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Tissue Sectioning by Electro-Dissociation

NCT01054612 · Status: WITHDRAWN · Type: OBSERVATIONAL

Last updated 2015-03-04

No results posted yet for this study

Summary

Currently there is no technique to produce thin (0.004-0.01 mm) serial sections of large fresh tissue specimens that are suitable for high-resolution in situ protein/gene expression studies without ice artifact or fixation-induced molecular damage. Traditional frozen sectioning preserves protein and nucleic acid structure, but the inherent ice artifact precludes reconstruction of protein and mRNA expression patterns in 3-dimensions. Since the limitations of the existing sectioning techniques result from the fact that they rely on mechanical cutting which in turn require the tissue to be stiff, we suggest a new approach to cut tissue via an electro erosion process that utilizes focus radio frequency (RF).

Conditions

  • Thin Sections
  • Fresh Unfixed Tissues
  • Fresh Unfrozen Tissues

Interventions

OTHER

Tissue Sectioning via Electro Erosion Process

Focused RF energy can be used to produce consecutive thin sections of fresh tissue for immunohistochemical and nucleic acids analysis by electro-dissociation without thermal damage, ultimately allowing high resolution reconstruction of gene and protein expression patterns of large tissue specimens in 3D.

Sponsors & Collaborators

  • University of Arkansas

    lead OTHER

Principal Investigators

  • Gal Shafirstein, Ph.D. · UAMS, ACH, Central Arkansas Veterans Healthcare System

Eligibility

Sex
ALL
Healthy Volunteers
No

Timeline & Regulatory

Start
2002-03-31
Primary Completion
2010-01-31
Completion
2010-01-31

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Read the full study record

This page highlights key information. For complete eligibility criteria, study locations, investigator contacts, and the full protocol, visit the original record on ClinicalTrials.gov.

View NCT01054612 on ClinicalTrials.gov