Trial Outcomes & Findings for Efficacy of Brooke Bond Black Tea Extract on Flow Mediated Dilation in Indian Males (NCT NCT01561300)

NCT ID: NCT01561300

Last Updated: 2017-04-05

Results Overview

Change in flow mediated dilation (FMD) due to tea consumption when compared to control. FMD measurement included the following steps: * 1 minute base scan to measure the baseline diameter of artery (baseline) * 5 minutes of forearm occlusion at 300±30 mmHg, just below the elbow 2-5 cm from antecubital crease * 4 minutes FMD scan, which started immediately after release of the occlusion (reactive hyperaemia stage) FMD was calculated as maximal percentage change in diameter of the artery above the baseline value after the release of the occlusion

Recruitment status

COMPLETED

Study phase

NA

Target enrollment

30 participants

Primary outcome timeframe

Baseline day 1 to 2 hours post consumption on day 8.

Results posted on

2017-04-05

Participant Flow

Forty five subjects were invited for screening. Two subjects did not come for the screening, three subjects were not willing to participate and three subjects did not fulfil the inclusion criteria. Of the 37 subjects that fulfilled all inclusion an exclusion criteria 30 were invited to participate.

Thirty subjects meeting all criteria were randomised over the two arms

Participant milestones

Participant milestones
Measure
Control, Then Washout, Then Tea
Subjects first received Control for one week with Flow Mediated Dilation (FMD) measurements on day 1 and day 8. After a washout of one week (day 8-15) they received Tea for one week with FMD measurements at day 16 and day 22. All FMD measurements were done in a fasted state just before test product intake and exactly 2 hours after test product intake.
Tea, Then Wash Out, Then Control
Subjects first received Tea for one week with with Flow Mediated Dilation (FMD) measurements on day 1 and day 8. After a washout of one week (day 8-15) they received Control for one week with FMD measurements at day 16 and day 22. All FMD measurements were done in a fasted state just before test product intake and exactly 2 hours after test product intake.
Run in on Placebo
STARTED
15
15
Run in on Placebo
COMPLETED
14
15
Run in on Placebo
NOT COMPLETED
1
0
First Intervention
STARTED
14
15
First Intervention
COMPLETED
14
15
First Intervention
NOT COMPLETED
0
0
Washout on Placebo
STARTED
14
15
Washout on Placebo
COMPLETED
14
15
Washout on Placebo
NOT COMPLETED
0
0
Second Intervention
STARTED
14
15
Second Intervention
COMPLETED
14
15
Second Intervention
NOT COMPLETED
0
0

Reasons for withdrawal

Reasons for withdrawal
Measure
Control, Then Washout, Then Tea
Subjects first received Control for one week with Flow Mediated Dilation (FMD) measurements on day 1 and day 8. After a washout of one week (day 8-15) they received Tea for one week with FMD measurements at day 16 and day 22. All FMD measurements were done in a fasted state just before test product intake and exactly 2 hours after test product intake.
Tea, Then Wash Out, Then Control
Subjects first received Tea for one week with with Flow Mediated Dilation (FMD) measurements on day 1 and day 8. After a washout of one week (day 8-15) they received Control for one week with FMD measurements at day 16 and day 22. All FMD measurements were done in a fasted state just before test product intake and exactly 2 hours after test product intake.
Run in on Placebo
Adverse Event
1
0

Baseline Characteristics

Efficacy of Brooke Bond Black Tea Extract on Flow Mediated Dilation in Indian Males

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
Study Population
n=30 Participants
All participants who were randomised
Age, Continuous
Age
36 years
n=99 Participants
Sex: Female, Male
Female
0 Participants
n=99 Participants
Sex: Female, Male
Male
30 Participants
n=99 Participants
Region of Enrollment
India
30 participants
n=99 Participants
Body Mass Index
23.2 kg/m^2
n=99 Participants

PRIMARY outcome

Timeframe: Baseline day 1 to 2 hours post consumption on day 8.

Population: Per protocol based on the full data set excluding one subject who did produce relevant data (AE) and three subjects who were removed during blind review (operator comments, hypertension and increase in body weight). Single data points were removed for 6 subjects (all single data points) based on operator comments.

Change in flow mediated dilation (FMD) due to tea consumption when compared to control. FMD measurement included the following steps: * 1 minute base scan to measure the baseline diameter of artery (baseline) * 5 minutes of forearm occlusion at 300±30 mmHg, just below the elbow 2-5 cm from antecubital crease * 4 minutes FMD scan, which started immediately after release of the occlusion (reactive hyperaemia stage) FMD was calculated as maximal percentage change in diameter of the artery above the baseline value after the release of the occlusion

Outcome measures

Outcome measures
Measure
Tea Beverage
n=27 Participants
Participants when they received tea
Control Beverage
n=27 Participants
Participants when they received control
'Acute-upon-chronic Effect' of Tea vs Control
8.03 percentage of change in diameter
Interval 6.95 to 9.12
9.03 percentage of change in diameter
Interval 8.01 to 10.05

SECONDARY outcome

Timeframe: From baseline on day 1 to 2 hours post consumption on day 1

Population: Per protocol based on the full data set excluding one subject who did produce relevant data (AE) and three subjects who were removed during blind review (operator comments, hypertension and increase in body weight). Single data points were removed for 6 subjects (all single data points) based on operator comments.

Change in flow mediated dilation due to tea consumption when compared to control. FMD measurement included the following steps: * 1 minute base scan to measure the baseline diameter of artery (baseline) * 5 minutes of forearm occlusion at 300±30 mmHg, just below the elbow 2-5 cm from antecubital crease * 4 minutes FMD scan, which started immediately after release of the occlusion (reactive hyperaemia stage) FMD was calculated as maximal percentage change in diameter of the artery above the baseline value after the release of the occlusion

Outcome measures

Outcome measures
Measure
Tea Beverage
n=27 Participants
Participants when they received tea
Control Beverage
n=27 Participants
Participants when they received control
Acute Effect of Tea vs Control
8.44 percent change in flow mediated dilation
Interval 7.08 to 9.79
8.82 percent change in flow mediated dilation
Interval 7.52 to 10.09

SECONDARY outcome

Timeframe: From baseline at day 1 to baseline on day 8

Population: Per protocol based on the full data set excluding one subject who did produce relevant data (AE) and three subjects who were removed during blind review (operator comments, hypertension and increase in body weight). Single data points were removed for 6 subjects (all single data points) based on operator comments.

Change in flow mediated dilation (FMD) due to tea consumption when compared to control. FMD measurement included the following steps: * 1 minute base scan to measure the baseline diameter of artery (baseline) * 5 minutes of forearm occlusion at 300±30 mmHg, just below the elbow 2-5 cm from antecubital crease * 4 minutes FMD scan, which started immediately after release of the occlusion (reactive hyperaemia stage) FMD was calculated as maximal percentage change in diameter of the artery above the baseline value after the release of the occlusion

Outcome measures

Outcome measures
Measure
Tea Beverage
n=27 Participants
Participants when they received tea
Control Beverage
n=27 Participants
Participants when they received control
Chronic Effect of Tea vs Control
9.19 Percentage change in flow mediated dilat
Interval 8.28 to 10.09
9.39 Percentage change in flow mediated dilat
Interval 8.51 to 10.27

Adverse Events

Tea Extract

Serious events: 0 serious events
Other events: 1 other events
Deaths: 0 deaths

Control

Serious events: 0 serious events
Other events: 1 other events
Deaths: 0 deaths

Placebo

Serious events: 0 serious events
Other events: 3 other events
Deaths: 0 deaths

Serious adverse events

Adverse event data not reported

Other adverse events

Other adverse events
Measure
Tea Extract
n=29 participants at risk
Subjects when they consumed tea extract for one week
Control
n=29 participants at risk
Subjects when they consumed control for one week
Placebo
n=30 participants at risk
Subjects when they consumed placebo for 6 days during the run in and during 7 days during the washout
Cardiac disorders
Hypertension
0.00%
0/29
0.00%
0/29
3.3%
1/30 • Number of events 1
Infections and infestations
Upper restiratory tract infection
0.00%
0/29
3.4%
1/29 • Number of events 1
3.3%
1/30 • Number of events 1
Gastrointestinal disorders
Increased ferquency of stool
3.4%
1/29 • Number of events 1
0.00%
0/29
0.00%
0/30
Renal and urinary disorders
Blood in urine
0.00%
0/29
0.00%
0/29
3.3%
1/30 • Number of events 1

Additional Information

Dr Theo Mulder

Unilever R&D Vlaardingen

Phone: +31 10 460 8315

Results disclosure agreements

  • Principal investigator is a sponsor employee
  • Publication restrictions are in place