-sildenafil Analytes
N-desmethyl-sildenafil
325 h*ng/mL
Standard Deviation 143

PRIMARY outcome

Timeframe: From pre-dose on day 1 until 12 hours post dose on day 8

Population: PK Analysis Set (Period 3): This set included participants with evaluable PK data and without any protocol deviations which could impact the PK data.

The effect of co-administration of LCZ696 on the pharmacokinetics of Sildenafil and N-desmethyl-sildenafil was assessed. Blood samples were collected at pre-dose and 0.5, 1, 1.5, 2, 3, 4, 6, 8, 12 hours post-dose from day 1 to day 7, 24 hours post-dose from day 7, day 8 at 0.5, 1, 1.5, 2, 3, 4, 6, 8, 12 hours post-dose. AUC is a mathematically-derived value from all measurements. AUC is a measure of the area under the curve that is obtained from plotting the plasma concentration by time point. All time-points were used to derive the single PK parameters.

Outcome measures

Outcome measures
Measure
Period 1:Sildenafil;Period 2:LCZ696;Period 3:LCZ696+Sildenafil
n=27 Participants
During Treatment Period 1, on study Day 1, participants received a single dose of sildenafil followed by a wash out on Day 2. In Period 2 (study Days 3-7), participants received LCZ696 once daily. In Period 3, on study Day 8, participants received LCZ696, co-administered at the same time with a single dose of sildenafil.
Area Under the Plasma Concentration-time Curve From Time Zero to the Time of the Last Quantifiable Concentration (AUClast) of Sildenafil and N-desmethyl-sildenafil Analytes
Sildenafil
612 h*ng/mL
Standard Deviation 297
Area Under the Plasma Concentration-time Curve From Time Zero to the Time of the Last Quantifiable Concentration (AUClast) of Sildenafil and N-desmethyl-sildenafil Analytes
N-desmethyl-sildenafil
305 h*ng/mL
Standard Deviation 133

PRIMARY outcome

Timeframe: From pre-dose on day 1 until 12 hours post dose on day 8

Population: PK Analysis Set (Period 3): This set included participants with evaluable PK data and without any protocol deviations which could impact the PK data.

The effect of co-administration of LCZ696 on the pharmacokinetics of Sildenafil and N-desmethyl-sildenafil was assessed. Blood samples were collected at pre-dose and 0.5, 1, 1.5, 2, 3, 4, 6, 8, 12 hours post-dose from day 1 to day 7, 24 hours post-dose from day 7, day 8 at 0.5, 1, 1.5, 2, 3, 4, 6, 8, 12 hours post-dose. T1/2 is a mathematically-derived value from all measurements. T1/2 is a measure of the area under the curve that is obtained from plotting the plasma concentration by time point. All time-points were used to derive the single PK parameters.

Outcome measures

Outcome measures
Measure
Period 1:Sildenafil;Period 2:LCZ696;Period 3:LCZ696+Sildenafil
n=27 Participants
During Treatment Period 1, on study Day 1, participants received a single dose of sildenafil followed by a wash out on Day 2. In Period 2 (study Days 3-7), participants received LCZ696 once daily. In Period 3, on study Day 8, participants received LCZ696, co-administered at the same time with a single dose of sildenafil.
Terminal Elimination Half-life (T1/2) of Sildenafil and N-desmethyl-sildenafil Analytes
Sildenafil
3.84 hours
Standard Deviation 1.11
Terminal Elimination Half-life (T1/2) of Sildenafil and N-desmethyl-sildenafil Analytes
N-desmethyl-sildenafil
6.20 hours
Standard Deviation 1.90

PRIMARY outcome

Timeframe: From pre-dose on day 1 until 12 hours post dose on day 8

Population: PK Analysis Set (Period 3): This set included participants with evaluable PK data and without any protocol deviations which could impact the PK data.

The effect of co-administration of LCZ696 on the pharmacokinetics of Sildenafil and N-desmethyl-sildenafil was assessed. Blood samples were collected at pre-dose and 0.5, 1, 1.5, 2, 3, 4, 6, 8, 12 hours post-dose from day 1 to day 7, 24 hours post-dose from day 7, day 8 at 0.5, 1, 1.5, 2, 3, 4, 6, 8, 12 hours post-dose. All time-points were used to mathematically derive the single PK parameter.

Outcome measures

Outcome measures
Measure
Period 1:Sildenafil;Period 2:LCZ696;Period 3:LCZ696+Sildenafil
n=27 Participants
During Treatment Period 1, on study Day 1, participants received a single dose of sildenafil followed by a wash out on Day 2. In Period 2 (study Days 3-7), participants received LCZ696 once daily. In Period 3, on study Day 8, participants received LCZ696, co-administered at the same time with a single dose of sildenafil.
Maximum Plasma Concentration Following Drug Administration (Cmax) of Sildenafil and N-desmethyl-sildenafil Analytes
Sildenafil
189 ng/mL
Standard Deviation 99.6
Maximum Plasma Concentration Following Drug Administration (Cmax) of Sildenafil and N-desmethyl-sildenafil Analytes
N-desmethyl-sildenafil
84.6 ng/mL
Standard Deviation 39.3

PRIMARY outcome

Timeframe: From pre-dose on day 1 until 12 hours post dose on day 8

Population: PK Analysis Set (Period 3): This set included participants with evaluable PK data and without any protocol deviations which could impact the PK data.

The effect of co-administration of LCZ696 on the pharmacokinetics of Sildenafil and N-desmethyl-sildenafil will be assessed. 8pre-dose and 0.5, 1, 1.5, 2, 3, 4, 6, 8, 12 hours post-dose from day 1 to day 7, 24 hours post-dose from day 7, day 8 at 0.5, 1, 1.5, 2, 3, 4, 6, 8, 12 hours post-dose. All time-points were used to mathematically derive the single PK parameter.

Outcome measures

Outcome measures
Measure
Period 1:Sildenafil;Period 2:LCZ696;Period 3:LCZ696+Sildenafil
n=27 Participants
During Treatment Period 1, on study Day 1, participants received a single dose of sildenafil followed by a wash out on Day 2. In Period 2 (study Days 3-7), participants received LCZ696 once daily. In Period 3, on study Day 8, participants received LCZ696, co-administered at the same time with a single dose of sildenafil.
Time to Reach the Maximum Concentration After Drug Administration (Tmax) of Sildenafil and N-desmethyl-sildenafil Analytes
Sildenafil
1.00 hours
Full Range NA • Interval 0.5 to 3.0
Time to Reach the Maximum Concentration After Drug Administration (Tmax) of Sildenafil and N-desmethyl-sildenafil Analytes
N-desmethyl-sildenafil
1.00 hours
Full Range NA • Interval 0.5 to 3.0

SECONDARY outcome

Timeframe: From the screening visit until 30 days past the final study assessment

Population: Safety Analysis Set: This set included participants who received at least one dose of study drug.

Number of patients with adverse events, serious adverse events and death

Outcome measures

Outcome measures
Measure
Period 1:Sildenafil;Period 2:LCZ696;Period 3:LCZ696+Sildenafil
n=28 Participants
During Treatment Period 1, on study Day 1, participants received a single dose of sildenafil followed by a wash out on Day 2. In Period 2 (study Days 3-7), participants received LCZ696 once daily. In Period 3, on study Day 8, participants received LCZ696, co-administered at the same time with a single dose of sildenafil.
Adverse Events, Serious Adverse Events and Deaths Were Monitored From Screening to End of Study
Adverse Events (Serious and non-serious)
17 Participants
Adverse Events, Serious Adverse Events and Deaths Were Monitored From Screening to End of Study
Serious Adverse Events
0 Participants
Adverse Events, Serious Adverse Events and Deaths Were Monitored From Screening to End of Study
Deaths
0 Participants

Adverse Events

Period 1: Sildenafil

Serious events: 0 serious events
Other events: 7 other events
Deaths: 0 deaths

Period 2: LCZ696

Serious events: 0 serious events
Other events: 5 other events
Deaths: 0 deaths

Period 3: LCZ696 + Sildenafil

Serious events: 0 serious events
Other events: 6 other events
Deaths: 0 deaths

Serious adverse events

Adverse event data not reported

Other adverse events

Other adverse events
Measure
Period 1: Sildenafil
n=28 participants at risk
During Treatment Period 1, on study Day 1, participants received a single dose of sildenafil followed by wash out on Day 2.
Period 2: LCZ696
n=27 participants at risk
In Period 2 (study Days 3-7), participants received LCZ696 once daily.
Period 3: LCZ696 + Sildenafil
n=27 participants at risk
In Period 3, on study Day 8, participants received LCZ696 , co-administered at the same time with a single dose of sildenafil.
Eye disorders
EYE IRRITATION
7.1%
2/28
0.00%
0/27
0.00%
0/27
Gastrointestinal disorders
DIARRHOEA
0.00%
0/28
11.1%
3/27
3.7%
1/27
Nervous system disorders
DIZZINESS
0.00%
0/28
7.4%
2/27
11.1%
3/27
Nervous system disorders
HEADACHE
10.7%
3/28
0.00%
0/27
7.4%
2/27
Renal and urinary disorders
POLLAKIURIA
0.00%
0/28
7.4%
2/27
0.00%
0/27
Respiratory, thoracic and mediastinal disorders
NASAL CONGESTION
7.1%
2/28
0.00%
0/27
0.00%
0/27

Additional Information

Study Director

Novartis Pharmaceuticals

Phone: 862-778-8300

Results disclosure agreements

  • Principal investigator is a sponsor employee The terms and conditions of Novartis' agreements with its investigators may vary. However, Novartis does not prohibit any investigator from publishing. Any publications from a single-site are postponed until the publication of the pooled data (i.e., data from all sites) in the clinical trial or disclosure of trial results in their entirety.
  • Publication restrictions are in place

Restriction type: OTHER